Mycobacterium phage Florinda

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 117 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1LR35|A0A0K1LR35_9CAUD Minor tail protein OS=Mycobacterium phage Florinda OX=1675549 GN=FLORINDA_17 PE=4 SV=1
MM1 pKa = 7.4AQTIHH6 pKa = 6.12VLPVNDD12 pKa = 5.17LIEE15 pKa = 6.26HH16 pKa = 6.88EE17 pKa = 5.15DD18 pKa = 4.37DD19 pKa = 5.63SDD21 pKa = 3.8EE22 pKa = 4.68CPCGPDD28 pKa = 3.17IEE30 pKa = 4.88PVFDD34 pKa = 5.14ADD36 pKa = 4.79GACGWVITHH45 pKa = 6.86HH46 pKa = 6.49SLDD49 pKa = 3.22GRR51 pKa = 11.84EE52 pKa = 3.86RR53 pKa = 11.84FEE55 pKa = 3.97

Molecular weight: 6.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1LRG7|A0A0K1LRG7_9CAUD Uncharacterized protein OS=Mycobacterium phage Florinda OX=1675549 GN=FLORINDA_64 PE=4 SV=1
MM1 pKa = 7.46ARR3 pKa = 11.84LGRR6 pKa = 11.84AWLGAAWRR14 pKa = 11.84GRR16 pKa = 11.84AWQARR21 pKa = 11.84LGMAWLGMARR31 pKa = 11.84PGSAWQAWQGTARR44 pKa = 11.84RR45 pKa = 11.84GAAGRR50 pKa = 11.84GTAWQARR57 pKa = 11.84QGLARR62 pKa = 11.84RR63 pKa = 11.84GMAGMAGLGWATSQTQGG80 pKa = 3.41

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

117

0

117

19064

28

1174

162.9

17.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.622 ± 0.424

1.269 ± 0.166

6.803 ± 0.215

5.775 ± 0.281

2.89 ± 0.182

9.096 ± 0.553

2.198 ± 0.191

4.265 ± 0.165

3.326 ± 0.206

7.181 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.334 ± 0.134

3.399 ± 0.154

6.053 ± 0.18

3.446 ± 0.166

6.961 ± 0.412

5.781 ± 0.285

6.646 ± 0.242

7.118 ± 0.269

2.366 ± 0.155

2.471 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski