Synechococcus phage S-CBP4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Poseidonvirus; Synechococcus virus SCBP4

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A096VKQ9|A0A096VKQ9_9CAUD Uncharacterized protein OS=Synechococcus phage S-CBP4 OX=754059 GN=S-CBP4_0044 PE=4 SV=1
MM1 pKa = 7.7HH2 pKa = 7.95DD3 pKa = 3.51ALTARR8 pKa = 11.84FTDD11 pKa = 3.23ADD13 pKa = 4.34EE14 pKa = 4.83IKK16 pKa = 10.84DD17 pKa = 3.66VANYY21 pKa = 9.05GCSGGVSGFIYY32 pKa = 10.52YY33 pKa = 10.03SEE35 pKa = 4.06NEE37 pKa = 4.14KK38 pKa = 10.92FFDD41 pKa = 3.81EE42 pKa = 4.89YY43 pKa = 11.05EE44 pKa = 4.24DD45 pKa = 4.74EE46 pKa = 5.36IYY48 pKa = 10.52DD49 pKa = 3.92YY50 pKa = 11.81LNDD53 pKa = 4.48CEE55 pKa = 5.47LSMVDD60 pKa = 5.52FVDD63 pKa = 3.21TGSTIRR69 pKa = 11.84SLKK72 pKa = 10.18NDD74 pKa = 3.73LVWAVVEE81 pKa = 4.29AWCYY85 pKa = 9.39AQHH88 pKa = 6.18TVNEE92 pKa = 4.36MEE94 pKa = 4.79AEE96 pKa = 3.91ALAAA100 pKa = 4.68

Molecular weight: 11.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1NXU0|M1NXU0_9CAUD Uncharacterized protein OS=Synechococcus phage S-CBP4 OX=754059 GN=S-CBP4_0022 PE=4 SV=1
MM1 pKa = 7.84AKK3 pKa = 10.0PGLYY7 pKa = 10.66ANIHH11 pKa = 6.06AKK13 pKa = 9.75RR14 pKa = 11.84KK15 pKa = 9.73RR16 pKa = 11.84IAAGSGEE23 pKa = 4.29KK24 pKa = 9.08MRR26 pKa = 11.84KK27 pKa = 8.92PGAKK31 pKa = 9.54GAPTAAQFKK40 pKa = 9.9KK41 pKa = 10.38AAKK44 pKa = 7.6TAKK47 pKa = 9.66KK48 pKa = 10.3NSIGKK53 pKa = 9.06SVNTARR59 pKa = 11.84VLADD63 pKa = 3.19

Molecular weight: 6.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

11686

38

1314

254.0

28.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.413 ± 0.455

0.779 ± 0.182

6.007 ± 0.324

6.11 ± 0.414

3.697 ± 0.233

7.222 ± 0.533

1.275 ± 0.187

5.519 ± 0.18

5.126 ± 0.338

8.429 ± 0.336

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.49 ± 0.22

5.057 ± 0.28

4.33 ± 0.207

4.955 ± 0.518

5.057 ± 0.314

6.948 ± 0.432

6.435 ± 0.472

6.221 ± 0.243

1.266 ± 0.16

3.663 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski