Flavobacterium sp. GSN2

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; unclassified Flavobacterium

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5511 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A432BUD7|A0A432BUD7_9FLAO Uncharacterized protein (Fragment) OS=Flavobacterium sp. GSN2 OX=2497480 GN=EKL32_28475 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.53KK3 pKa = 10.51FILSVAVLTMIILTTNSCQNDD24 pKa = 3.78DD25 pKa = 4.39GGSSQALTKK34 pKa = 10.16TSPLTNLLLRR44 pKa = 11.84VTHH47 pKa = 6.4NPTLAGKK54 pKa = 9.66SEE56 pKa = 3.99NEE58 pKa = 3.91TNEE61 pKa = 3.58FCFTVNLPVTIEE73 pKa = 4.04NNGQSVTVTSQDD85 pKa = 3.98DD86 pKa = 3.95YY87 pKa = 11.66ATVQNALHH95 pKa = 5.94QCDD98 pKa = 4.52DD99 pKa = 4.21DD100 pKa = 4.08STSVSFVFPINITFADD116 pKa = 4.35GTTQTINSTEE126 pKa = 4.14GLQQVLDD133 pKa = 3.82TCSDD137 pKa = 3.46EE138 pKa = 6.71DD139 pKa = 4.07EE140 pKa = 5.72DD141 pKa = 5.42EE142 pKa = 6.16DD143 pKa = 6.63DD144 pKa = 4.63IEE146 pKa = 4.9CLNIHH151 pKa = 5.82YY152 pKa = 7.72PINITYY158 pKa = 9.78TDD160 pKa = 4.03ANNLSTVVILNTDD173 pKa = 3.43DD174 pKa = 3.22EE175 pKa = 5.56AYY177 pKa = 10.63AFLTNLEE184 pKa = 4.18ATNSIVISYY193 pKa = 9.33PLTITDD199 pKa = 3.52ATGTTVIVNNNDD211 pKa = 3.65DD212 pKa = 4.0LQTAIEE218 pKa = 4.29EE219 pKa = 4.28ADD221 pKa = 4.03SEE223 pKa = 4.71CGHH226 pKa = 7.17HH227 pKa = 7.62DD228 pKa = 3.98GDD230 pKa = 5.81HH231 pKa = 7.28NDD233 pKa = 3.95DD234 pKa = 5.85DD235 pKa = 4.81EE236 pKa = 6.39NNDD239 pKa = 3.47NGGGKK244 pKa = 8.8MSHH247 pKa = 6.66HH248 pKa = 6.95

Molecular weight: 26.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S0ML45|A0A3S0ML45_9FLAO Nucleotidyl transferase AbiEii/AbiGii toxin family protein OS=Flavobacterium sp. GSN2 OX=2497480 GN=EKL32_25935 PE=4 SV=1
MM1 pKa = 7.74PLLLRR6 pKa = 11.84YY7 pKa = 7.42MPPAFTIIPPRR18 pKa = 11.84FTIIAPRR25 pKa = 11.84IAIMPPRR32 pKa = 11.84FTIIAPRR39 pKa = 11.84FTIMAPRR46 pKa = 11.84FTIIAPRR53 pKa = 11.84FTVIAPRR60 pKa = 11.84FTIMLPLFAIITPRR74 pKa = 11.84FTIMLPRR81 pKa = 11.84FAVIAPQLRR90 pKa = 11.84DD91 pKa = 3.25MRR93 pKa = 11.84PRR95 pKa = 11.84FVV97 pKa = 3.24

Molecular weight: 11.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5511

0

5511

1879230

23

3763

341.0

38.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.637 ± 0.034

0.766 ± 0.011

5.277 ± 0.023

6.245 ± 0.036

5.329 ± 0.027

6.378 ± 0.034

1.713 ± 0.015

8.263 ± 0.034

7.855 ± 0.04

9.128 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.31 ± 0.019

6.261 ± 0.038

3.4 ± 0.019

3.444 ± 0.018

3.198 ± 0.021

6.514 ± 0.031

6.092 ± 0.049

6.239 ± 0.026

0.983 ± 0.014

3.967 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski