Streptococcus pyogenes phage T12 (Bacteriophage T12)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A097PAT4|A0A097PAT4_BPT12 Uncharacterized protein OS=Streptococcus pyogenes phage T12 OX=35344 GN=T12_06c PE=4 SV=1
MM1 pKa = 7.13ATVSEE6 pKa = 4.8VISYY10 pKa = 8.69WRR12 pKa = 11.84GLADD16 pKa = 3.43TNQGYY21 pKa = 9.1DD22 pKa = 4.45ADD24 pKa = 5.18LAWGWQCADD33 pKa = 3.49VTNGTTTNFFGVTLWGNAIDD53 pKa = 5.23LLDD56 pKa = 3.77SAKK59 pKa = 10.87AQGLEE64 pKa = 4.26VIYY67 pKa = 9.61DD68 pKa = 3.63APGINPKK75 pKa = 10.66AGDD78 pKa = 4.02LFVMYY83 pKa = 8.9TYY85 pKa = 10.8AHH87 pKa = 7.4PYY89 pKa = 7.75GHH91 pKa = 6.73TGIVIADD98 pKa = 3.46SDD100 pKa = 4.66GYY102 pKa = 9.33TIQTIEE108 pKa = 4.14QNVDD112 pKa = 2.94GYY114 pKa = 11.54SDD116 pKa = 3.84NNGDD120 pKa = 4.17GINDD124 pKa = 3.52QFQVGGPARR133 pKa = 11.84YY134 pKa = 7.58VTRR137 pKa = 11.84AFSDD141 pKa = 3.37GDD143 pKa = 3.96GYY145 pKa = 10.51IVGWIRR151 pKa = 11.84PPYY154 pKa = 10.36SDD156 pKa = 3.58TSSEE160 pKa = 4.17KK161 pKa = 10.12QSQSQAPSGFRR172 pKa = 11.84KK173 pKa = 10.24LKK175 pKa = 10.89DD176 pKa = 3.35EE177 pKa = 4.36VGTFEE182 pKa = 5.09VMVPALNVRR191 pKa = 11.84RR192 pKa = 11.84EE193 pKa = 3.77PSLNGEE199 pKa = 3.97IVACYY204 pKa = 9.54QYY206 pKa = 11.74GMTGTYY212 pKa = 10.62DD213 pKa = 3.12SVYY216 pKa = 11.08VGDD219 pKa = 4.08GYY221 pKa = 10.78IWVSYY226 pKa = 10.44VGASGMRR233 pKa = 11.84NYY235 pKa = 9.76MAVGDD240 pKa = 4.48ADD242 pKa = 3.22GDD244 pKa = 4.55YY245 pKa = 11.31NVNPYY250 pKa = 10.72CKK252 pKa = 10.02FYY254 pKa = 11.49

Molecular weight: 27.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A097PAU0|A0A097PAU0_BPT12 Uncharacterized protein OS=Streptococcus pyogenes phage T12 OX=35344 GN=T12_18 PE=4 SV=1
MM1 pKa = 7.73SEE3 pKa = 4.06KK4 pKa = 10.4RR5 pKa = 11.84WDD7 pKa = 3.36RR8 pKa = 11.84VANKK12 pKa = 10.23NLTEE16 pKa = 4.05MFNRR20 pKa = 11.84AARR23 pKa = 11.84PPGTPIGKK31 pKa = 7.18NTKK34 pKa = 7.24RR35 pKa = 11.84HH36 pKa = 5.65KK37 pKa = 10.69SGEE40 pKa = 4.02LLRR43 pKa = 11.84SRR45 pKa = 11.84RR46 pKa = 11.84LKK48 pKa = 10.52KK49 pKa = 10.55VNSSKK54 pKa = 11.17DD55 pKa = 3.54VITGNFGYY63 pKa = 10.24IKK65 pKa = 10.19DD66 pKa = 4.04YY67 pKa = 10.93APHH70 pKa = 6.57VEE72 pKa = 4.05YY73 pKa = 10.65GHH75 pKa = 6.96RR76 pKa = 11.84IVRR79 pKa = 11.84NGKK82 pKa = 8.3QVGYY86 pKa = 11.31ANGTKK91 pKa = 10.56YY92 pKa = 10.86LFNNVKK98 pKa = 9.38KK99 pKa = 10.39QRR101 pKa = 11.84EE102 pKa = 4.3IYY104 pKa = 9.23RR105 pKa = 11.84QDD107 pKa = 3.14MLNEE111 pKa = 3.91LRR113 pKa = 11.84RR114 pKa = 3.78

Molecular weight: 13.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

11715

36

1146

180.2

20.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.453 ± 0.445

0.504 ± 0.081

6.419 ± 0.232

6.897 ± 0.387

3.799 ± 0.207

6.112 ± 0.383

1.349 ± 0.131

7.119 ± 0.221

8.459 ± 0.351

8.297 ± 0.432

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.185

6.061 ± 0.197

2.8 ± 0.171

4.157 ± 0.205

4.294 ± 0.277

6.47 ± 0.464

6.539 ± 0.365

6.146 ± 0.267

1.417 ± 0.104

4.311 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski