Escherichia phage Sortsne

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Sortsnevirus; Escherichia virus Sortsne

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6DZC4|A0A4D6DZC4_9CAUD Coat protein OS=Escherichia phage Sortsne OX=2562456 PE=4 SV=1
MM1 pKa = 7.01KK2 pKa = 9.74TLAEE6 pKa = 4.11TLAEE10 pKa = 4.05IQAEE14 pKa = 4.64IARR17 pKa = 11.84QMASGNPSPLVAGMMIHH34 pKa = 7.24AALADD39 pKa = 4.14RR40 pKa = 11.84DD41 pKa = 3.85GLAGVTIDD49 pKa = 5.43LEE51 pKa = 4.23PTEE54 pKa = 4.41WDD56 pKa = 3.76LVDD59 pKa = 6.44DD60 pKa = 4.58EE61 pKa = 4.74AQLRR65 pKa = 11.84ALPGYY70 pKa = 10.69YY71 pKa = 10.15DD72 pKa = 3.61GFF74 pKa = 4.68

Molecular weight: 7.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6DZ41|A0A4D6DZ41_9CAUD Uncharacterized protein OS=Escherichia phage Sortsne OX=2562456 PE=4 SV=1
MM1 pKa = 6.57NTATIRR7 pKa = 11.84TNKK10 pKa = 10.11NGEE13 pKa = 4.03TLADD17 pKa = 3.74LRR19 pKa = 11.84AKK21 pKa = 10.43YY22 pKa = 9.61WGDD25 pKa = 3.19QMGTIFSRR33 pKa = 11.84PNRR36 pKa = 11.84AKK38 pKa = 10.04VVSWLMLAQAQTLAFNAQCLQNRR61 pKa = 11.84LRR63 pKa = 11.84MWAAGPEE70 pKa = 4.11AVKK73 pKa = 9.4ATKK76 pKa = 10.34RR77 pKa = 11.84EE78 pKa = 3.86IRR80 pKa = 11.84EE81 pKa = 4.17TMAHH85 pKa = 6.55ANDD88 pKa = 4.51ALRR91 pKa = 11.84KK92 pKa = 9.89AYY94 pKa = 9.94GAA96 pKa = 4.06

Molecular weight: 10.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12919

44

787

208.4

23.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.682 ± 0.506

1.115 ± 0.174

6.456 ± 0.211

6.626 ± 0.503

2.988 ± 0.145

7.787 ± 0.427

1.943 ± 0.205

5.008 ± 0.246

5.504 ± 0.334

7.826 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.833 ± 0.172

4.041 ± 0.297

4.985 ± 0.195

4.768 ± 0.434

6.161 ± 0.252

5.039 ± 0.313

5.426 ± 0.249

6.285 ± 0.283

1.61 ± 0.143

2.918 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski