Synechococcus phage S-CBP2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Kembevirus; Synechococcus virus SCBP2

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A096VKY0|A0A096VKY0_9CAUD Uncharacterized protein OS=Synechococcus phage S-CBP2 OX=756277 GN=S-CBP2_0005 PE=4 SV=1
MM1 pKa = 7.68TYY3 pKa = 9.14LTQEE7 pKa = 4.38EE8 pKa = 4.74IEE10 pKa = 4.15NLGPEE15 pKa = 4.66EE16 pKa = 4.14YY17 pKa = 10.26CRR19 pKa = 11.84LLAYY23 pKa = 10.45GDD25 pKa = 4.06IEE27 pKa = 6.1LMDD30 pKa = 5.31DD31 pKa = 5.02DD32 pKa = 4.8EE33 pKa = 6.6FSDD36 pKa = 4.05EE37 pKa = 3.8YY38 pKa = 11.36HH39 pKa = 6.78RR40 pKa = 11.84ILRR43 pKa = 11.84HH44 pKa = 5.24FVEE47 pKa = 5.09FDD49 pKa = 2.71II50 pKa = 6.1

Molecular weight: 6.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A096VL47|A0A096VL47_9CAUD Phage_lysozyme2 domain-containing protein OS=Synechococcus phage S-CBP2 OX=756277 GN=S-CBP2_0051 PE=4 SV=1
MM1 pKa = 7.71APQNKK6 pKa = 7.78WFAASPNTDD15 pKa = 3.9YY16 pKa = 10.74IYY18 pKa = 11.08KK19 pKa = 10.35ALTDD23 pKa = 3.56GTVAKK28 pKa = 9.1LTGGRR33 pKa = 11.84IPTMNAAQAAGLVGSWLIEE52 pKa = 4.01TGRR55 pKa = 11.84KK56 pKa = 9.07DD57 pKa = 4.16LRR59 pKa = 11.84NLDD62 pKa = 3.64VVEE65 pKa = 4.72AGTGRR70 pKa = 11.84GRR72 pKa = 11.84GLSQYY77 pKa = 9.33TGVRR81 pKa = 11.84RR82 pKa = 11.84TPYY85 pKa = 10.89DD86 pKa = 3.31KK87 pKa = 10.95AVKK90 pKa = 8.93QARR93 pKa = 11.84AAGQDD98 pKa = 3.6PNSAQWQIKK107 pKa = 9.78YY108 pKa = 9.39FAQEE112 pKa = 4.05YY113 pKa = 8.7MNKK116 pKa = 10.34DD117 pKa = 3.55LIGWTKK123 pKa = 10.41VFEE126 pKa = 4.37KK127 pKa = 10.26MPKK130 pKa = 9.03NLKK133 pKa = 8.9TPGEE137 pKa = 4.04YY138 pKa = 10.39AKK140 pKa = 10.9YY141 pKa = 8.23FTGSAAEE148 pKa = 4.03GKK150 pKa = 10.42GYY152 pKa = 9.98FRR154 pKa = 11.84PGVPHH159 pKa = 6.71TDD161 pKa = 2.6RR162 pKa = 11.84RR163 pKa = 11.84MQAADD168 pKa = 3.31EE169 pKa = 4.44VFRR172 pKa = 11.84HH173 pKa = 5.04YY174 pKa = 10.77AAPNRR179 pKa = 11.84QAPANRR185 pKa = 11.84PAPAGTPAIKK195 pKa = 10.08PGPLKK200 pKa = 10.63QLMNRR205 pKa = 11.84LGIRR209 pKa = 11.84GQDD212 pKa = 2.88QGFAIDD218 pKa = 3.72KK219 pKa = 10.27LSRR222 pKa = 11.84NLGSIAAAPNAGRR235 pKa = 11.84AIFNSIAPGGNSQWKK250 pKa = 9.69SLSKK254 pKa = 10.58ADD256 pKa = 3.51KK257 pKa = 10.29QAWNTAAGQIGAQFGIAINPPTRR280 pKa = 11.84NIGLPTKK287 pKa = 10.22TNVGVNQSFGPKK299 pKa = 9.09PVNLTIRR306 pKa = 11.84GVQPGDD312 pKa = 3.34LGYY315 pKa = 8.92GTNNTRR321 pKa = 11.84VGGLPGLRR329 pKa = 11.84EE330 pKa = 3.71VAQAGIHH337 pKa = 5.73NYY339 pKa = 7.57NWKK342 pKa = 9.36PDD344 pKa = 3.99KK345 pKa = 10.86NWNSYY350 pKa = 7.32TNPHH354 pKa = 5.96YY355 pKa = 10.51NVSRR359 pKa = 11.84SSVHH363 pKa = 5.81NRR365 pKa = 11.84AGSNLRR371 pKa = 11.84GSGGLSTSSGAYY383 pKa = 9.55SGLSVGGYY391 pKa = 10.39GSGNSAYY398 pKa = 10.29RR399 pKa = 11.84GGSVSYY405 pKa = 10.93GSGSVATGTGMGSISRR421 pKa = 11.84GAGWW425 pKa = 3.21

Molecular weight: 45.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

13987

46

1051

263.9

29.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.174 ± 0.396

0.979 ± 0.16

6.056 ± 0.234

6.256 ± 0.452

3.539 ± 0.202

7.636 ± 0.363

1.594 ± 0.189

4.619 ± 0.215

5.105 ± 0.323

8.737 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.227

4.247 ± 0.251

4.333 ± 0.165

5.119 ± 0.416

5.498 ± 0.22

6.327 ± 0.406

6.213 ± 0.42

6.728 ± 0.248

1.416 ± 0.14

3.103 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski