Cloacibacillus evryensis DSM 19522

Taxonomy: cellular organisms; Bacteria; Synergistetes; Synergistia; Synergistales; Synergistaceae; Cloacibacillus; Cloacibacillus evryensis

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1082 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A010ZNZ1|A0A010ZNZ1_9BACT L-seryl-tRNA(Sec) selenium transferase OS=Cloacibacillus evryensis DSM 19522 OX=866499 GN=selA PE=3 SV=1
MM1 pKa = 7.58GIKK4 pKa = 10.38VNLDD8 pKa = 3.26DD9 pKa = 6.49CIGCGVCFEE18 pKa = 5.2LCPQNFKK25 pKa = 10.91LDD27 pKa = 3.85EE28 pKa = 4.72DD29 pKa = 3.87EE30 pKa = 4.61GKK32 pKa = 11.14CMVISNEE39 pKa = 4.08VNSSAKK45 pKa = 9.65EE46 pKa = 3.83AADD49 pKa = 3.68SCPVSAITIDD59 pKa = 3.17

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A010YSF8|A0A010YSF8_9BACT Glutamate decarboxylase OS=Cloacibacillus evryensis DSM 19522 OX=866499 GN=Cloev_0173 PE=3 SV=1
MM1 pKa = 7.29TGAAEE6 pKa = 4.05LSEE9 pKa = 3.88HH10 pKa = 6.38RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84LFVKK17 pKa = 10.33RR18 pKa = 11.84LRR20 pKa = 11.84LAALLSLLLLIAALWRR36 pKa = 11.84LGAGEE41 pKa = 4.12WDD43 pKa = 3.11IPAARR48 pKa = 11.84VAALLNPFLDD58 pKa = 4.06EE59 pKa = 4.42ASRR62 pKa = 11.84ASPEE66 pKa = 3.91ALVVRR71 pKa = 11.84SVRR74 pKa = 11.84LPRR77 pKa = 11.84FLAAVGTGGLLAVAGAVLQGLLTNPLAEE105 pKa = 5.16PYY107 pKa = 9.64TLGIAAGAAFGGALGFFFGSFAVTPLAFAGALASLWLVSAIAARR151 pKa = 11.84SGGGAAYY158 pKa = 9.32IVLAGIITNAILSAGVTFLKK178 pKa = 10.84AIADD182 pKa = 3.96DD183 pKa = 3.86RR184 pKa = 11.84LSAIVLWLMGSLSGASPLAALSVWGAAFILFVPAFIYY221 pKa = 10.4ARR223 pKa = 11.84QIDD226 pKa = 4.45AVSLSEE232 pKa = 4.18GQGALLGIDD241 pKa = 3.76EE242 pKa = 4.69KK243 pKa = 11.24KK244 pKa = 10.76LRR246 pKa = 11.84ALLLAVSSLATAAAVSFFGIIGFVGLVVPHH276 pKa = 6.75LMRR279 pKa = 11.84SLTGPATRR287 pKa = 11.84PLLIFSFLGGGLLLALADD305 pKa = 4.01GAAQRR310 pKa = 11.84LGEE313 pKa = 4.38LPVGVITALIGGPFFCWILMSRR335 pKa = 11.84RR336 pKa = 11.84RR337 pKa = 11.84GAA339 pKa = 3.09

Molecular weight: 34.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1082

0

1082

361961

30

4346

334.5

36.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.404 ± 0.111

1.551 ± 0.038

5.153 ± 0.06

6.72 ± 0.092

4.113 ± 0.064

8.488 ± 0.072

1.569 ± 0.032

6.478 ± 0.059

5.478 ± 0.066

9.273 ± 0.09

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.995 ± 0.043

3.332 ± 0.048

4.188 ± 0.037

2.332 ± 0.043

5.639 ± 0.079

5.939 ± 0.063

4.999 ± 0.065

7.097 ± 0.071

1.084 ± 0.022

3.168 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski