Botryotinia fuckeliana (strain BcDW1) (Noble rot fungus) (Botrytis cinerea)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Sclerotiniaceae; Botrytis; Botrytis cinerea

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11022 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M7TFC0|M7TFC0_BOTF1 Putative zinc metalloproteinase protein OS=Botryotinia fuckeliana (strain BcDW1) OX=1290391 GN=BcDW1_9222 PE=3 SV=1
MM1 pKa = 7.38PTNLDD6 pKa = 3.27GLSFVDD12 pKa = 6.06FEE14 pKa = 5.38LFDD17 pKa = 4.04SQSQEE22 pKa = 4.08YY23 pKa = 10.01EE24 pKa = 3.98NSDD27 pKa = 4.17DD28 pKa = 3.71PWLEE32 pKa = 3.58PSISIGSPKK41 pKa = 10.38LHH43 pKa = 7.11LNDD46 pKa = 4.11CQSCKK51 pKa = 9.79TFNTFDD57 pKa = 3.66TPRR60 pKa = 11.84SSEE63 pKa = 3.89MSLSQVDD70 pKa = 4.3LCSPPPNSATSSWSMGNEE88 pKa = 3.79SMVLFPTIYY97 pKa = 10.57SGDD100 pKa = 3.62YY101 pKa = 10.69LGASNSDD108 pKa = 3.46FVVGVGQWMCGTMVEE123 pKa = 4.63ANDD126 pKa = 5.03LEE128 pKa = 4.7DD129 pKa = 3.45EE130 pKa = 4.52SKK132 pKa = 10.65ILRR135 pKa = 11.84NTDD138 pKa = 3.03NNTTSQPSQATGRR151 pKa = 11.84ITDD154 pKa = 3.52SFEE157 pKa = 5.11LIFYY161 pKa = 10.63GDD163 pKa = 3.52EE164 pKa = 3.96

Molecular weight: 18.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M7U514|M7U514_BOTF1 Putative non-ribosomal peptide synthetase protein OS=Botryotinia fuckeliana (strain BcDW1) OX=1290391 GN=BcDW1_9601 PE=4 SV=1
MM1 pKa = 7.33SPRR4 pKa = 11.84YY5 pKa = 9.91AKK7 pKa = 10.46DD8 pKa = 3.71AIPAFSCHH16 pKa = 6.63LVPLSPPRR24 pKa = 11.84LAFQSYY30 pKa = 8.9RR31 pKa = 11.84CRR33 pKa = 11.84TSCIGKK39 pKa = 9.72KK40 pKa = 10.04FEE42 pKa = 4.03LTLRR46 pKa = 11.84SACTIEE52 pKa = 4.12IVPSRR57 pKa = 11.84FLMPKK62 pKa = 9.86RR63 pKa = 11.84VRR65 pKa = 11.84LLHH68 pKa = 6.17PVLL71 pKa = 4.87

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11022

0

11022

5548963

66

6903

503.4

55.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.802 ± 0.022

1.129 ± 0.008

5.605 ± 0.014

6.619 ± 0.025

3.703 ± 0.015

6.817 ± 0.021

2.247 ± 0.011

5.453 ± 0.016

5.472 ± 0.022

8.504 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.008

4.27 ± 0.013

5.774 ± 0.023

3.909 ± 0.015

5.594 ± 0.024

8.79 ± 0.031

6.146 ± 0.02

5.768 ± 0.015

1.379 ± 0.009

2.805 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski