Eggerthella sp. CAG:209

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Eggerthella; environmental samples

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1726 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6HII0|R6HII0_9ACTN Probable cell division protein WhiA OS=Eggerthella sp. CAG:209 OX=1262875 GN=whiA PE=3 SV=1
MM1 pKa = 7.39TFDD4 pKa = 5.17EE5 pKa = 5.67LDD7 pKa = 3.63EE8 pKa = 4.32QQTKK12 pKa = 9.72AALYY16 pKa = 9.76VLEE19 pKa = 4.97LADD22 pKa = 4.7IEE24 pKa = 4.36PTRR27 pKa = 11.84EE28 pKa = 3.55NARR31 pKa = 11.84LYY33 pKa = 10.14LAWDD37 pKa = 3.52VLSFTEE43 pKa = 4.45DD44 pKa = 3.09AQGRR48 pKa = 11.84YY49 pKa = 7.54CWYY52 pKa = 9.65MDD54 pKa = 3.95DD55 pKa = 4.89EE56 pKa = 4.89GNEE59 pKa = 3.79ACIKK63 pKa = 10.24VDD65 pKa = 3.28SLEE68 pKa = 4.33IIEE71 pKa = 4.25TSEE74 pKa = 4.1YY75 pKa = 10.4EE76 pKa = 3.91

Molecular weight: 8.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6I826|R6I826_9ACTN FHA domain-containing protein OS=Eggerthella sp. CAG:209 OX=1262875 GN=BN534_00314 PE=4 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84IIAGFRR8 pKa = 11.84QVFEE12 pKa = 4.4RR13 pKa = 11.84EE14 pKa = 4.11IIGTTTSVEE23 pKa = 4.27VTCAQIHH30 pKa = 6.44CIGASFYY37 pKa = 11.33GCVQTFHH44 pKa = 6.14VASRR48 pKa = 11.84GQKK51 pKa = 9.63LHH53 pKa = 5.28VLRR56 pKa = 11.84ARR58 pKa = 11.84SSHH61 pKa = 5.89HH62 pKa = 6.46ASFVLLIGFLVHH74 pKa = 6.6GFVSHH79 pKa = 6.21TFVLKK84 pKa = 10.54PLDD87 pKa = 3.71KK88 pKa = 10.73ARR90 pKa = 11.84KK91 pKa = 8.06RR92 pKa = 11.84PSSSMMAVSLHH103 pKa = 6.76CYY105 pKa = 9.43AALRR109 pKa = 4.07

Molecular weight: 12.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1726

0

1726

519372

30

1543

300.9

32.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.366 ± 0.08

1.73 ± 0.031

5.627 ± 0.054

6.855 ± 0.066

4.022 ± 0.047

7.824 ± 0.059

2.006 ± 0.031

5.717 ± 0.051

4.689 ± 0.046

9.109 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.031

3.478 ± 0.033

4.033 ± 0.034

3.175 ± 0.031

5.367 ± 0.057

6.467 ± 0.063

5.21 ± 0.046

7.796 ± 0.059

0.954 ± 0.024

2.878 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski