Streptomyces phage phiHau3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Arequatrovirus; unclassified Arequatrovirus; Streptomyces virus phiHau3

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4IAX9|K4IAX9_9CAUD Uncharacterized protein OS=Streptomyces phage phiHau3 OX=1204524 GN=phiHau3_3 PE=4 SV=1
MM1 pKa = 6.96YY2 pKa = 10.63QLDD5 pKa = 4.26NEE7 pKa = 4.44TLEE10 pKa = 5.37QIAQQCLAEE19 pKa = 4.28PSNAYY24 pKa = 9.99FRR26 pKa = 11.84DD27 pKa = 3.37DD28 pKa = 3.86RR29 pKa = 11.84LFNTHH34 pKa = 6.22GAVLSWAEE42 pKa = 4.05YY43 pKa = 10.46GDD45 pKa = 4.8SILDD49 pKa = 3.48EE50 pKa = 4.51SNYY53 pKa = 10.31RR54 pKa = 11.84SALDD58 pKa = 4.56LIRR61 pKa = 11.84AAADD65 pKa = 3.47DD66 pKa = 4.36GRR68 pKa = 11.84DD69 pKa = 3.52DD70 pKa = 4.04TEE72 pKa = 4.87ISDD75 pKa = 3.41KK76 pKa = 11.14HH77 pKa = 7.91VIDD80 pKa = 3.67GTSRR84 pKa = 11.84HH85 pKa = 5.05WAVGSLRR92 pKa = 11.84TIYY95 pKa = 10.29VQVRR99 pKa = 11.84EE100 pKa = 4.25DD101 pKa = 3.53YY102 pKa = 10.19EE103 pKa = 4.76RR104 pKa = 11.84CDD106 pKa = 3.6FTGCAGAAMWWRR118 pKa = 11.84EE119 pKa = 3.56GAEE122 pKa = 3.98THH124 pKa = 5.64TQFCEE129 pKa = 4.04DD130 pKa = 3.32HH131 pKa = 6.91RR132 pKa = 11.84EE133 pKa = 3.98EE134 pKa = 4.39YY135 pKa = 10.76DD136 pKa = 3.63ADD138 pKa = 3.8GLSYY142 pKa = 10.92EE143 pKa = 4.2PLIPPFTAAFIEE155 pKa = 4.17AAEE158 pKa = 4.92IITALQDD165 pKa = 3.6YY166 pKa = 8.87PVLDD170 pKa = 4.06EE171 pKa = 4.85SDD173 pKa = 3.86YY174 pKa = 11.61SDD176 pKa = 3.7RR177 pKa = 11.84EE178 pKa = 4.06YY179 pKa = 11.33EE180 pKa = 4.08RR181 pKa = 11.84FQSNLNEE188 pKa = 3.9ALEE191 pKa = 4.15YY192 pKa = 9.51VQRR195 pKa = 11.84DD196 pKa = 3.9FEE198 pKa = 6.93DD199 pKa = 3.68DD200 pKa = 3.62TEE202 pKa = 4.21EE203 pKa = 3.86QSAEE207 pKa = 3.73ISEE210 pKa = 4.28RR211 pKa = 11.84AYY213 pKa = 10.56EE214 pKa = 4.06EE215 pKa = 4.41LGEE218 pKa = 4.66LLGQHH223 pKa = 6.08SDD225 pKa = 2.93GWVDD229 pKa = 3.3SDD231 pKa = 3.76RR232 pKa = 11.84VEE234 pKa = 4.26EE235 pKa = 4.4IYY237 pKa = 10.85RR238 pKa = 11.84EE239 pKa = 3.97ARR241 pKa = 11.84DD242 pKa = 3.47AYY244 pKa = 9.5FTEE247 pKa = 5.17LGNAYY252 pKa = 10.25LNAPIEE258 pKa = 4.46GQLTLVV264 pKa = 3.76

Molecular weight: 30.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4IB61|K4IB61_9CAUD Uncharacterized protein OS=Streptomyces phage phiHau3 OX=1204524 GN=phiHau3_76 PE=4 SV=1
MM1 pKa = 7.48PRR3 pKa = 11.84SGDD6 pKa = 3.33TFTPSASVATPTAPGRR22 pKa = 11.84GGEE25 pKa = 4.36SGTHH29 pKa = 6.08RR30 pKa = 11.84ASPPPPQTGTPSGRR44 pKa = 11.84PACPACPMGCQSRR57 pKa = 11.84PPPVPTFWRR66 pKa = 11.84TPMPVSLSKK75 pKa = 9.73PTDD78 pKa = 3.12RR79 pKa = 11.84VVPDD83 pKa = 3.34VLFLSEE89 pKa = 3.88IARR92 pKa = 11.84EE93 pKa = 4.01IGVTRR98 pKa = 11.84ATVTNWHH105 pKa = 5.73QRR107 pKa = 11.84HH108 pKa = 5.65EE109 pKa = 4.74DD110 pKa = 3.77FPQVHH115 pKa = 7.24AIGGQKK121 pKa = 9.82YY122 pKa = 8.49VSRR125 pKa = 11.84ADD127 pKa = 3.32LFAWLDD133 pKa = 3.54KK134 pKa = 10.74GDD136 pKa = 3.45RR137 pKa = 11.84WEE139 pKa = 4.49KK140 pKa = 10.08IRR142 pKa = 11.84RR143 pKa = 11.84AQTRR147 pKa = 11.84EE148 pKa = 3.55VTEE151 pKa = 3.8RR152 pKa = 11.84KK153 pKa = 9.27PRR155 pKa = 11.84VRR157 pKa = 11.84KK158 pKa = 9.6DD159 pKa = 2.97AAEE162 pKa = 3.88LRR164 pKa = 11.84RR165 pKa = 11.84LIAKK169 pKa = 10.01HH170 pKa = 4.98EE171 pKa = 4.1AALLRR176 pKa = 11.84LNRR179 pKa = 11.84EE180 pKa = 3.97LQRR183 pKa = 11.84ALRR186 pKa = 11.84DD187 pKa = 3.65EE188 pKa = 4.36QVV190 pKa = 2.65

Molecular weight: 21.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

14293

21

1273

198.5

21.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.649 ± 0.418

0.959 ± 0.157

6.402 ± 0.245

7.122 ± 0.436

2.869 ± 0.164

8.263 ± 0.376

1.945 ± 0.213

4.023 ± 0.358

4.03 ± 0.313

8.697 ± 0.469

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.078 ± 0.144

2.519 ± 0.139

5.366 ± 0.375

3.505 ± 0.127

7.087 ± 0.55

5.632 ± 0.282

6.199 ± 0.252

7.227 ± 0.239

1.826 ± 0.109

2.603 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski