Clostridium sp. CAG:568

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1211 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5ZKK2|R5ZKK2_9CLOT UDP-galactopyranose mutase OS=Clostridium sp. CAG:568 OX=1262821 GN=BN713_00528 PE=3 SV=1
MM1 pKa = 7.13QSKK4 pKa = 10.1FKK6 pKa = 11.07LLTLVLLSTTFFLTSCGDD24 pKa = 3.55STSNPTTPSNQVTDD38 pKa = 3.35NSTNKK43 pKa = 9.82PSAPSVNTDD52 pKa = 3.09KK53 pKa = 11.34KK54 pKa = 10.84DD55 pKa = 3.31TTTSSNKK62 pKa = 9.5KK63 pKa = 10.13DD64 pKa = 3.74DD65 pKa = 4.95ASSDD69 pKa = 2.95WYY71 pKa = 10.84ASEE74 pKa = 4.66KK75 pKa = 10.84EE76 pKa = 3.91IMKK79 pKa = 10.2QAFGFDD85 pKa = 3.52FAIPFADD92 pKa = 3.4GLTDD96 pKa = 3.38NRR98 pKa = 11.84EE99 pKa = 4.0SSLEE103 pKa = 3.94KK104 pKa = 10.46DD105 pKa = 3.16DD106 pKa = 4.79SGNFFYY112 pKa = 10.14THH114 pKa = 7.65DD115 pKa = 4.16MDD117 pKa = 5.6CGNLTSAYY125 pKa = 8.9KK126 pKa = 10.6SKK128 pKa = 11.3VEE130 pKa = 4.02NEE132 pKa = 4.01EE133 pKa = 3.67YY134 pKa = 10.13TYY136 pKa = 11.47DD137 pKa = 3.85STQDD141 pKa = 3.45GYY143 pKa = 11.63DD144 pKa = 3.72LYY146 pKa = 11.1AYY148 pKa = 10.47SYY150 pKa = 11.47DD151 pKa = 3.63DD152 pKa = 3.35TNLIVLQIGYY162 pKa = 9.77SDD164 pKa = 4.6DD165 pKa = 3.88YY166 pKa = 11.73GFEE169 pKa = 3.82IYY171 pKa = 10.33SWVEE175 pKa = 3.88SNTDD179 pKa = 3.54SNVTEE184 pKa = 4.31YY185 pKa = 11.47SSFPYY190 pKa = 10.73ADD192 pKa = 2.85IATFFNLTSVTNNQIPSFDD211 pKa = 3.46IASDD215 pKa = 3.37EE216 pKa = 4.36SYY218 pKa = 11.33YY219 pKa = 11.02GYY221 pKa = 9.39TSKK224 pKa = 10.94SEE226 pKa = 4.04SSSEE230 pKa = 3.69FVVYY234 pKa = 8.14GTIDD238 pKa = 3.24SSITDD243 pKa = 3.31SQMDD247 pKa = 3.9TNYY250 pKa = 11.05SNALKK255 pKa = 10.68ALGYY259 pKa = 8.75TVTVQDD265 pKa = 3.89GYY267 pKa = 11.3YY268 pKa = 9.44PYY270 pKa = 11.13AEE272 pKa = 4.23SEE274 pKa = 4.31EE275 pKa = 4.36KK276 pKa = 10.97GFLLIFGCDD285 pKa = 2.93NSVFFLDD292 pKa = 3.08IMKK295 pKa = 10.4YY296 pKa = 10.1EE297 pKa = 4.33SSTTDD302 pKa = 3.47PDD304 pKa = 4.12TPSTPDD310 pKa = 3.26TTIKK314 pKa = 10.12TITIDD319 pKa = 4.33ANNFDD324 pKa = 3.47AKK326 pKa = 11.1YY327 pKa = 10.01PGFEE331 pKa = 4.17SDD333 pKa = 3.92LTISGYY339 pKa = 7.28TFKK342 pKa = 10.92YY343 pKa = 10.3DD344 pKa = 4.06YY345 pKa = 11.4IMNSNDD351 pKa = 3.25KK352 pKa = 10.52IQFRR356 pKa = 11.84NLSKK360 pKa = 10.99SNSYY364 pKa = 10.56LFNSTAMNLSTVVLNVDD381 pKa = 3.44VTTKK385 pKa = 10.65GDD387 pKa = 3.69YY388 pKa = 10.38VGYY391 pKa = 8.91PSLYY395 pKa = 9.46ASSSILSADD404 pKa = 3.48NNGAEE409 pKa = 4.43IKK411 pKa = 10.45PVITDD416 pKa = 3.35KK417 pKa = 8.88TTYY420 pKa = 10.0TYY422 pKa = 8.94TIPEE426 pKa = 4.3GFSFFKK432 pKa = 10.41VTGHH436 pKa = 6.54KK437 pKa = 10.43NYY439 pKa = 10.88AVLLYY444 pKa = 11.3SMTINLKK451 pKa = 10.28

Molecular weight: 50.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5ZTC5|R5ZTC5_9CLOT HATPase_c_5 domain-containing protein OS=Clostridium sp. CAG:568 OX=1262821 GN=BN713_01102 PE=4 SV=1
MM1 pKa = 7.53AVPTRR6 pKa = 11.84RR7 pKa = 11.84ASKK10 pKa = 7.97TRR12 pKa = 11.84KK13 pKa = 8.94RR14 pKa = 11.84LRR16 pKa = 11.84RR17 pKa = 11.84THH19 pKa = 6.21FHH21 pKa = 6.74LAEE24 pKa = 4.1QNLTRR29 pKa = 11.84CSNCGAMIQSHH40 pKa = 7.06RR41 pKa = 11.84ICSSCGFYY49 pKa = 10.23RR50 pKa = 11.84GKK52 pKa = 10.49KK53 pKa = 9.08VLDD56 pKa = 3.58VKK58 pKa = 11.12VKK60 pKa = 10.58EE61 pKa = 4.34DD62 pKa = 3.19

Molecular weight: 7.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1211

0

1211

438710

30

1570

362.3

40.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.659 ± 0.068

1.177 ± 0.022

6.751 ± 0.054

6.346 ± 0.066

4.863 ± 0.056

6.292 ± 0.056

1.439 ± 0.026

7.921 ± 0.075

8.649 ± 0.073

8.896 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.472 ± 0.032

5.939 ± 0.053

3.087 ± 0.041

2.553 ± 0.033

3.348 ± 0.05

7.413 ± 0.074

5.571 ± 0.081

6.229 ± 0.051

0.798 ± 0.023

4.593 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski