Roseovarius tolerans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseovarius

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3684 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L6CX27|A0A0L6CX27_9RHOB GXGXG motif protein OS=Roseovarius tolerans OX=74031 GN=ROTO_12610 PE=4 SV=1
MM1 pKa = 7.59LKK3 pKa = 7.81TTTALATVTAIFAAPAFAGNVTEE26 pKa = 4.61PAPEE30 pKa = 3.9PVIEE34 pKa = 4.75APAPVPAAPLSPNWTGFYY52 pKa = 10.76GGGQLGWANVDD63 pKa = 3.14ADD65 pKa = 4.75GFDD68 pKa = 5.52DD69 pKa = 5.68DD70 pKa = 5.79DD71 pKa = 4.96SLIGGLTGGYY81 pKa = 10.36DD82 pKa = 3.51YY83 pKa = 11.35DD84 pKa = 3.92FQNGFVVGAGLDD96 pKa = 3.82YY97 pKa = 11.48DD98 pKa = 5.31FLDD101 pKa = 4.14ADD103 pKa = 3.77IGNVATAEE111 pKa = 4.07EE112 pKa = 4.49VFRR115 pKa = 11.84AKK117 pKa = 10.65LRR119 pKa = 11.84GGYY122 pKa = 10.07KK123 pKa = 9.52IGRR126 pKa = 11.84GLAYY130 pKa = 9.48ATGGYY135 pKa = 9.97AWADD139 pKa = 3.46TDD141 pKa = 5.24NLGDD145 pKa = 3.98DD146 pKa = 3.98DD147 pKa = 5.83GYY149 pKa = 11.5FVGAGYY155 pKa = 10.5EE156 pKa = 4.16HH157 pKa = 7.12MVTEE161 pKa = 4.42QVSLGGEE168 pKa = 4.09VLYY171 pKa = 11.13HH172 pKa = 6.13EE173 pKa = 5.63FEE175 pKa = 4.95DD176 pKa = 5.02FNGSGSDD183 pKa = 3.72VEE185 pKa = 4.4ATTVQARR192 pKa = 11.84ATFRR196 pKa = 11.84FF197 pKa = 3.77

Molecular weight: 20.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L6CY74|A0A0L6CY74_9RHOB DUF1330 domain-containing protein OS=Roseovarius tolerans OX=74031 GN=ROTO_09320 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3684

0

3684

1107601

29

2135

300.7

32.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.446 ± 0.057

0.934 ± 0.012

6.074 ± 0.039

5.971 ± 0.043

3.566 ± 0.028

8.817 ± 0.041

2.178 ± 0.019

5.034 ± 0.027

2.889 ± 0.031

10.166 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.867 ± 0.021

2.401 ± 0.023

5.154 ± 0.029

3.16 ± 0.019

7.14 ± 0.044

4.855 ± 0.023

5.469 ± 0.032

7.226 ± 0.039

1.446 ± 0.017

2.208 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski