Synechococcus phage S-RIP2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Sednavirus; Synechococcus virus SRIP2

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4NV57|M4NV57_9CAUD Uncharacterized protein OS=Synechococcus phage S-RIP2 OX=754040 GN=SWQG_00050 PE=4 SV=1
MM1 pKa = 7.1TTFCHH6 pKa = 7.12DD7 pKa = 3.23MHH9 pKa = 7.51DD10 pKa = 4.06YY11 pKa = 11.38LCMRR15 pKa = 11.84FDD17 pKa = 5.87DD18 pKa = 4.18PTEE21 pKa = 4.15LKK23 pKa = 10.79DD24 pKa = 3.49VANYY28 pKa = 9.26GCSGGVSGFIYY39 pKa = 9.29YY40 pKa = 9.05TEE42 pKa = 3.93INEE45 pKa = 4.05FFDD48 pKa = 4.45AYY50 pKa = 10.32EE51 pKa = 3.97EE52 pKa = 4.1QIEE55 pKa = 4.75NIMDD59 pKa = 3.69MAGITYY65 pKa = 10.21QDD67 pKa = 4.08LVPNADD73 pKa = 3.89SIQALKK79 pKa = 10.82CSAVWFAVEE88 pKa = 5.77DD89 pKa = 4.05YY90 pKa = 11.11CQMALNVLEE99 pKa = 4.7NEE101 pKa = 4.3AALAAA106 pKa = 4.52

Molecular weight: 11.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4NV33|M4NV33_9CAUD Uncharacterized protein OS=Synechococcus phage S-RIP2 OX=754040 GN=SWQG_00022 PE=4 SV=1
MM1 pKa = 7.79SDD3 pKa = 3.39VEE5 pKa = 4.25LMARR9 pKa = 11.84LRR11 pKa = 11.84EE12 pKa = 4.01RR13 pKa = 11.84EE14 pKa = 3.91EE15 pKa = 3.87RR16 pKa = 11.84NRR18 pKa = 11.84NAKK21 pKa = 9.81LKK23 pKa = 9.97YY24 pKa = 10.03RR25 pKa = 11.84GVAYY29 pKa = 10.56NKK31 pKa = 10.03NDD33 pKa = 3.21QCLEE37 pKa = 4.23NINLQTQRR45 pKa = 11.84QRR47 pKa = 11.84PFLTIRR53 pKa = 11.84LRR55 pKa = 11.84VNKK58 pKa = 9.41CSSVADD64 pKa = 3.64TVVIKK69 pKa = 10.79SLSVVNKK76 pKa = 10.04RR77 pKa = 11.84SAA79 pKa = 2.85

Molecular weight: 9.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13085

49

1558

242.3

26.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.987 ± 0.356

0.833 ± 0.167

6.206 ± 0.384

6.068 ± 0.414

3.615 ± 0.192

7.489 ± 0.636

1.467 ± 0.156

5.082 ± 0.196

5.006 ± 0.437

7.765 ± 0.388

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.728 ± 0.306

5.151 ± 0.367

4.54 ± 0.291

4.494 ± 0.353

4.807 ± 0.354

6.779 ± 0.454

7.841 ± 0.794

6.519 ± 0.253

1.2 ± 0.142

3.424 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski