Aliifodinibius roseus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Balneolaeota; Balneolia; Balneolales; Balneolaceae; Aliifodinibius

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4289 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5BL67|A0A1M5BL67_9BACT Starch-binding associating with outer membrane OS=Aliifodinibius roseus OX=1194090 GN=SAMN05443144_108164 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 10.56SIVTKK7 pKa = 10.64LIAILMLPSMLLLASCDD24 pKa = 3.44DD25 pKa = 4.59DD26 pKa = 5.81NPVNPEE32 pKa = 3.99PNPPEE37 pKa = 4.64EE38 pKa = 4.11IPAVYY43 pKa = 10.1EE44 pKa = 4.07DD45 pKa = 5.07LPGEE49 pKa = 5.03LISQDD54 pKa = 3.76EE55 pKa = 4.53TWSNDD60 pKa = 2.94TTLTGPRR67 pKa = 11.84FVLPGVTLTVEE78 pKa = 4.16EE79 pKa = 4.43GVEE82 pKa = 4.5VSFTYY87 pKa = 10.5HH88 pKa = 5.55NQNNDD93 pKa = 3.04EE94 pKa = 4.27VGTIITLPGDD104 pKa = 3.32ATNFDD109 pKa = 4.13EE110 pKa = 4.79PRR112 pKa = 11.84ASGRR116 pKa = 11.84LVAVGTADD124 pKa = 3.31NPIIFTAEE132 pKa = 3.95QKK134 pKa = 10.41EE135 pKa = 4.26VASWGGIILAGEE147 pKa = 4.34ASNNVPGGLGEE158 pKa = 5.28IEE160 pKa = 4.67GLDD163 pKa = 3.36QAVQYY168 pKa = 10.65GADD171 pKa = 3.23IGGGEE176 pKa = 4.49SFNDD180 pKa = 3.28QDD182 pKa = 3.89DD183 pKa = 4.14SGRR186 pKa = 11.84LSYY189 pKa = 11.15VRR191 pKa = 11.84IEE193 pKa = 3.87YY194 pKa = 10.15SGYY197 pKa = 10.4SIADD201 pKa = 3.48GSEE204 pKa = 3.86LQALTLYY211 pKa = 10.73SVGSEE216 pKa = 4.06TQLDD220 pKa = 4.28HH221 pKa = 7.79ISIYY225 pKa = 10.59QSVDD229 pKa = 3.09DD230 pKa = 4.28GVEE233 pKa = 4.09LFGGTVDD240 pKa = 4.35IKK242 pKa = 11.2YY243 pKa = 10.55LVIKK247 pKa = 9.68GAQDD251 pKa = 3.15DD252 pKa = 4.36TFDD255 pKa = 4.56YY256 pKa = 11.09DD257 pKa = 3.75QGWTGRR263 pKa = 11.84GQFWVGVQTEE273 pKa = 4.58GTRR276 pKa = 11.84SNSGFEE282 pKa = 4.58NDD284 pKa = 4.37GCDD287 pKa = 4.77DD288 pKa = 4.57QADD291 pKa = 3.94CDD293 pKa = 4.13GGNGPTAPQIYY304 pKa = 9.75NATIYY309 pKa = 11.17GNDD312 pKa = 3.19VANGEE317 pKa = 4.59DD318 pKa = 3.42IRR320 pKa = 11.84GLRR323 pKa = 11.84LRR325 pKa = 11.84EE326 pKa = 4.51GIEE329 pKa = 3.98GEE331 pKa = 4.2YY332 pKa = 10.88KK333 pKa = 10.78NIIIANFGKK342 pKa = 10.42EE343 pKa = 3.79IIAPIVVDD351 pKa = 4.01EE352 pKa = 5.64DD353 pKa = 3.56GTEE356 pKa = 3.97ANIGSSLTFGGNISYY371 pKa = 11.24NNANDD376 pKa = 4.1GSSLSYY382 pKa = 11.13YY383 pKa = 10.73SDD385 pKa = 4.98LGLQNMDD392 pKa = 3.72PQFVDD397 pKa = 2.97AAGFNFALEE406 pKa = 4.29SDD408 pKa = 4.26SPALTSGVAPPVDD421 pKa = 3.98DD422 pKa = 5.87FFDD425 pKa = 3.5QVEE428 pKa = 4.07YY429 pKa = 11.03SGAFGTEE436 pKa = 3.74DD437 pKa = 2.94WTQEE441 pKa = 4.07GSWVRR446 pKa = 11.84WSDD449 pKa = 3.03

Molecular weight: 48.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5ACZ7|A0A1M5ACZ7_9BACT RNA polymerase sigma subunit ECF family OS=Aliifodinibius roseus OX=1194090 GN=SAMN05443144_10728 PE=4 SV=1
MM1 pKa = 7.71WFFLSGFLTPDD12 pKa = 3.51DD13 pKa = 4.36KK14 pKa = 11.72LSFEE18 pKa = 3.9IKK20 pKa = 9.95LRR22 pKa = 11.84QRR24 pKa = 11.84TALVSLLAPSVARR37 pKa = 11.84VFAVLGRR44 pKa = 11.84TYY46 pKa = 11.13SCLSPLLSASRR57 pKa = 11.84CRR59 pKa = 11.84EE60 pKa = 3.62KK61 pKa = 11.22GKK63 pKa = 10.51GRR65 pKa = 11.84NNIPRR70 pKa = 11.84NRR72 pKa = 11.84ICILLLVRR80 pKa = 11.84DD81 pKa = 4.21DD82 pKa = 3.88TAPP85 pKa = 3.24

Molecular weight: 9.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4289

0

4289

1460705

28

3185

340.6

38.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.108 ± 0.031

0.672 ± 0.011

6.057 ± 0.028

7.6 ± 0.041

4.342 ± 0.024

7.459 ± 0.032

2.133 ± 0.019

6.507 ± 0.034

4.993 ± 0.036

9.268 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.409 ± 0.017

4.467 ± 0.032

4.24 ± 0.022

3.904 ± 0.025

5.497 ± 0.028

6.489 ± 0.031

5.448 ± 0.023

6.311 ± 0.03

1.356 ± 0.016

3.736 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski