Helicosporidium sp. ATCC 50920

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae; Chlorellales; Chlorellaceae; Helicosporidium; unclassified Helicosporidium

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6029 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059LJF9|A0A059LJF9_9CHLO Uncharacterized protein OS=Helicosporidium sp. ATCC 50920 OX=1291522 GN=H632_c1402p0 PE=4 SV=1
MM1 pKa = 7.87DD2 pKa = 5.16PAAEE6 pKa = 4.05HH7 pKa = 6.02FKK9 pKa = 11.08YY10 pKa = 10.04PVSIDD15 pKa = 2.88IRR17 pKa = 11.84EE18 pKa = 4.59LITLEE23 pKa = 5.53DD24 pKa = 3.63VMQEE28 pKa = 4.17LDD30 pKa = 3.57LGPNGGLLYY39 pKa = 10.74CMEE42 pKa = 4.23YY43 pKa = 9.78MEE45 pKa = 4.55EE46 pKa = 5.84HH47 pKa = 6.48LDD49 pKa = 3.31EE50 pKa = 4.48WLGQEE55 pKa = 4.4LEE57 pKa = 4.54SFGEE61 pKa = 3.95DD62 pKa = 3.46DD63 pKa = 5.49YY64 pKa = 12.21LLFDD68 pKa = 4.49CPGQIEE74 pKa = 5.2LYY76 pKa = 9.47SHH78 pKa = 6.64VNVFRR83 pKa = 11.84SFVDD87 pKa = 4.11FLRR90 pKa = 11.84KK91 pKa = 10.19DD92 pKa = 3.18GWQVCAVYY100 pKa = 10.83CMDD103 pKa = 3.58AQFVAEE109 pKa = 4.26APKK112 pKa = 10.65FIGGCLSALSAMVHH126 pKa = 6.5LEE128 pKa = 3.98LPHH131 pKa = 8.34INVLTKK137 pKa = 10.63ADD139 pKa = 5.04LIQNKK144 pKa = 8.85EE145 pKa = 3.95VLDD148 pKa = 4.87DD149 pKa = 3.75YY150 pKa = 11.22LFPSVEE156 pKa = 4.17DD157 pKa = 3.5MQHH160 pKa = 6.98RR161 pKa = 11.84LDD163 pKa = 3.88TTTAPHH169 pKa = 6.76LRR171 pKa = 11.84PLNAAMAQLVDD182 pKa = 3.55QFGLVSFVSLDD193 pKa = 3.28ITDD196 pKa = 4.01EE197 pKa = 4.06EE198 pKa = 5.07SIEE201 pKa = 4.01QLLLQVDD208 pKa = 3.72STIQYY213 pKa = 11.04GEE215 pKa = 4.08DD216 pKa = 3.09QEE218 pKa = 5.13VRR220 pKa = 11.84TKK222 pKa = 10.78EE223 pKa = 3.94YY224 pKa = 11.04GDD226 pKa = 4.08FPDD229 pKa = 4.49EE230 pKa = 4.06EE231 pKa = 4.44SAAAAGEE238 pKa = 4.11QEE240 pKa = 4.7LDD242 pKa = 3.63FF243 pKa = 6.37

Molecular weight: 27.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059LAK7|A0A059LAK7_9CHLO Glucose inhibited division protein A (Fragment) OS=Helicosporidium sp. ATCC 50920 OX=1291522 GN=H632_c5143p0 PE=3 SV=1
RR1 pKa = 7.05QALPRR6 pKa = 11.84ARR8 pKa = 11.84VGPLGRR14 pKa = 11.84LLGKK18 pKa = 10.05KK19 pKa = 8.89RR20 pKa = 11.84ASGRR24 pKa = 11.84GVDD27 pKa = 4.05GGAGGAARR35 pKa = 11.84PAPGRR40 pKa = 11.84GGGPGGARR48 pKa = 11.84AGVVLRR54 pKa = 11.84ARR56 pKa = 11.84LRR58 pKa = 11.84VEE60 pKa = 3.9RR61 pKa = 11.84RR62 pKa = 11.84AAQAGGRR69 pKa = 11.84GQLRR73 pKa = 11.84GGSRR77 pKa = 11.84FANGLGAAPRR87 pKa = 11.84APALLGSPSSRR98 pKa = 11.84ARR100 pKa = 11.84VAGGEE105 pKa = 3.95PGRR108 pKa = 11.84PGALRR113 pKa = 11.84RR114 pKa = 11.84ALWPGRR120 pKa = 11.84PGRR123 pKa = 11.84GRR125 pKa = 11.84LSQPLRR131 pKa = 11.84PLGRR135 pKa = 11.84RR136 pKa = 11.84WQGAARR142 pKa = 11.84PARR145 pKa = 11.84RR146 pKa = 11.84ARR148 pKa = 11.84RR149 pKa = 11.84ARR151 pKa = 11.84GRR153 pKa = 11.84PRR155 pKa = 11.84PAARR159 pKa = 11.84ARR161 pKa = 11.84GRR163 pKa = 11.84LASLL167 pKa = 3.83

Molecular weight: 17.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6029

0

6029

1647305

8

2258

273.2

29.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.754 ± 0.05

1.548 ± 0.016

5.203 ± 0.023

6.626 ± 0.042

3.157 ± 0.018

8.295 ± 0.039

2.23 ± 0.016

2.802 ± 0.021

3.395 ± 0.03

10.198 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.992 ± 0.015

2.151 ± 0.017

5.997 ± 0.027

3.598 ± 0.019

7.749 ± 0.045

7.149 ± 0.034

4.316 ± 0.022

7.202 ± 0.029

1.455 ± 0.013

2.183 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski