Agrobacterium phage Atu_ph02

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Atuphduovirus; Agrobacterium virus Atuph02

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L0UYY1|A0A2L0UYY1_9CAUD Uncharacterized protein OS=Agrobacterium phage Atu_ph02 OX=2024261 PE=4 SV=1
MM1 pKa = 8.43DD2 pKa = 3.88YY3 pKa = 11.01TDD5 pKa = 5.58LSDD8 pKa = 3.42EE9 pKa = 4.59SKK11 pKa = 10.65ISLAIEE17 pKa = 4.29FVAIGQPLPDD27 pKa = 4.85VLASWLKK34 pKa = 11.31DD35 pKa = 3.05NGLYY39 pKa = 10.69DD40 pKa = 6.28LITKK44 pKa = 9.96PGFNAGLPDD53 pKa = 3.83GNAA56 pKa = 3.36

Molecular weight: 6.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L0UYY2|A0A2L0UYY2_9CAUD Uncharacterized protein OS=Agrobacterium phage Atu_ph02 OX=2024261 PE=4 SV=1
MM1 pKa = 7.59EE2 pKa = 5.46LLIFILIALMTVIGLLIMMARR23 pKa = 11.84LHH25 pKa = 6.61LKK27 pKa = 10.43RR28 pKa = 11.84FLGYY32 pKa = 9.85PNAVDD37 pKa = 3.35IGATIVFTMLFHH49 pKa = 6.13GTFAGMVVVGFASLMLSLSLWALRR73 pKa = 11.84SSIGCEE79 pKa = 3.45RR80 pKa = 11.84LAVRR84 pKa = 11.84YY85 pKa = 10.02GRR87 pKa = 11.84MGRR90 pKa = 11.84PIVYY94 pKa = 8.98WKK96 pKa = 8.85TIPASEE102 pKa = 4.75CAPHH106 pKa = 5.76WLARR110 pKa = 11.84IFSINNPLKK119 pKa = 10.46GAHH122 pKa = 5.99AA123 pKa = 4.65

Molecular weight: 13.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

14004

37

1254

254.6

28.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.454 ± 0.644

0.621 ± 0.129

6.605 ± 0.234

5.827 ± 0.282

3.37 ± 0.21

7.705 ± 0.295

1.949 ± 0.193

5.063 ± 0.19

5.62 ± 0.375

7.741 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.771 ± 0.183

4.156 ± 0.23

4.427 ± 0.187

4.484 ± 0.252

6.07 ± 0.218

5.298 ± 0.259

5.905 ± 0.243

6.941 ± 0.291

1.75 ± 0.127

3.242 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski