Bifidobacterium pullorum subsp. gallinarum

Taxonomy: cellular organisms;

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1652 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087AN30|A0A087AN30_9BIFI Glutaredoxin nrdH OS=Bifidobacterium pullorum subsp. gallinarum OX=78344 GN=BIGA_1684 PE=4 SV=1
MM1 pKa = 7.15TGNMGGSGMRR11 pKa = 11.84RR12 pKa = 11.84GVMVVIGVVAVVVSLVAGCAVGVGIAYY39 pKa = 10.58VMGNRR44 pKa = 11.84DD45 pKa = 2.99GHH47 pKa = 7.4ALVADD52 pKa = 4.47SAADD56 pKa = 3.83GAGTSSDD63 pKa = 3.39TGADD67 pKa = 3.18ADD69 pKa = 4.44GRR71 pKa = 11.84DD72 pKa = 3.78DD73 pKa = 4.5AAAGDD78 pKa = 4.33SASGDD83 pKa = 3.23VDD85 pKa = 4.17FEE87 pKa = 4.54SGRR90 pKa = 11.84DD91 pKa = 3.68DD92 pKa = 5.93DD93 pKa = 5.59GDD95 pKa = 4.13AVDD98 pKa = 5.61GDD100 pKa = 4.82DD101 pKa = 4.21SRR103 pKa = 11.84QSASQADD110 pKa = 4.24DD111 pKa = 3.82SDD113 pKa = 4.61HH114 pKa = 6.08CAAWVGMYY122 pKa = 7.65YY123 pKa = 10.14TLGDD127 pKa = 3.3ARR129 pKa = 11.84DD130 pKa = 3.89YY131 pKa = 11.86VDD133 pKa = 5.23LKK135 pKa = 10.83SGCGGSGLMSRR146 pKa = 11.84GMWPKK151 pKa = 9.47LTYY154 pKa = 10.61DD155 pKa = 3.55PGSFVDD161 pKa = 4.18EE162 pKa = 4.55GNGRR166 pKa = 11.84YY167 pKa = 9.28SWTVTSKK174 pKa = 7.88MTYY177 pKa = 10.2DD178 pKa = 3.77PQQTDD183 pKa = 3.93PEE185 pKa = 4.21DD186 pKa = 3.2QAYY189 pKa = 10.75CDD191 pKa = 4.1GQPTTSRR198 pKa = 11.84SFEE201 pKa = 4.33VIPTDD206 pKa = 3.48QSPTGVILHH215 pKa = 6.8DD216 pKa = 3.75TTPGIDD222 pKa = 3.2QTSAQSYY229 pKa = 6.9YY230 pKa = 11.24APDD233 pKa = 4.86GYY235 pKa = 9.6TGGMPQQ241 pKa = 3.59

Molecular weight: 24.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087AN49|A0A087AN49_9BIFI Major facilitator superfamily protein OS=Bifidobacterium pullorum subsp. gallinarum OX=78344 GN=BIGA_1702 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.82MKK15 pKa = 9.64HH16 pKa = 5.7GFRR19 pKa = 11.84LRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.06GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1652

0

1652

621777

37

3126

376.4

41.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.272 ± 0.073

0.991 ± 0.02

6.785 ± 0.043

5.934 ± 0.054

3.343 ± 0.035

8.229 ± 0.056

2.14 ± 0.028

5.199 ± 0.044

3.064 ± 0.047

8.895 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.659 ± 0.025

3.003 ± 0.038

4.803 ± 0.036

3.291 ± 0.033

6.675 ± 0.068

5.556 ± 0.043

5.955 ± 0.042

8.064 ± 0.045

1.396 ± 0.026

2.746 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski