Pseudoxanthomonas sp. CF125

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Pseudoxanthomonas; unclassified Pseudoxanthomonas

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3691 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1BTW0|A0A1H1BTW0_9GAMM ATP-binding protein Uup OS=Pseudoxanthomonas sp. CF125 OX=1855303 GN=uup PE=3 SV=1
MM1 pKa = 7.35NKK3 pKa = 9.03VFSLVWSRR11 pKa = 11.84SKK13 pKa = 10.61QALVVASEE21 pKa = 4.17LASAQGRR28 pKa = 11.84KK29 pKa = 8.61EE30 pKa = 3.69GRR32 pKa = 11.84PRR34 pKa = 11.84LRR36 pKa = 11.84DD37 pKa = 3.41ATLGAVLLGCGIAMALPAAHH57 pKa = 6.42AQEE60 pKa = 4.22LTIEE64 pKa = 4.29RR65 pKa = 11.84DD66 pKa = 3.17GDD68 pKa = 3.66VCTARR73 pKa = 11.84VEE75 pKa = 4.42GQAEE79 pKa = 4.34GRR81 pKa = 11.84QIDD84 pKa = 4.16CALFDD89 pKa = 3.74QANVGINAVVGSGYY103 pKa = 10.59FLADD107 pKa = 3.49GAGDD111 pKa = 3.95GSDD114 pKa = 4.25DD115 pKa = 3.8PTVSGDD121 pKa = 3.24GALAAGRR128 pKa = 11.84NSSALNNATTAVGAEE143 pKa = 4.16SAAVGLGASAFGQGAMAFGDD163 pKa = 3.56ASVAIGQSAYY173 pKa = 10.68ASGDD177 pKa = 3.67GPVMGLGNTAIGASASAEE195 pKa = 4.0AEE197 pKa = 4.02QGGTAVGALSLAAGTGSTAIGGNAQAYY224 pKa = 10.15GDD226 pKa = 3.97GSLVGGFGSGANGEE240 pKa = 4.29AGVALGANSRR250 pKa = 11.84ALGGDD255 pKa = 3.58FGVAIGGSSYY265 pKa = 11.22AIGQGATSVGGLAWAEE281 pKa = 4.18GNYY284 pKa = 10.56SLASGYY290 pKa = 11.02YY291 pKa = 7.79STAVGDD297 pKa = 3.67GSIAFGGGLGVDD309 pKa = 4.18LDD311 pKa = 4.31GDD313 pKa = 4.16GLIGPGEE320 pKa = 3.98ATIAAGYY327 pKa = 9.07HH328 pKa = 5.39AVALGYY334 pKa = 9.7QAAALGDD341 pKa = 3.75GTLALGDD348 pKa = 3.66QAQALQLNATAVGTMSVAAGEE369 pKa = 4.49SSTATGANSVATGAEE384 pKa = 4.07SSAYY388 pKa = 9.55GANSYY393 pKa = 11.58ADD395 pKa = 3.91ADD397 pKa = 3.86YY398 pKa = 9.88STAIGSGSLALGSGSTAIGAGANALGTEE426 pKa = 4.79SVASGYY432 pKa = 11.23GSVASGEE439 pKa = 3.97YY440 pKa = 10.4SIAQGSSSQALGEE453 pKa = 3.85ASIAIGVQSVASGTDD468 pKa = 3.31SIGIGAGSQAGDD480 pKa = 3.31FGTAVGTLSNATGYY494 pKa = 9.98GASAFGDD501 pKa = 3.85NAWATNDD508 pKa = 3.1FSLAMGANSTSLGIGGIAIGTDD530 pKa = 3.39SYY532 pKa = 11.89VGADD536 pKa = 3.58ASHH539 pKa = 7.02GIAIGNLVMANGKK552 pKa = 9.82NSIAMGGLWTTAGADD567 pKa = 3.44GAIAIGGTKK576 pKa = 10.14ACTSFMCIEE585 pKa = 4.37EE586 pKa = 4.2NTLAWGINSVALGAGATTQADD607 pKa = 3.99GATALGSGAQAGGIDD622 pKa = 4.32SISIGMDD629 pKa = 2.87SRR631 pKa = 11.84AGSVNSVGIGARR643 pKa = 11.84TYY645 pKa = 11.3ALGDD649 pKa = 3.34NSVALGADD657 pKa = 3.45SVTSRR662 pKa = 11.84ANTVTVGSEE671 pKa = 3.7WTGMTRR677 pKa = 11.84QITGVADD684 pKa = 3.46GTEE687 pKa = 4.2ANDD690 pKa = 4.3AVNLSQLNAVADD702 pKa = 4.22SVGDD706 pKa = 3.83ANDD709 pKa = 3.59YY710 pKa = 11.25LSVNGLGDD718 pKa = 3.59GTDD721 pKa = 3.11AAIAEE726 pKa = 4.57GEE728 pKa = 4.18NASAAGANAVAIGIEE743 pKa = 4.55SSALGYY749 pKa = 10.14QSNAVGDD756 pKa = 3.88YY757 pKa = 8.52STAIGGSSLALGAGSSALGTGASAIGDD784 pKa = 3.53YY785 pKa = 11.06SSANGYY791 pKa = 8.94GAIAEE796 pKa = 4.5GSNSNAFGANAAAIGNSSFAGGDD819 pKa = 3.43SAQANGGGSVALGASSIANGSNGVSVGAGAEE850 pKa = 4.36SNGFFAAAIGGYY862 pKa = 10.29ALADD866 pKa = 3.69SPFSVAIGGFATASGWQATAIGSGAFASGFNATATGAGAMAGDD909 pKa = 4.0GGVAIGAGAASGYY922 pKa = 8.87WFYY925 pKa = 11.65DD926 pKa = 3.02EE927 pKa = 4.43TTGEE931 pKa = 4.01NTFIGSSGTAVGQNAAATGMFSTALGGFSDD961 pKa = 5.13GSFASAGATGMYY973 pKa = 8.52STALGAGAYY982 pKa = 9.63AGSLDD987 pKa = 3.55GVVPGDD993 pKa = 3.42YY994 pKa = 7.56TTAVGAEE1001 pKa = 3.98SWAVGDD1007 pKa = 4.21SATSLGFNSYY1017 pKa = 10.09AQSANSTVVGANAWTTVDD1035 pKa = 3.73ADD1037 pKa = 3.52NSVALGEE1044 pKa = 4.44GSLADD1049 pKa = 3.81RR1050 pKa = 11.84ANTVSVGAAQDD1061 pKa = 3.22WTDD1064 pKa = 2.96AGGNVHH1070 pKa = 7.03AAIDD1074 pKa = 3.75RR1075 pKa = 11.84QITNVAAGTEE1085 pKa = 4.1ATDD1088 pKa = 4.2AVNLAQLNEE1097 pKa = 4.13VADD1100 pKa = 3.87NLGEE1104 pKa = 4.11FTQNAVAYY1112 pKa = 10.22DD1113 pKa = 4.18DD1114 pKa = 5.58DD1115 pKa = 4.56SHH1117 pKa = 7.72DD1118 pKa = 3.94KK1119 pKa = 10.13LTLEE1123 pKa = 4.52GAGGSTISNLAAGTQASDD1141 pKa = 3.25AVNVEE1146 pKa = 3.96QLNSVAAAFGGGASLGANGMLVAPTYY1172 pKa = 8.94TIQGGGYY1179 pKa = 10.1ANAGDD1184 pKa = 4.05AFAAVDD1190 pKa = 3.88GMLSGLNGRR1199 pKa = 11.84LTQIEE1204 pKa = 4.37QNLGNGGGIGGPPTGTGDD1222 pKa = 3.47GLAIGTGSNATDD1234 pKa = 3.53TTDD1237 pKa = 3.18TAVGNGANVGADD1249 pKa = 2.83NGTAVGNNSSIAASATDD1266 pKa = 3.74SVAIGADD1273 pKa = 3.25ASVTAASGTAIGQGATVTGAGSVALGQGSVADD1305 pKa = 3.71QANTVSVGSAGNEE1318 pKa = 3.28RR1319 pKa = 11.84RR1320 pKa = 11.84VTNVAAGVAATDD1332 pKa = 3.75AANVGQMQAGDD1343 pKa = 3.77AATLASANDD1352 pKa = 3.76YY1353 pKa = 10.61TDD1355 pKa = 3.19ATATEE1360 pKa = 4.33TLSAANAYY1368 pKa = 8.05TDD1370 pKa = 4.19SRR1372 pKa = 11.84LQTFDD1377 pKa = 4.96DD1378 pKa = 4.84DD1379 pKa = 4.27FTALRR1384 pKa = 11.84NDD1386 pKa = 2.91VDD1388 pKa = 4.22HH1389 pKa = 7.37RR1390 pKa = 11.84FSQQDD1395 pKa = 3.02RR1396 pKa = 11.84RR1397 pKa = 11.84IDD1399 pKa = 3.22KK1400 pKa = 9.68MGAMSSAMMNMSINAAGSRR1419 pKa = 11.84SPRR1422 pKa = 11.84GRR1424 pKa = 11.84IAVGAGWQNGEE1435 pKa = 4.1SALSVGYY1442 pKa = 9.95SKK1444 pKa = 11.0QIGTRR1449 pKa = 11.84ASFSIGGAFSSDD1461 pKa = 3.06EE1462 pKa = 3.96KK1463 pKa = 10.96SAGVGFGVDD1472 pKa = 3.19LL1473 pKa = 4.58

Molecular weight: 139.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H1GIX3|A0A1H1GIX3_9GAMM CubicO group peptidase beta-lactamase class C family OS=Pseudoxanthomonas sp. CF125 OX=1855303 GN=SAMN05216569_3235 PE=4 SV=1
MM1 pKa = 7.74SDD3 pKa = 3.9LVALTALEE11 pKa = 4.34PTTEE15 pKa = 4.37RR16 pKa = 11.84YY17 pKa = 8.9LAACWSDD24 pKa = 3.15STRR27 pKa = 11.84RR28 pKa = 11.84AYY30 pKa = 10.33AGDD33 pKa = 3.56LRR35 pKa = 11.84DD36 pKa = 3.59FLKK39 pKa = 10.52WGGCLPASEE48 pKa = 4.32QQVVEE53 pKa = 4.49YY54 pKa = 10.16INEE57 pKa = 3.89RR58 pKa = 11.84AKK60 pKa = 10.61VFRR63 pKa = 11.84VRR65 pKa = 11.84TLEE68 pKa = 3.77RR69 pKa = 11.84RR70 pKa = 11.84LTGIGMAHH78 pKa = 7.76ALQGHH83 pKa = 5.73TDD85 pKa = 3.62PTKK88 pKa = 9.72STLIKK93 pKa = 10.64KK94 pKa = 10.07LMRR97 pKa = 11.84GVKK100 pKa = 9.06RR101 pKa = 11.84VHH103 pKa = 6.13GVTPRR108 pKa = 11.84QAQPILHH115 pKa = 7.31ADD117 pKa = 4.14LQQMFEE123 pKa = 4.21RR124 pKa = 11.84TVGLTQTRR132 pKa = 11.84DD133 pKa = 3.07RR134 pKa = 11.84ALLMLGFSCAFRR146 pKa = 11.84RR147 pKa = 11.84SEE149 pKa = 4.18VVSLNVEE156 pKa = 4.03DD157 pKa = 5.82LSFTEE162 pKa = 4.4AGLTVRR168 pKa = 11.84LRR170 pKa = 11.84RR171 pKa = 11.84SKK173 pKa = 10.88NDD175 pKa = 3.01QYY177 pKa = 11.92GKK179 pKa = 8.47TRR181 pKa = 11.84LIGVPHH187 pKa = 6.2GTGDD191 pKa = 3.4ACTVLAVRR199 pKa = 11.84AWLYY203 pKa = 11.24ASGIRR208 pKa = 11.84SGPLFRR214 pKa = 11.84RR215 pKa = 11.84LLKK218 pKa = 10.4SKK220 pKa = 10.63RR221 pKa = 11.84SGARR225 pKa = 11.84LSDD228 pKa = 3.33QSVSLIIKK236 pKa = 9.44RR237 pKa = 11.84YY238 pKa = 8.44AVAVGLPVDD247 pKa = 5.09RR248 pKa = 11.84ISGHH252 pKa = 5.18SLRR255 pKa = 11.84AGFVTSAVRR264 pKa = 11.84AGASLVSIQRR274 pKa = 11.84QTGHH278 pKa = 7.11ASLDD282 pKa = 3.19MLARR286 pKa = 11.84YY287 pKa = 9.07IRR289 pKa = 11.84EE290 pKa = 4.1LDD292 pKa = 3.61PFILNAHH299 pKa = 5.93DD300 pKa = 3.71HH301 pKa = 5.92LRR303 pKa = 4.37

Molecular weight: 33.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3691

0

3691

1245779

31

4227

337.5

36.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.413 ± 0.057

0.805 ± 0.014

5.712 ± 0.026

5.474 ± 0.036

3.547 ± 0.025

8.599 ± 0.041

2.088 ± 0.019

4.369 ± 0.025

3.256 ± 0.035

10.498 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.191 ± 0.02

2.818 ± 0.036

5.221 ± 0.03

3.744 ± 0.027

7.023 ± 0.045

5.728 ± 0.036

5.158 ± 0.053

7.248 ± 0.033

1.565 ± 0.02

2.543 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski