Saccharospirillum sp. MSK14-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Saccharospirillaceae; Saccharospirillum; unclassified Saccharospirillum

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3452 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T6E7G7|A0A2T6E7G7_9GAMM Probable membrane transporter protein OS=Saccharospirillum sp. MSK14-1 OX=1897632 GN=BGP77_02690 PE=3 SV=1
MM1 pKa = 7.86TDD3 pKa = 3.88LDD5 pKa = 4.77LDD7 pKa = 3.92QLADD11 pKa = 3.58WLEE14 pKa = 4.08DD15 pKa = 3.57DD16 pKa = 5.67QRR18 pKa = 11.84ADD20 pKa = 3.5DD21 pKa = 4.79CLDD24 pKa = 2.8IHH26 pKa = 6.65GLHH29 pKa = 7.13GYY31 pKa = 7.98LTALAISAQPLSDD44 pKa = 3.47EE45 pKa = 4.33WLASAIDD52 pKa = 3.81QSLHH56 pKa = 6.7DD57 pKa = 5.15LPDD60 pKa = 4.92AEE62 pKa = 4.75AQWFADD68 pKa = 3.18QCVQLHH74 pKa = 4.61QQIATEE80 pKa = 5.01LYY82 pKa = 9.99ADD84 pKa = 4.82DD85 pKa = 6.08GIALTFEE92 pKa = 4.59PTADD96 pKa = 3.52WQDD99 pKa = 3.39SPQQFWAEE107 pKa = 3.55AFMEE111 pKa = 4.81VVFTEE116 pKa = 4.54PEE118 pKa = 4.03HH119 pKa = 6.07FASDD123 pKa = 3.97QEE125 pKa = 4.55DD126 pKa = 3.76SLATLLLPIEE136 pKa = 4.38VASGLFADD144 pKa = 4.41EE145 pKa = 5.18ADD147 pKa = 3.63YY148 pKa = 11.28QPIYY152 pKa = 9.9RR153 pKa = 11.84QPKK156 pKa = 9.69LIRR159 pKa = 11.84QMVDD163 pKa = 3.19DD164 pKa = 4.85LPEE167 pKa = 4.19VLTDD171 pKa = 5.73LYY173 pKa = 11.48LLLQAPVKK181 pKa = 10.62

Molecular weight: 20.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T6E9N9|A0A2T6E9N9_9GAMM Uncharacterized protein OS=Saccharospirillum sp. MSK14-1 OX=1897632 GN=BGP77_16620 PE=4 SV=1
MM1 pKa = 7.75PNASSPAVISRR12 pKa = 11.84PLAITILMLAASFFAANHH30 pKa = 4.88VSARR34 pKa = 11.84LAFDD38 pKa = 3.64HH39 pKa = 6.54GTGLLLAILTRR50 pKa = 11.84GTMALLIMTSLALWQRR66 pKa = 11.84HH67 pKa = 5.04AFRR70 pKa = 11.84VPAGKK75 pKa = 10.23RR76 pKa = 11.84GWQILLGLMITGQSLCLYY94 pKa = 9.17SAVARR99 pKa = 11.84VPVAVALLMVNTWPILLALLNWVVSGKK126 pKa = 10.05RR127 pKa = 11.84PSGRR131 pKa = 11.84LVLIMTVILIGVVLVLDD148 pKa = 4.1VPAWLSDD155 pKa = 3.57PTAMGPDD162 pKa = 3.9WLPGVCFALAAACFFTVAIWTTDD185 pKa = 2.98NKK187 pKa = 10.35LAGMPGAVRR196 pKa = 11.84SVFTMTTVLVVMVVGGVFNLVPGGLDD222 pKa = 4.16LPATSQGWIGLALLAVFYY240 pKa = 8.79GTGSTVLFVLVPRR253 pKa = 11.84LDD255 pKa = 3.55MARR258 pKa = 11.84NAPVMNFEE266 pKa = 4.51PVASLILGFLVLGQMLSLWQLIGGAIVLSGIIWLSLARR304 pKa = 11.84HH305 pKa = 5.76

Molecular weight: 32.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3452

0

3452

1122755

39

3612

325.2

35.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.227 ± 0.046

0.838 ± 0.012

6.27 ± 0.038

5.923 ± 0.05

3.759 ± 0.027

7.463 ± 0.046

2.255 ± 0.021

5.096 ± 0.032

2.99 ± 0.039

11.378 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.021

3.399 ± 0.033

4.478 ± 0.03

4.754 ± 0.034

6.266 ± 0.046

5.993 ± 0.037

5.229 ± 0.042

7.089 ± 0.038

1.573 ± 0.019

2.664 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski