Mycobacterium phage Nibb

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411B5H7|A0A411B5H7_9CAUD Uncharacterized protein OS=Mycobacterium phage Nibb OX=2510585 GN=60 PE=4 SV=1
MM1 pKa = 7.66IDD3 pKa = 4.42ANRR6 pKa = 11.84PWWADD11 pKa = 3.68RR12 pKa = 11.84QAVEE16 pKa = 4.47AYY18 pKa = 10.03VAEE21 pKa = 4.28THH23 pKa = 7.19FDD25 pKa = 3.25ATLDD29 pKa = 3.89YY30 pKa = 11.37LDD32 pKa = 4.44GLAAAVEE39 pKa = 4.16HH40 pKa = 6.11QIAYY44 pKa = 9.98LADD47 pKa = 4.49DD48 pKa = 4.23PAVAASSALAKK59 pKa = 10.3LDD61 pKa = 3.53EE62 pKa = 4.85LFVGDD67 pKa = 3.7RR68 pKa = 11.84LMVGRR73 pKa = 11.84LPFDD77 pKa = 3.8NEE79 pKa = 3.81LQLALTLGADD89 pKa = 3.68GMNVEE94 pKa = 4.89FQTAPRR100 pKa = 11.84PEE102 pKa = 4.41PLPYY106 pKa = 10.03QQGDD110 pKa = 3.59PAHH113 pKa = 6.96VMVCACSHH121 pKa = 7.6PYY123 pKa = 9.07TDD125 pKa = 4.85HH126 pKa = 7.54SDD128 pKa = 3.99VGCSWCAHH136 pKa = 5.95CSGFKK141 pKa = 10.34YY142 pKa = 10.64SHH144 pKa = 7.56DD145 pKa = 4.27DD146 pKa = 3.53DD147 pKa = 5.55GKK149 pKa = 10.19VV150 pKa = 2.86

Molecular weight: 16.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411B5I9|A0A411B5I9_9CAUD Cas4 family endonuclease OS=Mycobacterium phage Nibb OX=2510585 GN=62 PE=4 SV=1
MM1 pKa = 7.19KK2 pKa = 10.27RR3 pKa = 11.84VRR5 pKa = 11.84AYY7 pKa = 9.06RR8 pKa = 11.84APVVEE13 pKa = 4.12VEE15 pKa = 4.02RR16 pKa = 11.84PEE18 pKa = 4.46VIVHH22 pKa = 5.99GKK24 pKa = 8.86VLEE27 pKa = 4.76PGTEE31 pKa = 3.78VSIAGEE37 pKa = 3.73RR38 pKa = 11.84GRR40 pKa = 11.84FRR42 pKa = 11.84FVKK45 pKa = 9.98SARR48 pKa = 11.84ASSGRR53 pKa = 11.84VTCDD57 pKa = 4.2FIGPDD62 pKa = 4.12DD63 pKa = 3.76QTKK66 pKa = 9.74CWRR69 pKa = 11.84SFYY72 pKa = 9.91PEE74 pKa = 4.32RR75 pKa = 11.84IKK77 pKa = 9.98TVHH80 pKa = 6.33RR81 pKa = 11.84LNRR84 pKa = 11.84TRR86 pKa = 11.84ANAAA90 pKa = 3.2

Molecular weight: 10.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

19074

38

1355

187.0

20.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.343 ± 0.464

1.122 ± 0.149

6.433 ± 0.253

5.657 ± 0.334

2.648 ± 0.185

9.107 ± 0.502

2.191 ± 0.159

3.712 ± 0.181

3.209 ± 0.179

8.561 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.228 ± 0.121

2.574 ± 0.18

5.804 ± 0.258

3.062 ± 0.148

6.884 ± 0.38

5.253 ± 0.239

5.667 ± 0.223

8.006 ± 0.26

2.045 ± 0.096

2.496 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski