Firmicutes bacterium CAG:145

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1960 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6END9|R6END9_9FIRM Glycosyltransferase group 1 family protein OS=Firmicutes bacterium CAG:145 OX=1263005 GN=BN497_01522 PE=4 SV=1
MM1 pKa = 7.24NRR3 pKa = 11.84EE4 pKa = 3.7QLYY7 pKa = 10.21EE8 pKa = 4.04YY9 pKa = 11.06LDD11 pKa = 3.72IDD13 pKa = 3.85TPADD17 pKa = 3.44FEE19 pKa = 4.59YY20 pKa = 10.43FEE22 pKa = 4.94NMAALLEE29 pKa = 4.35CEE31 pKa = 3.67EE32 pKa = 5.19DD33 pKa = 3.2IPYY36 pKa = 10.94EE37 pKa = 4.08EE38 pKa = 4.25VCALIEE44 pKa = 5.75DD45 pKa = 3.84VDD47 pKa = 4.04KK48 pKa = 11.74DD49 pKa = 3.98NLAMLIDD56 pKa = 4.0NYY58 pKa = 10.73FEE60 pKa = 4.33EE61 pKa = 5.98LSDD64 pKa = 4.32FYY66 pKa = 11.02PDD68 pKa = 4.24GDD70 pKa = 4.1DD71 pKa = 3.47EE72 pKa = 6.63FYY74 pKa = 11.09MLMDD78 pKa = 5.6KK79 pKa = 10.54IRR81 pKa = 11.84LSLSGMAKK89 pKa = 10.34NSGEE93 pKa = 4.31KK94 pKa = 9.09NTMASLAEE102 pKa = 3.97EE103 pKa = 4.5LCRR106 pKa = 11.84FRR108 pKa = 11.84KK109 pKa = 9.13WYY111 pKa = 9.95SLEE114 pKa = 4.15SEE116 pKa = 4.81VVCVSVSEE124 pKa = 4.3GEE126 pKa = 4.15EE127 pKa = 3.78RR128 pKa = 11.84AEE130 pKa = 4.13TLRR133 pKa = 11.84DD134 pKa = 3.46ALVLSRR140 pKa = 11.84LEE142 pKa = 4.09KK143 pKa = 10.64LDD145 pKa = 3.61GEE147 pKa = 4.25KK148 pKa = 10.24FYY150 pKa = 11.52YY151 pKa = 10.5DD152 pKa = 4.0FSSCKK157 pKa = 10.15GYY159 pKa = 10.84EE160 pKa = 3.62IDD162 pKa = 4.9DD163 pKa = 3.93YY164 pKa = 11.28MMSFADD170 pKa = 4.35VIAACEE176 pKa = 4.09EE177 pKa = 4.39DD178 pKa = 5.79DD179 pKa = 4.48EE180 pKa = 4.93TPQQ183 pKa = 4.18

Molecular weight: 21.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6EZZ6|R6EZZ6_9FIRM Branched-chain amino acid ABC transporter permease protein OS=Firmicutes bacterium CAG:145 OX=1263005 GN=BN497_00359 PE=4 SV=1
MM1 pKa = 7.83AKK3 pKa = 9.58TSLKK7 pKa = 10.48VKK9 pKa = 7.55QQRR12 pKa = 11.84KK13 pKa = 7.82PKK15 pKa = 9.16YY16 pKa = 7.19STRR19 pKa = 11.84AYY21 pKa = 8.48TRR23 pKa = 11.84CRR25 pKa = 11.84LCGRR29 pKa = 11.84PHH31 pKa = 6.58AVLRR35 pKa = 11.84KK36 pKa = 9.63YY37 pKa = 9.83GICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.81LAYY49 pKa = 10.0KK50 pKa = 10.86GEE52 pKa = 4.16IPGVRR57 pKa = 11.84KK58 pKa = 10.13ASWW61 pKa = 2.78

Molecular weight: 7.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1960

0

1960

592543

29

1997

302.3

33.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.962 ± 0.053

1.517 ± 0.027

5.939 ± 0.045

7.52 ± 0.058

4.11 ± 0.033

7.67 ± 0.054

1.489 ± 0.022

8.005 ± 0.047

6.972 ± 0.043

8.524 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.262 ± 0.029

3.902 ± 0.03

3.292 ± 0.025

2.695 ± 0.027

4.617 ± 0.046

5.996 ± 0.053

5.147 ± 0.037

6.817 ± 0.044

0.785 ± 0.017

3.776 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski