Lake Sarah-associated circular virus-42

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A140AQQ8|A0A140AQQ8_9VIRU Coat protein OS=Lake Sarah-associated circular virus-42 OX=1685771 PE=4 SV=1
MM1 pKa = 7.42SRR3 pKa = 11.84GKK5 pKa = 9.38SWCFTIHH12 pKa = 6.6SRR14 pKa = 11.84GEE16 pKa = 3.95GDD18 pKa = 3.41DD19 pKa = 4.79CDD21 pKa = 4.08WLLHH25 pKa = 6.3PIGTAEE31 pKa = 4.35PPSAFWDD38 pKa = 3.81DD39 pKa = 5.84DD40 pKa = 3.96PFEE43 pKa = 4.51WGCQFMIFQMEE54 pKa = 4.53RR55 pKa = 11.84CPEE58 pKa = 3.95TGKK61 pKa = 10.25LHH63 pKa = 6.41LQGALQLDD71 pKa = 3.82KK72 pKa = 10.83QQRR75 pKa = 11.84LSFMKK80 pKa = 10.32KK81 pKa = 7.85LHH83 pKa = 6.07KK84 pKa = 8.13TAHH87 pKa = 5.58WEE89 pKa = 4.07VMKK92 pKa = 10.98GNWTQSIEE100 pKa = 4.17YY101 pKa = 9.49CSKK104 pKa = 11.16SEE106 pKa = 4.15TKK108 pKa = 10.89VNGPWEE114 pKa = 4.34AGDD117 pKa = 3.8RR118 pKa = 11.84PKK120 pKa = 10.32MGQRR124 pKa = 11.84TDD126 pKa = 3.38LEE128 pKa = 4.9TIGKK132 pKa = 7.56MVKK135 pKa = 9.52EE136 pKa = 3.98NKK138 pKa = 9.25TNLEE142 pKa = 4.08LVDD145 pKa = 3.65TLGAGVSKK153 pKa = 9.79FQKK156 pKa = 9.99HH157 pKa = 4.44ISFLRR162 pKa = 11.84FTYY165 pKa = 9.9SEE167 pKa = 4.3KK168 pKa = 11.05DD169 pKa = 3.03SDD171 pKa = 3.85RR172 pKa = 11.84QATGVKK178 pKa = 10.15VIVLYY183 pKa = 11.07GPTGTGKK190 pKa = 8.99TYY192 pKa = 10.95AAVNSLTGGRR202 pKa = 11.84AYY204 pKa = 10.49HH205 pKa = 6.72ILNAPSQKK213 pKa = 9.11ATKK216 pKa = 9.64LWFDD220 pKa = 3.52GYY222 pKa = 10.92EE223 pKa = 3.98NQKK226 pKa = 10.26VLVIDD231 pKa = 4.82DD232 pKa = 4.01FDD234 pKa = 6.0GSVEE238 pKa = 3.85FRR240 pKa = 11.84YY241 pKa = 9.93LLRR244 pKa = 11.84MIDD247 pKa = 3.69VYY249 pKa = 11.21KK250 pKa = 10.75FPAEE254 pKa = 4.31VKK256 pKa = 10.45GGMVWGVWDD265 pKa = 3.82TVIITSNVHH274 pKa = 5.02PASWYY279 pKa = 8.69TGVDD283 pKa = 3.22TSPLKK288 pKa = 10.61RR289 pKa = 11.84RR290 pKa = 11.84IAEE293 pKa = 3.62IRR295 pKa = 11.84LCEE298 pKa = 3.93NQGTYY303 pKa = 11.17KK304 pKa = 9.94MISWEE309 pKa = 4.14EE310 pKa = 4.01TVLSNDD316 pKa = 3.57FEE318 pKa = 4.66NFF320 pKa = 3.09

Molecular weight:
36.57 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A140AQQ8|A0A140AQQ8_9VIRU Coat protein OS=Lake Sarah-associated circular virus-42 OX=1685771 PE=4 SV=1
MM1 pKa = 7.5MYY3 pKa = 10.22SQGAMSQRR11 pKa = 11.84RR12 pKa = 11.84TRR14 pKa = 11.84TYY16 pKa = 9.09GARR19 pKa = 11.84RR20 pKa = 11.84YY21 pKa = 8.8PYY23 pKa = 10.03RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84TTSLYY31 pKa = 9.54RR32 pKa = 11.84SPYY35 pKa = 8.0TRR37 pKa = 11.84RR38 pKa = 11.84QDD40 pKa = 3.41MVPTTQGWSFNRR52 pKa = 11.84LNRR55 pKa = 11.84SSGGAQRR62 pKa = 11.84VEE64 pKa = 4.02WKK66 pKa = 10.55FSDD69 pKa = 3.57RR70 pKa = 11.84YY71 pKa = 10.44EE72 pKa = 4.83IGLTTMPLIGQFYY85 pKa = 9.85LLNGLVPGSGASQRR99 pKa = 11.84IGTMITMKK107 pKa = 10.03TVEE110 pKa = 4.6TRR112 pKa = 11.84AIWIPFSSATIGLFRR127 pKa = 11.84FFIVLDD133 pKa = 3.67HH134 pKa = 6.17QANGVTPLLSDD145 pKa = 3.33IMAATGIQFASGLRR159 pKa = 11.84NLNTRR164 pKa = 11.84QRR166 pKa = 11.84YY167 pKa = 9.02RR168 pKa = 11.84ILMDD172 pKa = 2.96KK173 pKa = 10.46TYY175 pKa = 11.07GFNNPNFTTSQPYY188 pKa = 9.94YY189 pKa = 7.4MHH191 pKa = 7.03HH192 pKa = 6.03YY193 pKa = 9.08MRR195 pKa = 11.84FRR197 pKa = 11.84LPVTTQYY204 pKa = 11.69NSGSAGSVGDD214 pKa = 3.51IVTNALYY221 pKa = 10.26FIATTTAAASATPSFQMLCRR241 pKa = 11.84LRR243 pKa = 11.84YY244 pKa = 8.18TDD246 pKa = 3.33AA247 pKa = 5.99

Molecular weight:
28.15 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

567

247

320

283.5

32.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.82 ± 0.908

1.235 ± 0.514

4.762 ± 1.193

4.233 ± 1.868

4.938 ± 0.201

8.113 ± 0.26

1.94 ± 0.449

4.938 ± 0.201

4.938 ± 2.305

7.231 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.88 ± 0.606

3.88 ± 0.355

4.056 ± 0.246

4.586 ± 0.419

7.055 ± 2.149

7.055 ± 0.645

8.995 ± 1.449

5.115 ± 0.91

2.646 ± 0.886

4.586 ± 1.171

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski