Roseobacter phage DSS3P8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0UXN0|A0A1B0UXN0_9CAUD Uncharacterized protein OS=Roseobacter phage DSS3P8 OX=1735562 GN=DSS3P8_124 PE=4 SV=1
MM1 pKa = 7.55FFPNNVSDD9 pKa = 3.66QSISVFFAGGMRR21 pKa = 11.84SIPASDD27 pKa = 3.58PGFAEE32 pKa = 4.9LSAHH36 pKa = 6.62LALGEE41 pKa = 4.01HH42 pKa = 7.4DD43 pKa = 4.25YY44 pKa = 10.46EE45 pKa = 4.52TVEE48 pKa = 4.7RR49 pKa = 11.84LVDD52 pKa = 3.77KK53 pKa = 10.68PKK55 pKa = 10.27MIARR59 pKa = 11.84LSEE62 pKa = 3.79GLVQVIGSTVYY73 pKa = 10.71YY74 pKa = 10.01KK75 pKa = 9.68GTPAHH80 pKa = 5.58STIAYY85 pKa = 9.04RR86 pKa = 11.84LLDD89 pKa = 3.8ILNAGQNAQVWARR102 pKa = 11.84FLEE105 pKa = 4.12RR106 pKa = 11.84VMANPSARR114 pKa = 11.84SRR116 pKa = 11.84QCLYY120 pKa = 10.95EE121 pKa = 5.68FLDD124 pKa = 3.39AWKK127 pKa = 10.72APLTEE132 pKa = 5.12DD133 pKa = 3.67GCFITFKK140 pKa = 10.43RR141 pKa = 11.84VRR143 pKa = 11.84ADD145 pKa = 3.05YY146 pKa = 11.07RR147 pKa = 11.84DD148 pKa = 3.29IHH150 pKa = 7.15SGKK153 pKa = 9.9FDD155 pKa = 3.69NSPGQTVEE163 pKa = 3.84VSRR166 pKa = 11.84DD167 pKa = 3.63EE168 pKa = 5.07VDD170 pKa = 4.36DD171 pKa = 6.17DD172 pKa = 4.3PDD174 pKa = 3.71EE175 pKa = 4.51TCSYY179 pKa = 9.18GLHH182 pKa = 5.65VAATSYY188 pKa = 10.71LGHH191 pKa = 7.05HH192 pKa = 6.02YY193 pKa = 10.6VSSPSYY199 pKa = 9.52NTIACKK205 pKa = 10.15VDD207 pKa = 3.37PADD210 pKa = 3.62VVAVPRR216 pKa = 11.84DD217 pKa = 3.51YY218 pKa = 11.51GSAKK222 pKa = 9.47MRR224 pKa = 11.84VCKK227 pKa = 10.59YY228 pKa = 10.36VVLGDD233 pKa = 4.06AEE235 pKa = 4.06EE236 pKa = 4.33SFYY239 pKa = 11.77NNAEE243 pKa = 3.97SSPIVPLSMTTSDD256 pKa = 4.19DD257 pKa = 3.67MAPEE261 pKa = 4.49VYY263 pKa = 10.62VDD265 pKa = 5.89DD266 pKa = 6.0DD267 pKa = 6.07FEE269 pKa = 7.2DD270 pKa = 3.78EE271 pKa = 4.52DD272 pKa = 4.68NEE274 pKa = 5.22DD275 pKa = 5.2RR276 pKa = 11.84ICPDD280 pKa = 3.35CGDD283 pKa = 4.62YY284 pKa = 10.88KK285 pKa = 11.28ADD287 pKa = 3.27WQAICEE293 pKa = 4.22DD294 pKa = 4.36CEE296 pKa = 3.96DD297 pKa = 3.87ARR299 pKa = 11.84NEE301 pKa = 4.11EE302 pKa = 4.14EE303 pKa = 5.44DD304 pKa = 3.7IPYY307 pKa = 10.44CGNCGNVEE315 pKa = 3.87VDD317 pKa = 5.39EE318 pKa = 5.33EE319 pKa = 5.35DD320 pKa = 4.4DD321 pKa = 3.87LCDD324 pKa = 3.18RR325 pKa = 11.84CEE327 pKa = 4.15QEE329 pKa = 5.48EE330 pKa = 4.04HH331 pKa = 7.37AEE333 pKa = 4.0WQEE336 pKa = 3.7QWDD339 pKa = 3.59ADD341 pKa = 3.48ARR343 pKa = 11.84AMEE346 pKa = 4.6EE347 pKa = 4.01EE348 pKa = 4.35DD349 pKa = 4.85CIAEE353 pKa = 4.32TGCTLNGVPVSDD365 pKa = 5.51DD366 pKa = 3.43PSDD369 pKa = 3.97YY370 pKa = 11.53DD371 pKa = 5.77DD372 pKa = 5.4IPDD375 pKa = 3.89TEE377 pKa = 4.13ASEE380 pKa = 4.22MAFMRR385 pKa = 11.84GGVTYY390 pKa = 9.87QASEE394 pKa = 4.01IMVGIANHH402 pKa = 5.15GQRR405 pKa = 11.84GYY407 pKa = 11.49SRR409 pKa = 11.84MTGIPRR415 pKa = 11.84TTLQDD420 pKa = 2.84WTTKK424 pKa = 10.23IEE426 pKa = 3.99ACEE429 pKa = 3.77

Molecular weight: 47.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0V1V5|A0A1B0V1V5_9CAUD Uncharacterized protein OS=Roseobacter phage DSS3P8 OX=1735562 GN=DSS3P8_003 PE=4 SV=1
MM1 pKa = 7.69GSPVHH6 pKa = 6.92ACQCKK11 pKa = 9.2DD12 pKa = 3.28CKK14 pKa = 9.97KK15 pKa = 10.46ARR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 10.49GLVLGSRR26 pKa = 11.84RR27 pKa = 11.84MGFWCARR34 pKa = 11.84IIGYY38 pKa = 8.95GVEE41 pKa = 4.32YY42 pKa = 10.91YY43 pKa = 10.48LGSVSAYY50 pKa = 9.88VPHH53 pKa = 7.19PRR55 pKa = 11.84LAKK58 pKa = 9.7GKK60 pKa = 9.71RR61 pKa = 11.84LRR63 pKa = 11.84RR64 pKa = 11.84EE65 pKa = 3.68QNKK68 pKa = 9.01LQRR71 pKa = 11.84EE72 pKa = 4.02IRR74 pKa = 11.84TNMEE78 pKa = 3.89DD79 pKa = 3.06

Molecular weight: 9.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

229

0

229

40708

28

1825

177.8

19.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.634 ± 0.417

1.098 ± 0.088

6.755 ± 0.156

6.846 ± 0.197

3.913 ± 0.109

7.753 ± 0.218

2.076 ± 0.116

5.431 ± 0.139

4.532 ± 0.199

7.335 ± 0.147

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.225 ± 0.096

3.562 ± 0.113

4.267 ± 0.181

2.867 ± 0.16

5.974 ± 0.138

5.64 ± 0.243

6.281 ± 0.167

6.994 ± 0.13

1.619 ± 0.078

3.196 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski