Bacillus circulans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4949 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J1LFX0|A0A0J1LFX0_BACCI Methyltransferase type 12 OS=Bacillus circulans OX=1397 GN=ABW02_03785 PE=4 SV=1
MM1 pKa = 7.72EE2 pKa = 4.34KK3 pKa = 10.31QGWVSVWLGNINDD16 pKa = 3.78EE17 pKa = 4.34DD18 pKa = 4.69SIGEE22 pKa = 4.28YY23 pKa = 10.77VDD25 pKa = 3.41LTYY28 pKa = 11.2DD29 pKa = 3.5EE30 pKa = 5.94DD31 pKa = 4.97GEE33 pKa = 4.74SVPSQFFIDD42 pKa = 4.24FKK44 pKa = 11.04IDD46 pKa = 3.26VDD48 pKa = 3.98EE49 pKa = 4.49TDD51 pKa = 3.34EE52 pKa = 4.02DD53 pKa = 4.45TIEE56 pKa = 4.55KK57 pKa = 9.87EE58 pKa = 4.24VYY60 pKa = 9.79KK61 pKa = 10.98NSSSDD66 pKa = 2.97IATLLDD72 pKa = 3.63GCSYY76 pKa = 11.1EE77 pKa = 4.62EE78 pKa = 3.8IVIPKK83 pKa = 8.43IQKK86 pKa = 10.09SINLKK91 pKa = 9.63KK92 pKa = 10.62SYY94 pKa = 10.56NAVILIYY101 pKa = 10.54NFQYY105 pKa = 10.79QNEE108 pKa = 4.2ISSTGAFDD116 pKa = 6.33FITATNYY123 pKa = 8.28EE124 pKa = 4.13

Molecular weight: 14.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J1HLW7|A0A0J1HLW7_BACCI Integrase OS=Bacillus circulans OX=1397 GN=ABW02_25905 PE=4 SV=1
TT1 pKa = 6.97SANALIVKK9 pKa = 7.61NTSANARR16 pKa = 11.84IAGKK20 pKa = 8.64TSANALIVKK29 pKa = 7.61NTSANARR36 pKa = 11.84IAGKK40 pKa = 8.64TSANALIVKK49 pKa = 7.61NTSANARR56 pKa = 11.84IAGKK60 pKa = 8.64TSANALIVKK69 pKa = 7.61NTSANARR76 pKa = 11.84IAGKK80 pKa = 8.64TSANALIVKK89 pKa = 7.61NTSANARR96 pKa = 11.84IAGKK100 pKa = 8.64TSANALIVKK109 pKa = 7.61NTSANARR116 pKa = 11.84IAGKK120 pKa = 8.64TSANALIVKK129 pKa = 7.63NTSANN134 pKa = 3.37

Molecular weight: 13.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4949

0

4949

1429561

26

1517

288.9

32.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.562 ± 0.038

0.724 ± 0.011

4.929 ± 0.027

7.6 ± 0.037

4.64 ± 0.033

6.463 ± 0.038

1.979 ± 0.018

8.573 ± 0.034

7.513 ± 0.035

9.896 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.7 ± 0.014

4.794 ± 0.023

3.391 ± 0.019

3.658 ± 0.022

3.739 ± 0.025

6.115 ± 0.028

5.365 ± 0.024

6.536 ± 0.027

1.053 ± 0.015

3.771 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski