bacterium D16-59

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5054 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A8WJ90|A0A3A8WJ90_9BACT XRE family transcriptional regulator OS=bacterium D16-59 OX=2320098 GN=D7V96_17805 PE=4 SV=1
MM1 pKa = 7.79ANVTLDD7 pKa = 3.81AEE9 pKa = 4.42TLYY12 pKa = 9.13YY13 pKa = 10.75QYY15 pKa = 10.31MINHH19 pKa = 6.29NPTSTMLNAISGNSSDD35 pKa = 4.89DD36 pKa = 3.5SSLGLMGSLGALNSLGSLDD55 pKa = 3.89ALGMSGLSGISGALGSLDD73 pKa = 3.2SSGSVLSFANVLEE86 pKa = 4.23QCLSSAKK93 pKa = 8.93ATAYY97 pKa = 9.62TEE99 pKa = 4.34SIEE102 pKa = 4.08AAEE105 pKa = 4.32MADD108 pKa = 3.44KK109 pKa = 10.85LSSVLEE115 pKa = 4.19EE116 pKa = 4.04AAGTEE121 pKa = 4.43DD122 pKa = 4.42TSSLTYY128 pKa = 9.29KK129 pKa = 9.79TVQEE133 pKa = 4.03LYY135 pKa = 9.89EE136 pKa = 4.14YY137 pKa = 10.47FSEE140 pKa = 4.47KK141 pKa = 10.37VSSKK145 pKa = 10.54AASLLGDD152 pKa = 3.39AAGTGKK158 pKa = 10.61AGTTSAASSAGGVASSVEE176 pKa = 4.11QMNQAAMKK184 pKa = 9.73GQEE187 pKa = 3.96FDD189 pKa = 3.78FSEE192 pKa = 4.12MDD194 pKa = 3.4AMVDD198 pKa = 3.44AAFEE202 pKa = 4.19EE203 pKa = 4.3QMPLSS208 pKa = 3.96

Molecular weight: 21.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A8W219|A0A3A8W219_9BACT Uncharacterized protein OS=bacterium D16-59 OX=2320098 GN=D7V96_23845 PE=4 SV=1
MM1 pKa = 7.32PRR3 pKa = 11.84WHH5 pKa = 6.84KK6 pKa = 7.49MTWKK10 pKa = 10.08GFVPRR15 pKa = 11.84WHH17 pKa = 6.31KK18 pKa = 8.08VTWRR22 pKa = 11.84EE23 pKa = 4.6FVPRR27 pKa = 11.84WHH29 pKa = 6.47KK30 pKa = 7.97VTWRR34 pKa = 11.84GFVPRR39 pKa = 11.84WHH41 pKa = 6.36KK42 pKa = 8.49VTWKK46 pKa = 10.2GFVPRR51 pKa = 11.84WHH53 pKa = 6.37KK54 pKa = 8.49VTWKK58 pKa = 10.2GFVPRR63 pKa = 11.84WHH65 pKa = 6.58KK66 pKa = 11.02VII68 pKa = 4.51

Molecular weight: 8.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5054

0

5054

1488312

19

2992

294.5

33.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.133 ± 0.037

1.581 ± 0.016

5.446 ± 0.027

7.922 ± 0.048

4.258 ± 0.025

6.847 ± 0.032

1.657 ± 0.017

7.268 ± 0.037

7.737 ± 0.036

8.788 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.99 ± 0.018

4.513 ± 0.027

2.978 ± 0.024

3.469 ± 0.023

4.433 ± 0.028

6.048 ± 0.032

5.017 ± 0.028

6.529 ± 0.031

0.96 ± 0.012

4.426 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski