Amino acid dipepetide frequency for Nocardia asteroides NBRC 15531

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.989AlaAla: 22.989 ± 0.178	0.975AlaCys: 0.975 ± 0.025	9.406AlaAsp: 9.406 ± 0.095	8.995AlaGlu: 8.995 ± 0.086	3.489AlaPhe: 3.489 ± 0.042	13.185AlaGly: 13.185 ± 0.094	2.738AlaHis: 2.738 ± 0.037	5.037AlaIle: 5.037 ± 0.055	2.688AlaLys: 2.688 ± 0.046	14.279AlaLeu: 14.279 ± 0.107	2.766AlaMet: 2.766 ± 0.045	2.235AlaAsn: 2.235 ± 0.036	7.163AlaPro: 7.163 ± 0.084	4.141AlaGln: 4.141 ± 0.053	9.609AlaArg: 9.609 ± 0.076	5.283AlaSer: 5.283 ± 0.055	8.35AlaThr: 8.35 ± 0.07	12.828AlaVal: 12.828 ± 0.108	1.697AlaTrp: 1.697 ± 0.029	2.403AlaTyr: 2.403 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	1.124CysAla: 1.124 ± 0.028	0.086CysCys: 0.086 ± 0.008	0.421CysAsp: 0.421 ± 0.014	0.329CysGlu: 0.329 ± 0.013	0.201CysPhe: 0.201 ± 0.011	0.868CysGly: 0.868 ± 0.021	0.17CysHis: 0.17 ± 0.009	0.182CysIle: 0.182 ± 0.01	0.101CysLys: 0.101 ± 0.007	0.641CysLeu: 0.641 ± 0.018	0.099CysMet: 0.099 ± 0.007	0.115CysAsn: 0.115 ± 0.007	0.438CysPro: 0.438 ± 0.016	0.156CysGln: 0.156 ± 0.007	0.524CysArg: 0.524 ± 0.019	0.407CysSer: 0.407 ± 0.013	0.464CysThr: 0.464 ± 0.016	0.616CysVal: 0.616 ± 0.017	0.101CysTrp: 0.101 ± 0.007	0.155CysTyr: 0.155 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.956AspAla: 7.956 ± 0.081	0.396AspCys: 0.396 ± 0.015	3.938AspAsp: 3.938 ± 0.058	3.642AspGlu: 3.642 ± 0.044	1.785AspPhe: 1.785 ± 0.031	5.828AspGly: 5.828 ± 0.063	1.413AspHis: 1.413 ± 0.025	2.402AspIle: 2.402 ± 0.035	1.179AspLys: 1.179 ± 0.028	6.616AspLeu: 6.616 ± 0.075	0.867AspMet: 0.867 ± 0.022	1.125AspAsn: 1.125 ± 0.026	4.902AspPro: 4.902 ± 0.068	1.824AspGln: 1.824 ± 0.029	5.231AspArg: 5.231 ± 0.061	2.844AspSer: 2.844 ± 0.037	3.589AspThr: 3.589 ± 0.039	4.476AspVal: 4.476 ± 0.051	0.942AspTrp: 0.942 ± 0.022	1.407AspTyr: 1.407 ± 0.027	0.0AspXaa: 0.0 ± 0.0
Glu	5.862GluAla: 5.862 ± 0.058	0.31GluCys: 0.31 ± 0.012	2.148GluAsp: 2.148 ± 0.037	2.163GluGlu: 2.163 ± 0.037	1.972GluPhe: 1.972 ± 0.035	3.122GluGly: 3.122 ± 0.038	1.585GluHis: 1.585 ± 0.026	2.69GluIle: 2.69 ± 0.037	1.076GluLys: 1.076 ± 0.029	7.163GluLeu: 7.163 ± 0.09	0.908GluMet: 0.908 ± 0.024	1.005GluAsn: 1.005 ± 0.025	3.334GluPro: 3.334 ± 0.045	2.355GluGln: 2.355 ± 0.037	4.678GluArg: 4.678 ± 0.05	2.77GluSer: 2.77 ± 0.04	2.793GluThr: 2.793 ± 0.037	4.462GluVal: 4.462 ± 0.058	0.723GluTrp: 0.723 ± 0.018	1.1GluTyr: 1.1 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	4.463PheAla: 4.463 ± 0.053	0.243PheCys: 0.243 ± 0.012	2.466PheAsp: 2.466 ± 0.046	1.468PheGlu: 1.468 ± 0.033	0.896PhePhe: 0.896 ± 0.021	3.528PheGly: 3.528 ± 0.05	0.607PheHis: 0.607 ± 0.015	0.768PheIle: 0.768 ± 0.019	0.4PheLys: 0.4 ± 0.015	2.629PheLeu: 2.629 ± 0.043	0.382PheMet: 0.382 ± 0.014	0.512PheAsn: 0.512 ± 0.016	1.402PhePro: 1.402 ± 0.026	0.706PheGln: 0.706 ± 0.022	1.796PheArg: 1.796 ± 0.029	1.373PheSer: 1.373 ± 0.029	2.191PheThr: 2.191 ± 0.035	2.6PheVal: 2.6 ± 0.037	0.424PheTrp: 0.424 ± 0.014	0.616PheTyr: 0.616 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	11.328GlyAla: 11.328 ± 0.1	0.763GlyCys: 0.763 ± 0.023	5.044GlyAsp: 5.044 ± 0.062	4.612GlyGlu: 4.612 ± 0.061	3.09GlyPhe: 3.09 ± 0.042	7.835GlyGly: 7.835 ± 0.116	1.978GlyHis: 1.978 ± 0.035	4.123GlyIle: 4.123 ± 0.056	2.197GlyLys: 2.197 ± 0.037	8.974GlyLeu: 8.974 ± 0.083	2.058GlyMet: 2.058 ± 0.029	1.797GlyAsn: 1.797 ± 0.043	4.726GlyPro: 4.726 ± 0.049	2.566GlyGln: 2.566 ± 0.048	6.439GlyArg: 6.439 ± 0.054	5.119GlySer: 5.119 ± 0.065	6.025GlyThr: 6.025 ± 0.06	7.591GlyVal: 7.591 ± 0.059	1.585GlyTrp: 1.585 ± 0.032	2.352GlyTyr: 2.352 ± 0.039	0.0GlyXaa: 0.0 ± 0.0
His	2.577HisAla: 2.577 ± 0.036	0.198HisCys: 0.198 ± 0.01	1.284HisAsp: 1.284 ± 0.026	1.044HisGlu: 1.044 ± 0.023	0.638HisPhe: 0.638 ± 0.017	2.076HisGly: 2.076 ± 0.032	0.66HisHis: 0.66 ± 0.021	0.708HisIle: 0.708 ± 0.019	0.283HisLys: 0.283 ± 0.012	2.232HisLeu: 2.232 ± 0.032	0.323HisMet: 0.323 ± 0.013	0.418HisAsn: 0.418 ± 0.014	1.71HisPro: 1.71 ± 0.028	0.571HisGln: 0.571 ± 0.018	2.011HisArg: 2.011 ± 0.031	1.038HisSer: 1.038 ± 0.026	1.298HisThr: 1.298 ± 0.025	1.507HisVal: 1.507 ± 0.027	0.353HisTrp: 0.353 ± 0.012	0.5HisTyr: 0.5 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	6.455IleAla: 6.455 ± 0.055	0.296IleCys: 0.296 ± 0.012	3.213IleAsp: 3.213 ± 0.046	2.517IleGlu: 2.517 ± 0.038	0.891IlePhe: 0.891 ± 0.023	4.489IleGly: 4.489 ± 0.052	0.679IleHis: 0.679 ± 0.019	1.153IleIle: 1.153 ± 0.031	0.661IleLys: 0.661 ± 0.017	3.009IleLeu: 3.009 ± 0.041	0.536IleMet: 0.536 ± 0.018	0.788IleAsn: 0.788 ± 0.02	2.192IlePro: 2.192 ± 0.032	0.813IleGln: 0.813 ± 0.02	2.469IleArg: 2.469 ± 0.034	1.974IleSer: 1.974 ± 0.038	2.716IleThr: 2.716 ± 0.038	3.735IleVal: 3.735 ± 0.052	0.389IleTrp: 0.389 ± 0.013	0.698IleTyr: 0.698 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	2.317LysAla: 2.317 ± 0.042	0.089LysCys: 0.089 ± 0.007	0.89LysAsp: 0.89 ± 0.024	0.798LysGlu: 0.798 ± 0.025	0.575LysPhe: 0.575 ± 0.016	1.294LysGly: 1.294 ± 0.025	0.394LysHis: 0.394 ± 0.014	0.904LysIle: 0.904 ± 0.022	0.553LysLys: 0.553 ± 0.021	1.967LysLeu: 1.967 ± 0.035	0.427LysMet: 0.427 ± 0.015	0.442LysAsn: 0.442 ± 0.016	1.234LysPro: 1.234 ± 0.029	0.677LysGln: 0.677 ± 0.02	1.22LysArg: 1.22 ± 0.027	1.062LysSer: 1.062 ± 0.028	1.162LysThr: 1.162 ± 0.026	1.663LysVal: 1.663 ± 0.035	0.253LysTrp: 0.253 ± 0.01	0.446LysTyr: 0.446 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	15.709LeuAla: 15.709 ± 0.116	0.793LeuCys: 0.793 ± 0.02	7.283LeuAsp: 7.283 ± 0.08	4.182LeuGlu: 4.182 ± 0.048	2.815LeuPhe: 2.815 ± 0.044	9.33LeuGly: 9.33 ± 0.072	2.076LeuHis: 2.076 ± 0.033	4.056LeuIle: 4.056 ± 0.048	1.308LeuLys: 1.308 ± 0.03	10.572LeuLeu: 10.572 ± 0.11	1.487LeuMet: 1.487 ± 0.028	1.684LeuAsn: 1.684 ± 0.029	6.289LeuPro: 6.289 ± 0.068	2.05LeuGln: 2.05 ± 0.037	8.565LeuArg: 8.565 ± 0.065	5.488LeuSer: 5.488 ± 0.053	7.04LeuThr: 7.04 ± 0.064	8.955LeuVal: 8.955 ± 0.074	1.213LeuTrp: 1.213 ± 0.026	1.666LeuTyr: 1.666 ± 0.034	0.0LeuXaa: 0.0 ± 0.0
Met	2.192MetAla: 2.192 ± 0.038	0.137MetCys: 0.137 ± 0.008	0.835MetAsp: 0.835 ± 0.021	0.612MetGlu: 0.612 ± 0.018	0.594MetPhe: 0.594 ± 0.019	1.25MetGly: 1.25 ± 0.029	0.365MetHis: 0.365 ± 0.012	0.884MetIle: 0.884 ± 0.022	0.352MetLys: 0.352 ± 0.013	1.914MetLeu: 1.914 ± 0.028	0.332MetMet: 0.332 ± 0.014	0.482MetAsn: 0.482 ± 0.016	1.131MetPro: 1.131 ± 0.027	0.503MetGln: 0.503 ± 0.015	1.401MetArg: 1.401 ± 0.028	1.425MetSer: 1.425 ± 0.031	1.639MetThr: 1.639 ± 0.031	1.509MetVal: 1.509 ± 0.03	0.223MetTrp: 0.223 ± 0.011	0.326MetTyr: 0.326 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.334AsnAla: 2.334 ± 0.036	0.147AsnCys: 0.147 ± 0.009	0.927AsnAsp: 0.927 ± 0.023	0.81AsnGlu: 0.81 ± 0.023	0.532AsnPhe: 0.532 ± 0.015	1.804AsnGly: 1.804 ± 0.038	0.392AsnHis: 0.392 ± 0.015	0.795AsnIle: 0.795 ± 0.018	0.406AsnLys: 0.406 ± 0.014	1.8AsnLeu: 1.8 ± 0.036	0.33AsnMet: 0.33 ± 0.016	0.465AsnAsn: 0.465 ± 0.019	1.539AsnPro: 1.539 ± 0.029	0.562AsnGln: 0.562 ± 0.018	1.361AsnArg: 1.361 ± 0.027	1.015AsnSer: 1.015 ± 0.021	1.162AsnThr: 1.162 ± 0.026	1.387AsnVal: 1.387 ± 0.026	0.303AsnTrp: 0.303 ± 0.014	0.467AsnTyr: 0.467 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	8.917ProAla: 8.917 ± 0.095	0.277ProCys: 0.277 ± 0.013	4.531ProAsp: 4.531 ± 0.051	3.921ProGlu: 3.921 ± 0.047	1.54ProPhe: 1.54 ± 0.026	6.124ProGly: 6.124 ± 0.07	1.173ProHis: 1.173 ± 0.027	2.145ProIle: 2.145 ± 0.031	1.099ProLys: 1.099 ± 0.023	5.105ProLeu: 5.105 ± 0.068	1.068ProMet: 1.068 ± 0.024	1.094ProAsn: 1.094 ± 0.028	3.197ProPro: 3.197 ± 0.068	1.718ProGln: 1.718 ± 0.036	3.679ProArg: 3.679 ± 0.052	2.92ProSer: 2.92 ± 0.049	3.636ProThr: 3.636 ± 0.047	5.553ProVal: 5.553 ± 0.062	0.798ProTrp: 0.798 ± 0.022	1.081ProTyr: 1.081 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.621GlnAla: 3.621 ± 0.045	0.163GlnCys: 0.163 ± 0.01	1.142GlnAsp: 1.142 ± 0.025	1.052GlnGlu: 1.052 ± 0.024	0.863GlnPhe: 0.863 ± 0.02	1.953GlnGly: 1.953 ± 0.038	0.625GlnHis: 0.625 ± 0.018	1.323GlnIle: 1.323 ± 0.028	0.487GlnLys: 0.487 ± 0.016	3.486GlnLeu: 3.486 ± 0.047	0.526GlnMet: 0.526 ± 0.017	0.518GlnAsn: 0.518 ± 0.019	1.809GlnPro: 1.809 ± 0.042	1.256GlnGln: 1.256 ± 0.041	2.673GlnArg: 2.673 ± 0.045	1.272GlnSer: 1.272 ± 0.029	1.397GlnThr: 1.397 ± 0.028	2.68GlnVal: 2.68 ± 0.043	0.5GlnTrp: 0.5 ± 0.015	0.601GlnTyr: 0.601 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	9.688ArgAla: 9.688 ± 0.089	0.521ArgCys: 0.521 ± 0.017	4.27ArgAsp: 4.27 ± 0.056	3.972ArgGlu: 3.972 ± 0.052	2.487ArgPhe: 2.487 ± 0.035	5.399ArgGly: 5.399 ± 0.054	1.824ArgHis: 1.824 ± 0.03	3.62ArgIle: 3.62 ± 0.041	1.57ArgLys: 1.57 ± 0.03	7.677ArgLeu: 7.677 ± 0.075	1.837ArgMet: 1.837 ± 0.03	1.435ArgAsn: 1.435 ± 0.026	4.332ArgPro: 4.332 ± 0.054	2.069ArgGln: 2.069 ± 0.042	6.73ArgArg: 6.73 ± 0.086	3.837ArgSer: 3.837 ± 0.045	5.105ArgThr: 5.105 ± 0.061	5.935ArgVal: 5.935 ± 0.059	1.41ArgTrp: 1.41 ± 0.025	1.834ArgTyr: 1.834 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	7.092SerAla: 7.092 ± 0.063	0.384SerCys: 0.384 ± 0.014	2.677SerAsp: 2.677 ± 0.037	2.284SerGlu: 2.284 ± 0.037	1.508SerPhe: 1.508 ± 0.025	5.527SerGly: 5.527 ± 0.062	0.902SerHis: 0.902 ± 0.022	1.938SerIle: 1.938 ± 0.035	0.977SerLys: 0.977 ± 0.025	4.562SerLeu: 4.562 ± 0.051	1.209SerMet: 1.209 ± 0.022	0.9SerAsn: 0.9 ± 0.022	2.905SerPro: 2.905 ± 0.045	1.216SerGln: 1.216 ± 0.026	3.365SerArg: 3.365 ± 0.043	2.709SerSer: 2.709 ± 0.046	3.484SerThr: 3.484 ± 0.046	4.232SerVal: 4.232 ± 0.044	0.903SerTrp: 0.903 ± 0.023	1.098SerTyr: 1.098 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	9.29ThrAla: 9.29 ± 0.071	0.398ThrCys: 0.398 ± 0.013	4.028ThrAsp: 4.028 ± 0.048	3.746ThrGlu: 3.746 ± 0.048	1.727ThrPhe: 1.727 ± 0.031	6.801ThrGly: 6.801 ± 0.076	1.204ThrHis: 1.204 ± 0.022	2.332ThrIle: 2.332 ± 0.034	1.135ThrLys: 1.135 ± 0.029	5.953ThrLeu: 5.953 ± 0.058	1.111ThrMet: 1.111 ± 0.026	1.047ThrAsn: 1.047 ± 0.022	4.165ThrPro: 4.165 ± 0.058	1.445ThrGln: 1.445 ± 0.026	3.954ThrArg: 3.954 ± 0.047	3.049ThrSer: 3.049 ± 0.04	4.427ThrThr: 4.427 ± 0.062	6.585ThrVal: 6.585 ± 0.056	0.858ThrTrp: 0.858 ± 0.02	1.227ThrTyr: 1.227 ± 0.026	0.0ThrXaa: 0.0 ± 0.0
Val	12.305ValAla: 12.305 ± 0.1	0.692ValCys: 0.692 ± 0.017	5.837ValAsp: 5.837 ± 0.056	4.448ValGlu: 4.448 ± 0.047	2.674ValPhe: 2.674 ± 0.038	6.888ValGly: 6.888 ± 0.065	1.855ValHis: 1.855 ± 0.03	3.418ValIle: 3.418 ± 0.041	1.314ValLys: 1.314 ± 0.027	9.877ValLeu: 9.877 ± 0.078	1.275ValMet: 1.275 ± 0.025	1.711ValAsn: 1.711 ± 0.03	5.113ValPro: 5.113 ± 0.06	2.06ValGln: 2.06 ± 0.03	6.677ValArg: 6.677 ± 0.061	4.342ValSer: 4.342 ± 0.045	5.843ValThr: 5.843 ± 0.057	8.644ValVal: 8.644 ± 0.075	1.016ValTrp: 1.016 ± 0.024	1.541ValTyr: 1.541 ± 0.025	0.0ValXaa: 0.0 ± 0.0
Trp	1.591TrpAla: 1.591 ± 0.027	0.139TrpCys: 0.139 ± 0.009	0.745TrpAsp: 0.745 ± 0.02	0.624TrpGlu: 0.624 ± 0.017	0.509TrpPhe: 0.509 ± 0.017	0.924TrpGly: 0.924 ± 0.023	0.358TrpHis: 0.358 ± 0.013	0.617TrpIle: 0.617 ± 0.02	0.287TrpLys: 0.287 ± 0.012	1.668TrpLeu: 1.668 ± 0.038	0.311TrpMet: 0.311 ± 0.012	0.384TrpAsn: 0.384 ± 0.014	0.765TrpPro: 0.765 ± 0.019	0.625TrpGln: 0.625 ± 0.018	1.26TrpArg: 1.26 ± 0.024	0.951TrpSer: 0.951 ± 0.021	0.969TrpThr: 0.969 ± 0.023	1.024TrpVal: 1.024 ± 0.022	0.347TrpTrp: 0.347 ± 0.017	0.297TrpTyr: 0.297 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.447TyrAla: 2.447 ± 0.042	0.19TyrCys: 0.19 ± 0.011	1.275TyrAsp: 1.275 ± 0.024	1.001TyrGlu: 1.001 ± 0.023	0.685TyrPhe: 0.685 ± 0.019	1.974TyrGly: 1.974 ± 0.038	0.43TyrHis: 0.43 ± 0.016	0.576TyrIle: 0.576 ± 0.02	0.297TyrLys: 0.297 ± 0.012	2.438TyrLeu: 2.438 ± 0.036	0.253TyrMet: 0.253 ± 0.011	0.382TyrAsn: 0.382 ± 0.012	1.23TyrPro: 1.23 ± 0.021	0.635TyrGln: 0.635 ± 0.018	1.906TyrArg: 1.906 ± 0.031	0.995TyrSer: 0.995 ± 0.023	1.195TyrThr: 1.195 ± 0.025	1.55TyrVal: 1.55 ± 0.032	0.356TyrTrp: 0.356 ± 0.015	0.449TyrTyr: 0.449 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6459 proteins (2104526 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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