bacterium JGI 053

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1208 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A285Z077|A0A285Z077_9BACT Glutamate--cysteine ligase OS=bacterium JGI 053 OX=1855372 GN=SAMN05216486_1082 PE=4 SV=1
MM1 pKa = 7.16PQVHH5 pKa = 4.9VTGVRR10 pKa = 11.84YY11 pKa = 8.7EE12 pKa = 3.89VSRR15 pKa = 11.84EE16 pKa = 4.03SVASALQHH24 pKa = 4.28YY25 pKa = 8.9TGMGLIEE32 pKa = 4.43ARR34 pKa = 11.84NAAEE38 pKa = 4.05EE39 pKa = 4.27AVSGRR44 pKa = 11.84PVSLYY49 pKa = 10.16IEE51 pKa = 5.07DD52 pKa = 3.96FADD55 pKa = 3.73VYY57 pKa = 11.14EE58 pKa = 4.68LADD61 pKa = 3.39ILTGLGVDD69 pKa = 4.28AEE71 pKa = 4.35ADD73 pKa = 3.51EE74 pKa = 5.03SDD76 pKa = 3.48EE77 pKa = 4.17LQYY80 pKa = 11.81

Molecular weight: 8.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A285Z3A8|A0A285Z3A8_9BACT Elongation factor P OS=bacterium JGI 053 OX=1855372 GN=efp PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 8.67PTYY5 pKa = 9.97RR6 pKa = 11.84PRR8 pKa = 11.84NRR10 pKa = 11.84KK11 pKa = 9.49RR12 pKa = 11.84INKK15 pKa = 8.87HH16 pKa = 3.65GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.36GGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.47GRR39 pKa = 11.84HH40 pKa = 3.86QLTVKK45 pKa = 10.41IAGKK49 pKa = 10.25

Molecular weight: 5.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1208

0

1208

381834

29

4097

316.1

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.454 ± 0.107

0.795 ± 0.03

5.509 ± 0.049

6.164 ± 0.086

3.347 ± 0.043

9.198 ± 0.068

2.065 ± 0.036

3.58 ± 0.05

1.984 ± 0.049

9.949 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.959 ± 0.028

2.038 ± 0.046

5.924 ± 0.063

2.817 ± 0.049

8.856 ± 0.072

4.901 ± 0.058

5.26 ± 0.069

8.415 ± 0.073

1.515 ± 0.032

2.271 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski