Jeotgalibacillus proteolyticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Jeotgalibacillus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4022 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2S5GFZ8|A0A2S5GFZ8_9BACL Cold-shock protein OS=Jeotgalibacillus proteolyticus OX=2082395 GN=C4B60_00230 PE=4 SV=1
MM1 pKa = 7.46NISKK5 pKa = 10.7GLLLTAAGTAILLAGCNTGGDD26 pKa = 3.67GAEE29 pKa = 4.19RR30 pKa = 11.84DD31 pKa = 3.6SSEE34 pKa = 4.8PIVISGKK41 pKa = 9.44PWTEE45 pKa = 3.72QYY47 pKa = 10.75ILPHH51 pKa = 6.74ILQQYY56 pKa = 8.95IEE58 pKa = 4.38HH59 pKa = 6.72HH60 pKa = 6.4SDD62 pKa = 3.35YY63 pKa = 11.17DD64 pKa = 3.72VEE66 pKa = 4.2LQEE69 pKa = 4.77GLGEE73 pKa = 4.19VAILQPAMEE82 pKa = 4.77NGEE85 pKa = 3.57IDD87 pKa = 4.8LYY89 pKa = 11.47VEE91 pKa = 4.08YY92 pKa = 10.21TGTGLEE98 pKa = 4.0AVLDD102 pKa = 4.02EE103 pKa = 4.38RR104 pKa = 11.84AEE106 pKa = 4.13EE107 pKa = 4.81GEE109 pKa = 4.22SSEE112 pKa = 5.15SILDD116 pKa = 3.61RR117 pKa = 11.84VRR119 pKa = 11.84QGYY122 pKa = 8.07EE123 pKa = 3.55EE124 pKa = 4.36EE125 pKa = 5.59FNITWLEE132 pKa = 3.82PLGFANDD139 pKa = 3.33YY140 pKa = 10.42TMAFSEE146 pKa = 4.75DD147 pKa = 3.47ADD149 pKa = 4.34FQASTYY155 pKa = 10.42SDD157 pKa = 3.7LVEE160 pKa = 4.62LSSEE164 pKa = 4.21LTFGAPHH171 pKa = 7.21AFYY174 pKa = 10.61EE175 pKa = 4.38RR176 pKa = 11.84QGDD179 pKa = 4.26GYY181 pKa = 10.99DD182 pKa = 4.39AIVEE186 pKa = 4.27AYY188 pKa = 10.12GFDD191 pKa = 3.81FAQTEE196 pKa = 4.34SLDD199 pKa = 3.78ANIMYY204 pKa = 10.19QAVQQGQVDD213 pKa = 4.77VIPAFTTDD221 pKa = 2.89GRR223 pKa = 11.84IEE225 pKa = 4.22RR226 pKa = 11.84FNLQTTEE233 pKa = 4.31DD234 pKa = 3.99DD235 pKa = 4.28LGFFPKK241 pKa = 10.23YY242 pKa = 9.96DD243 pKa = 3.53AAPVVRR249 pKa = 11.84QDD251 pKa = 3.34VLEE254 pKa = 4.79AYY256 pKa = 9.44PEE258 pKa = 4.26LEE260 pKa = 3.91EE261 pKa = 4.59VLNGLAGQISEE272 pKa = 4.83EE273 pKa = 4.7DD274 pKa = 3.65MQEE277 pKa = 3.67MNAKK281 pKa = 9.81VDD283 pKa = 3.49IDD285 pKa = 4.21GEE287 pKa = 4.3DD288 pKa = 3.58YY289 pKa = 11.34AAVAEE294 pKa = 4.71EE295 pKa = 4.11FLQSKK300 pKa = 10.12GLLDD304 pKa = 3.59

Molecular weight:
33.63 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2S5G692|A0A2S5G692_9BACL Sporulation initiation inhibitor Soj OS=Jeotgalibacillus proteolyticus OX=2082395 GN=C4B60_20800 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.59RR3 pKa = 11.84TFQPNKK9 pKa = 8.24RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84SKK14 pKa = 9.83VHH16 pKa = 5.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.36VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.8VLSAA44 pKa = 4.11

Molecular weight:
5.28 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4022

0

4022

1171169

27

1920

291.2

32.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.402 ± 0.04

0.638 ± 0.011

5.134 ± 0.03

7.834 ± 0.051

4.569 ± 0.038

7.06 ± 0.035

2.089 ± 0.021

7.528 ± 0.041

6.391 ± 0.037

9.89 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.772 ± 0.017

4.14 ± 0.026

3.689 ± 0.021

3.685 ± 0.026

4.161 ± 0.027

6.347 ± 0.024

5.354 ± 0.023

6.866 ± 0.029

1.101 ± 0.016

3.348 ± 0.022

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski