Escherichia phage HX01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Mosigvirus; Escherichia virus HX01

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0G0F5|K0G0F5_9CAUD Uncharacterized protein OS=Escherichia phage HX01 OX=1237364 GN=HX01_0137 PE=4 SV=1
MM1 pKa = 7.55TIDD4 pKa = 3.12ISNYY8 pKa = 9.52LEE10 pKa = 4.1EE11 pKa = 5.18PFDD14 pKa = 3.55EE15 pKa = 5.43AIPYY19 pKa = 9.18LVKK22 pKa = 10.9LLGRR26 pKa = 11.84EE27 pKa = 3.92FKK29 pKa = 10.8INFDD33 pKa = 3.59IDD35 pKa = 3.71PLNPHH40 pKa = 6.83DD41 pKa = 4.57VSFSINGTPVEE52 pKa = 4.16YY53 pKa = 10.13EE54 pKa = 3.79FCIEE58 pKa = 3.8EE59 pKa = 4.27DD60 pKa = 3.24GRR62 pKa = 11.84FYY64 pKa = 11.14FNLMRR69 pKa = 5.88

Molecular weight: 8.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0G7T1|K0G7T1_9CAUD Uncharacterized protein OS=Escherichia phage HX01 OX=1237364 GN=HX01_0247 PE=4 SV=1
MM1 pKa = 7.73AKK3 pKa = 9.38QAKK6 pKa = 9.05AKK8 pKa = 10.23KK9 pKa = 9.55AVEE12 pKa = 4.2KK13 pKa = 10.87KK14 pKa = 10.61VDD16 pKa = 3.8GTSKK20 pKa = 9.78RR21 pKa = 11.84AGYY24 pKa = 10.0KK25 pKa = 9.69RR26 pKa = 11.84GSNSRR31 pKa = 11.84INQTVEE37 pKa = 3.65KK38 pKa = 9.9IMRR41 pKa = 11.84RR42 pKa = 11.84ARR44 pKa = 11.84AVLRR48 pKa = 11.84DD49 pKa = 3.37DD50 pKa = 3.75ASRR53 pKa = 11.84FGKK56 pKa = 10.22VVAA59 pKa = 4.81

Molecular weight: 6.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

4698

36

134

75.8

8.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.577 ± 0.361

1.341 ± 0.177

6.109 ± 0.415

9.068 ± 0.429

4.853 ± 0.36

4.981 ± 0.261

1.809 ± 0.158

7.386 ± 0.493

8.812 ± 0.512

8.003 ± 0.419

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.342 ± 0.217

5.258 ± 0.323

2.767 ± 0.306

3.257 ± 0.275

4.491 ± 0.365

5.236 ± 0.293

4.853 ± 0.31

7.195 ± 0.405

1.405 ± 0.18

4.257 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski