Lake Sarah-associated circular molecule 7

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126G9G3|A0A126G9G3_9VIRU Replication associated protein OS=Lake Sarah-associated circular molecule 7 OX=1685732 PE=4 SV=1
MM1 pKa = 7.43PASSKK6 pKa = 10.24SWCFTDD12 pKa = 4.9NEE14 pKa = 4.43CLEE17 pKa = 4.44DD18 pKa = 3.4LWNIMEE24 pKa = 4.39STYY27 pKa = 11.02LCYY30 pKa = 10.54GRR32 pKa = 11.84EE33 pKa = 4.08TAPTTGQKK41 pKa = 9.97HH42 pKa = 4.56LQGVIVFATQKK53 pKa = 10.54RR54 pKa = 11.84LAAVKK59 pKa = 9.94KK60 pKa = 8.68LHH62 pKa = 6.14PTCHH66 pKa = 6.31WEE68 pKa = 3.96AAHH71 pKa = 6.33SVPASITYY79 pKa = 9.52CKK81 pKa = 10.49KK82 pKa = 9.73EE83 pKa = 3.81GDD85 pKa = 3.36WFEE88 pKa = 5.19FGTPPAQGKK97 pKa = 10.3RR98 pKa = 11.84NDD100 pKa = 3.93LEE102 pKa = 4.32SVYY105 pKa = 11.44ALIKK109 pKa = 9.88TGAKK113 pKa = 9.22FAEE116 pKa = 4.51VLDD119 pKa = 3.95NHH121 pKa = 6.52PVATIHH127 pKa = 5.12YY128 pKa = 6.12TQGIKK133 pKa = 9.74QAIFAQIKK141 pKa = 9.34HH142 pKa = 6.25RR143 pKa = 11.84PEE145 pKa = 3.52QTPTVNWYY153 pKa = 10.3FGATGAGKK161 pKa = 7.82SHH163 pKa = 7.08RR164 pKa = 11.84AHH166 pKa = 6.85AEE168 pKa = 3.54AVNPYY173 pKa = 9.63IFPNTGRR180 pKa = 11.84FWEE183 pKa = 5.57GYY185 pKa = 8.93EE186 pKa = 4.21GQTHH190 pKa = 6.98VIFDD194 pKa = 4.4DD195 pKa = 4.09FRR197 pKa = 11.84PDD199 pKa = 3.21QVPFAKK205 pKa = 10.58LLRR208 pKa = 11.84ILDD211 pKa = 4.03KK212 pKa = 11.19YY213 pKa = 10.48NCTVEE218 pKa = 4.49VKK220 pKa = 10.41GASVPLAATHH230 pKa = 6.38FWITAPEE237 pKa = 4.51SPQEE241 pKa = 4.09MYY243 pKa = 9.81TATNHH248 pKa = 4.94TTGSTWEE255 pKa = 4.38KK256 pKa = 11.09EE257 pKa = 4.2NIQQLVRR264 pKa = 11.84RR265 pKa = 11.84CTVIEE270 pKa = 4.11RR271 pKa = 11.84IIRR274 pKa = 11.84DD275 pKa = 3.37KK276 pKa = 11.11HH277 pKa = 4.48ITNLIIEE284 pKa = 5.0DD285 pKa = 3.72PAGCPEE291 pKa = 4.7RR292 pKa = 11.84GVTDD296 pKa = 3.88IPPLKK301 pKa = 10.1SQKK304 pKa = 10.61KK305 pKa = 9.0LDD307 pKa = 3.86YY308 pKa = 10.45PITDD312 pKa = 3.18HH313 pKa = 6.79MFLFDD318 pKa = 5.55SSDD321 pKa = 3.44EE322 pKa = 4.32FNLCC326 pKa = 4.24

Molecular weight: 36.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126G9G3|A0A126G9G3_9VIRU Replication associated protein OS=Lake Sarah-associated circular molecule 7 OX=1685732 PE=4 SV=1
MM1 pKa = 7.43PASSKK6 pKa = 10.24SWCFTDD12 pKa = 4.9NEE14 pKa = 4.43CLEE17 pKa = 4.44DD18 pKa = 3.4LWNIMEE24 pKa = 4.39STYY27 pKa = 11.02LCYY30 pKa = 10.54GRR32 pKa = 11.84EE33 pKa = 4.08TAPTTGQKK41 pKa = 9.97HH42 pKa = 4.56LQGVIVFATQKK53 pKa = 10.54RR54 pKa = 11.84LAAVKK59 pKa = 9.94KK60 pKa = 8.68LHH62 pKa = 6.14PTCHH66 pKa = 6.31WEE68 pKa = 3.96AAHH71 pKa = 6.33SVPASITYY79 pKa = 9.52CKK81 pKa = 10.49KK82 pKa = 9.73EE83 pKa = 3.81GDD85 pKa = 3.36WFEE88 pKa = 5.19FGTPPAQGKK97 pKa = 10.3RR98 pKa = 11.84NDD100 pKa = 3.93LEE102 pKa = 4.32SVYY105 pKa = 11.44ALIKK109 pKa = 9.88TGAKK113 pKa = 9.22FAEE116 pKa = 4.51VLDD119 pKa = 3.95NHH121 pKa = 6.52PVATIHH127 pKa = 5.12YY128 pKa = 6.12TQGIKK133 pKa = 9.74QAIFAQIKK141 pKa = 9.34HH142 pKa = 6.25RR143 pKa = 11.84PEE145 pKa = 3.52QTPTVNWYY153 pKa = 10.3FGATGAGKK161 pKa = 7.82SHH163 pKa = 7.08RR164 pKa = 11.84AHH166 pKa = 6.85AEE168 pKa = 3.54AVNPYY173 pKa = 9.63IFPNTGRR180 pKa = 11.84FWEE183 pKa = 5.57GYY185 pKa = 8.93EE186 pKa = 4.21GQTHH190 pKa = 6.98VIFDD194 pKa = 4.4DD195 pKa = 4.09FRR197 pKa = 11.84PDD199 pKa = 3.21QVPFAKK205 pKa = 10.58LLRR208 pKa = 11.84ILDD211 pKa = 4.03KK212 pKa = 11.19YY213 pKa = 10.48NCTVEE218 pKa = 4.49VKK220 pKa = 10.41GASVPLAATHH230 pKa = 6.38FWITAPEE237 pKa = 4.51SPQEE241 pKa = 4.09MYY243 pKa = 9.81TATNHH248 pKa = 4.94TTGSTWEE255 pKa = 4.38KK256 pKa = 11.09EE257 pKa = 4.2NIQQLVRR264 pKa = 11.84RR265 pKa = 11.84CTVIEE270 pKa = 4.11RR271 pKa = 11.84IIRR274 pKa = 11.84DD275 pKa = 3.37KK276 pKa = 11.11HH277 pKa = 4.48ITNLIIEE284 pKa = 5.0DD285 pKa = 3.72PAGCPEE291 pKa = 4.7RR292 pKa = 11.84GVTDD296 pKa = 3.88IPPLKK301 pKa = 10.1SQKK304 pKa = 10.61KK305 pKa = 9.0LDD307 pKa = 3.86YY308 pKa = 10.45PITDD312 pKa = 3.18HH313 pKa = 6.79MFLFDD318 pKa = 5.55SSDD321 pKa = 3.44EE322 pKa = 4.32FNLCC326 pKa = 4.24

Molecular weight: 36.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

326

326

326

326.0

36.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.282 ± 0.0

2.761 ± 0.0

4.908 ± 0.0

6.748 ± 0.0

4.908 ± 0.0

5.521 ± 0.0

4.294 ± 0.0

6.442 ± 0.0

6.442 ± 0.0

5.828 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.227 ± 0.0

3.681 ± 0.0

6.442 ± 0.0

4.294 ± 0.0

3.988 ± 0.0

4.294 ± 0.0

8.896 ± 0.0

5.215 ± 0.0

2.454 ± 0.0

3.374 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski