Lactococcus phage TP712

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059NT69|A0A059NT69_9CAUD TerL OS=Lactococcus phage TP712 OX=314021 PE=4 SV=1
MM1 pKa = 7.08SVKK4 pKa = 9.84VHH6 pKa = 6.15FSNGEE11 pKa = 4.04SIVISEE17 pKa = 4.5EE18 pKa = 3.94TRR20 pKa = 11.84ISAWNSLDD28 pKa = 3.64KK29 pKa = 11.49DD30 pKa = 3.32PDD32 pKa = 3.46GYY34 pKa = 10.6YY35 pKa = 10.77AEE37 pKa = 5.18GVFSGSNIDD46 pKa = 4.29SPDD49 pKa = 3.38LGTSYY54 pKa = 10.29QHH56 pKa = 6.52IGLMGLFGSTDD67 pKa = 2.74WFAIGLDD74 pKa = 3.72FKK76 pKa = 9.31TTYY79 pKa = 8.25KK80 pKa = 10.22TSAIVSLEE88 pKa = 3.88EE89 pKa = 4.31TPWW92 pKa = 4.61

Molecular weight: 10.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059NTA2|A0A059NTA2_9CAUD Uncharacterized protein OS=Lactococcus phage TP712 OX=314021 PE=4 SV=1
MM1 pKa = 7.67GEE3 pKa = 4.16IKK5 pKa = 10.4RR6 pKa = 11.84SFPKK10 pKa = 10.14RR11 pKa = 11.84GRR13 pKa = 11.84FFLDD17 pKa = 2.87NGNGKK22 pKa = 10.23LYY24 pKa = 10.97NFYY27 pKa = 10.45IPKK30 pKa = 8.38NTFLINSFLSVPLLTGALFLCYY52 pKa = 10.07NIVGMFVRR60 pKa = 11.84FHH62 pKa = 7.12PIPRR66 pKa = 11.84VKK68 pKa = 10.41PFFGVAFFIYY78 pKa = 10.39

Molecular weight: 9.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12571

52

1566

216.7

24.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.69 ± 0.553

0.485 ± 0.099

6.173 ± 0.213

7.191 ± 0.531

4.272 ± 0.23

6.427 ± 0.616

1.376 ± 0.134

7.438 ± 0.234

8.981 ± 0.366

8.186 ± 0.358

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.299 ± 0.173

6.133 ± 0.331

2.705 ± 0.129

4.105 ± 0.204

3.564 ± 0.308

7.072 ± 0.348

5.982 ± 0.36

5.759 ± 0.3

1.169 ± 0.135

3.993 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski