Pectobacterium phage PPWS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Corkvirinae; Kotilavirus; Pectobacterium virus PPWS1

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0UWN1|A0A0P0UWN1_9CAUD Endolysin OS=Pectobacterium phage PPWS1 OX=1685500 GN=56 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.29ISIAEE7 pKa = 4.15PNLSNHH13 pKa = 6.53LEE15 pKa = 4.2GFTARR20 pKa = 11.84VATEE24 pKa = 3.86TRR26 pKa = 11.84SKK28 pKa = 10.76LRR30 pKa = 11.84EE31 pKa = 4.12LFCDD35 pKa = 3.54KK36 pKa = 10.18PASFGVPKK44 pKa = 10.53ALSKK48 pKa = 11.54DD49 pKa = 3.28EE50 pKa = 4.08LTSIKK55 pKa = 10.48QEE57 pKa = 4.0VYY59 pKa = 10.77SRR61 pKa = 11.84ADD63 pKa = 3.1THH65 pKa = 6.16IARR68 pKa = 11.84AVGLLLPPDD77 pKa = 3.58VTIPSAEE84 pKa = 4.08LTDD87 pKa = 3.88DD88 pKa = 5.54ARR90 pKa = 11.84IEE92 pKa = 3.95WMLRR96 pKa = 11.84ALIAAGFAAYY106 pKa = 8.93MRR108 pKa = 11.84EE109 pKa = 4.03AVQDD113 pKa = 3.78YY114 pKa = 10.61SPVLHH119 pKa = 6.08SHH121 pKa = 4.99YY122 pKa = 10.81LEE124 pKa = 4.74YY125 pKa = 11.15ANSWLKK131 pKa = 11.03VFLFKK136 pKa = 11.38NNLPLPRR143 pKa = 11.84SSDD146 pKa = 3.7VASHH150 pKa = 5.52VAVGVAAAPSGTCGYY165 pKa = 10.03IDD167 pKa = 4.18KK168 pKa = 10.8LYY170 pKa = 11.01LRR172 pKa = 11.84LARR175 pKa = 11.84PLVEE179 pKa = 5.74GYY181 pKa = 9.59PPYY184 pKa = 10.42QGLFFQGPTLPPALYY199 pKa = 10.76SLVEE203 pKa = 4.29TNTTAVLATLRR214 pKa = 11.84AEE216 pKa = 4.51MGDD219 pKa = 3.68SPRR222 pKa = 11.84WAVEE226 pKa = 3.43TRR228 pKa = 11.84NFLRR232 pKa = 11.84GVVYY236 pKa = 10.04TEE238 pKa = 4.76AEE240 pKa = 4.01VAMFAGYY247 pKa = 7.8VTLEE251 pKa = 4.13DD252 pKa = 4.63FYY254 pKa = 11.53KK255 pKa = 10.77LNYY258 pKa = 9.39RR259 pKa = 11.84RR260 pKa = 11.84IPLARR265 pKa = 11.84AIKK268 pKa = 9.88EE269 pKa = 3.81ITGSDD274 pKa = 3.13TAAQKK279 pKa = 10.73AADD282 pKa = 3.8VRR284 pKa = 11.84DD285 pKa = 3.69VTSYY289 pKa = 11.43DD290 pKa = 3.29IVLHH294 pKa = 6.14PNDD297 pKa = 3.91RR298 pKa = 11.84PFGAEE303 pKa = 3.7YY304 pKa = 10.97VRR306 pKa = 11.84MRR308 pKa = 11.84EE309 pKa = 4.24EE310 pKa = 3.57NSEE313 pKa = 4.11LHH315 pKa = 6.59SCMTSPAYY323 pKa = 10.04AYY325 pKa = 9.43SSPYY329 pKa = 9.6GVHH332 pKa = 6.44PCDD335 pKa = 3.8VYY337 pKa = 10.19STAYY341 pKa = 9.98YY342 pKa = 10.06GQGDD346 pKa = 3.74NGLVLVEE353 pKa = 4.26ATQFGIPVGRR363 pKa = 11.84GILNVYY369 pKa = 10.39SNTIVRR375 pKa = 11.84WYY377 pKa = 10.05GAYY380 pKa = 9.54KK381 pKa = 10.26AIVMLGNLYY390 pKa = 10.23NIHH393 pKa = 6.29VDD395 pKa = 3.28ADD397 pKa = 3.74SLDD400 pKa = 3.72GVRR403 pKa = 11.84LALIQDD409 pKa = 3.8GDD411 pKa = 5.03LITAPYY417 pKa = 10.54LDD419 pKa = 3.7GCQRR423 pKa = 11.84VEE425 pKa = 4.36LDD427 pKa = 3.84DD428 pKa = 4.32GVLIVDD434 pKa = 3.64ARR436 pKa = 11.84GSIEE440 pKa = 3.89MDD442 pKa = 3.23DD443 pKa = 3.35TSGYY447 pKa = 10.45QEE449 pKa = 3.8TDD451 pKa = 2.86EE452 pKa = 4.19EE453 pKa = 4.39DD454 pKa = 4.02RR455 pKa = 11.84YY456 pKa = 10.99EE457 pKa = 4.08CAISGDD463 pKa = 4.15YY464 pKa = 10.79YY465 pKa = 10.49PEE467 pKa = 4.82SEE469 pKa = 5.19LRR471 pKa = 11.84YY472 pKa = 10.09QSTTDD477 pKa = 2.89TYY479 pKa = 11.57YY480 pKa = 11.3NPDD483 pKa = 3.39NTGMNHH489 pKa = 7.22AEE491 pKa = 4.12HH492 pKa = 6.97CPVLDD497 pKa = 3.61EE498 pKa = 3.85WHH500 pKa = 7.11DD501 pKa = 3.65RR502 pKa = 11.84HH503 pKa = 6.51SGHH506 pKa = 6.92YY507 pKa = 9.82IRR509 pKa = 11.84IDD511 pKa = 3.68GEE513 pKa = 4.39QTWVHH518 pKa = 6.72DD519 pKa = 4.13SVTSDD524 pKa = 3.24EE525 pKa = 5.31LADD528 pKa = 4.65DD529 pKa = 4.59DD530 pKa = 4.75CTYY533 pKa = 10.75MDD535 pKa = 4.17DD536 pKa = 3.94TIGYY540 pKa = 6.44TRR542 pKa = 11.84SPDD545 pKa = 3.43DD546 pKa = 3.97YY547 pKa = 11.65VWDD550 pKa = 4.38DD551 pKa = 3.59VNEE554 pKa = 4.02QYY556 pKa = 9.65YY557 pKa = 8.43TVSDD561 pKa = 3.86YY562 pKa = 11.38DD563 pKa = 3.51YY564 pKa = 10.97LIAEE568 pKa = 4.69RR569 pKa = 11.84EE570 pKa = 4.07QEE572 pKa = 4.0EE573 pKa = 4.79SNEE576 pKa = 4.25SNQQ579 pKa = 3.71

Molecular weight: 65.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0UW59|A0A0P0UW59_9CAUD Uncharacterized protein OS=Pectobacterium phage PPWS1 OX=1685500 PE=4 SV=1
MM1 pKa = 7.98EE2 pKa = 4.93IRR4 pKa = 11.84DD5 pKa = 3.99VIAYY9 pKa = 8.92DD10 pKa = 3.52PTSPTGLRR18 pKa = 11.84WLVATSNRR26 pKa = 11.84VKK28 pKa = 10.67VGSPAIRR35 pKa = 11.84AKK37 pKa = 10.75HH38 pKa = 5.22SGGYY42 pKa = 8.27YY43 pKa = 10.08SGMVLGACLEE53 pKa = 4.07PRR55 pKa = 11.84HH56 pKa = 5.68RR57 pKa = 11.84ARR59 pKa = 11.84RR60 pKa = 11.84SGG62 pKa = 3.19

Molecular weight: 6.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13783

43

1230

250.6

27.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.671 ± 0.366

1.081 ± 0.173

6.392 ± 0.212

5.144 ± 0.304

2.996 ± 0.159

7.596 ± 0.259

2.22 ± 0.197

4.629 ± 0.166

4.041 ± 0.303

8.779 ± 0.234

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.822 ± 0.171

4.194 ± 0.247

4.099 ± 0.228

4.484 ± 0.396

5.978 ± 0.289

6.515 ± 0.294

6.624 ± 0.485

7.589 ± 0.237

1.291 ± 0.134

3.853 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski