Bacillus mesophilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4152 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M0QBE8|A0A6M0QBE8_9BACI Pyridoxamine 5'-phosphate oxidase OS=Bacillus mesophilus OX=1808955 GN=G4D63_18175 PE=4 SV=1
MM1 pKa = 7.44SLEE4 pKa = 3.87WFHH7 pKa = 8.81RR8 pKa = 11.84MEE10 pKa = 4.11NAVRR14 pKa = 11.84ISLPDD19 pKa = 3.25LCEE22 pKa = 4.38SFDD25 pKa = 3.57EE26 pKa = 5.12FEE28 pKa = 5.19ILFDD32 pKa = 4.48TNTTTYY38 pKa = 9.32NPEE41 pKa = 4.27YY42 pKa = 9.64IFSIQTVDD50 pKa = 4.05DD51 pKa = 4.31VEE53 pKa = 4.5DD54 pKa = 3.78FCIISFDD61 pKa = 3.61PTNQEE66 pKa = 3.72FFSFYY71 pKa = 10.39YY72 pKa = 10.5HH73 pKa = 7.25EE74 pKa = 4.72EE75 pKa = 3.74LDD77 pKa = 3.71IQSKK81 pKa = 9.95VLFRR85 pKa = 11.84DD86 pKa = 2.99ADD88 pKa = 4.06EE89 pKa = 4.2ILGYY93 pKa = 9.97VHH95 pKa = 7.0ATFHH99 pKa = 6.73EE100 pKa = 4.7YY101 pKa = 10.11IGDD104 pKa = 3.56IDD106 pKa = 4.07EE107 pKa = 5.86DD108 pKa = 3.94EE109 pKa = 4.86LLEE112 pKa = 4.57EE113 pKa = 5.61DD114 pKa = 4.97LDD116 pKa = 3.97EE117 pKa = 5.52LGNHH121 pKa = 6.74LYY123 pKa = 10.91EE124 pKa = 5.26EE125 pKa = 4.56ISDD128 pKa = 3.82DD129 pKa = 4.72QEE131 pKa = 4.25FQSSEE136 pKa = 4.09FVGDD140 pKa = 5.01DD141 pKa = 3.48LDD143 pKa = 3.95EE144 pKa = 4.68LKK146 pKa = 9.83EE147 pKa = 4.19TIEE150 pKa = 4.17WISNDD155 pKa = 2.95KK156 pKa = 10.38HH157 pKa = 7.2LHH159 pKa = 5.67IEE161 pKa = 4.14DD162 pKa = 4.27EE163 pKa = 4.24EE164 pKa = 5.11RR165 pKa = 11.84DD166 pKa = 3.5QYY168 pKa = 11.5SIHH171 pKa = 6.55LRR173 pKa = 11.84LGRR176 pKa = 11.84DD177 pKa = 2.95IEE179 pKa = 4.4TGDD182 pKa = 3.39GVLYY186 pKa = 10.32RR187 pKa = 11.84HH188 pKa = 6.98TIMKK192 pKa = 9.93HH193 pKa = 5.12DD194 pKa = 4.11EE195 pKa = 3.97EE196 pKa = 7.23DD197 pKa = 3.94IEE199 pKa = 5.57DD200 pKa = 3.56EE201 pKa = 4.13MIFYY205 pKa = 10.52FKK207 pKa = 10.47EE208 pKa = 4.17DD209 pKa = 3.23EE210 pKa = 4.25AGYY213 pKa = 9.99IAEE216 pKa = 4.97LFNEE220 pKa = 4.65FTGQNHH226 pKa = 6.22INHH229 pKa = 7.16

Molecular weight: 27.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M0QBR3|A0A6M0QBR3_9BACI Uncharacterized protein OS=Bacillus mesophilus OX=1808955 GN=G4D63_18835 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.59RR3 pKa = 11.84TFQPNKK9 pKa = 8.24RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.7VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.8VLSAA44 pKa = 4.11

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4152

0

4152

1182166

20

1681

284.7

32.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.331 ± 0.041

0.678 ± 0.01

4.974 ± 0.03

7.652 ± 0.04

4.63 ± 0.034

6.756 ± 0.044

2.133 ± 0.018

8.138 ± 0.041

6.721 ± 0.035

10.055 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.649 ± 0.019

4.48 ± 0.028

3.557 ± 0.024

3.824 ± 0.027

3.865 ± 0.029

6.263 ± 0.031

5.53 ± 0.029

7.159 ± 0.031

0.997 ± 0.014

3.608 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski