Acetoanaerobium sticklandii (strain ATCC 12662 / DSM 519 / JCM 1433 / NCIMB 10654) (Clostridium sticklandii)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Acetoanaerobium; Acetoanaerobium sticklandii

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2572 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3PY22|E3PY22_ACESD Alpha-1 4 glucan phosphorylase OS=Acetoanaerobium sticklandii (strain ATCC 12662 / DSM 519 / JCM 1433 / NCIMB 10654) OX=499177 GN=malP PE=3 SV=1
MM1 pKa = 7.54LVQDD5 pKa = 4.25EE6 pKa = 4.39QQNDD10 pKa = 3.38DD11 pKa = 3.74SLYY14 pKa = 10.91DD15 pKa = 3.4IDD17 pKa = 5.0GLFFIISKK25 pKa = 10.27EE26 pKa = 3.77DD27 pKa = 3.26EE28 pKa = 4.53KK29 pKa = 10.95YY30 pKa = 10.95VKK32 pKa = 10.46YY33 pKa = 10.77IEE35 pKa = 4.73IDD37 pKa = 4.09YY38 pKa = 10.88IDD40 pKa = 4.64DD41 pKa = 3.3WSGADD46 pKa = 3.68FVITAGFF53 pKa = 3.4

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3PWA0|E3PWA0_ACESD Trigger factor OS=Acetoanaerobium sticklandii (strain ATCC 12662 / DSM 519 / JCM 1433 / NCIMB 10654) OX=499177 GN=tig PE=3 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.96RR4 pKa = 11.84TYY6 pKa = 9.62QPKK9 pKa = 9.59KK10 pKa = 7.6RR11 pKa = 11.84QRR13 pKa = 11.84SKK15 pKa = 9.69EE16 pKa = 3.5HH17 pKa = 6.2GFRR20 pKa = 11.84KK21 pKa = 9.76RR22 pKa = 11.84MKK24 pKa = 9.25TSTGRR29 pKa = 11.84NVLRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.72GRR40 pKa = 11.84KK41 pKa = 9.04RR42 pKa = 11.84LTAA45 pKa = 4.18

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2572

0

2572

807997

21

1955

314.2

35.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.362 ± 0.055

0.899 ± 0.017

5.947 ± 0.039

7.549 ± 0.049

4.272 ± 0.033

6.25 ± 0.051

1.528 ± 0.019

9.477 ± 0.047

8.456 ± 0.043

9.223 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.94 ± 0.022

5.449 ± 0.041

3.013 ± 0.029

2.647 ± 0.023

3.384 ± 0.032

6.787 ± 0.041

4.896 ± 0.03

6.383 ± 0.041

0.594 ± 0.013

3.945 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski