Enterococcus phage vB_EfaM_Ef2.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Brockvirinae; Kochikohdavirus; unclassified Kochikohdavirus

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 204 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6DTR5|A0A4D6DTR5_9CAUD Uncharacterized protein OS=Enterococcus phage vB_EfaM_Ef2.1 OX=2546633 PE=4 SV=1
MM1 pKa = 7.46NNLTAQDD8 pKa = 4.42LLDD11 pKa = 4.31EE12 pKa = 4.56LLEE15 pKa = 5.11LKK17 pKa = 10.69DD18 pKa = 3.41QGHH21 pKa = 6.64ALEE24 pKa = 5.22DD25 pKa = 4.16YY26 pKa = 10.39IVVGEE31 pKa = 4.54PINPYY36 pKa = 10.41DD37 pKa = 4.44HH38 pKa = 6.88YY39 pKa = 11.72LEE41 pKa = 4.32FDD43 pKa = 3.34GWHH46 pKa = 6.79LDD48 pKa = 3.29SNTNMIILKK57 pKa = 10.09GG58 pKa = 3.56

Molecular weight: 6.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6DV12|A0A4D6DV12_9CAUD Putative glycerophosphoryl diester phosphodiesterase OS=Enterococcus phage vB_EfaM_Ef2.1 OX=2546633 PE=4 SV=1
MM1 pKa = 7.32ARR3 pKa = 11.84KK4 pKa = 8.59GQRR7 pKa = 11.84PVLFTDD13 pKa = 3.92SKK15 pKa = 11.52AILGNLTRR23 pKa = 11.84AVVDD27 pKa = 4.04EE28 pKa = 4.49VLSDD32 pKa = 3.76AQDD35 pKa = 3.1VALRR39 pKa = 11.84NGSSVQRR46 pKa = 11.84MPSYY50 pKa = 11.07LIVTEE55 pKa = 4.18SRR57 pKa = 11.84MAKK60 pKa = 10.3NGVIDD65 pKa = 4.76LKK67 pKa = 11.18PFFARR72 pKa = 11.84SNKK75 pKa = 9.5KK76 pKa = 9.97KK77 pKa = 10.28YY78 pKa = 9.02NKK80 pKa = 9.77KK81 pKa = 10.5GEE83 pKa = 4.0WYY85 pKa = 9.87LYY87 pKa = 10.29IPISMKK93 pKa = 8.85TRR95 pKa = 11.84NMSRR99 pKa = 11.84RR100 pKa = 11.84LYY102 pKa = 10.69DD103 pKa = 3.32EE104 pKa = 4.78LRR106 pKa = 11.84AVPVGTKK113 pKa = 9.89PVTVKK118 pKa = 9.86MDD120 pKa = 3.45YY121 pKa = 10.83LYY123 pKa = 10.88DD124 pKa = 3.64RR125 pKa = 11.84RR126 pKa = 11.84KK127 pKa = 9.8QSPSVSSINYY137 pKa = 8.74KK138 pKa = 10.25PKK140 pKa = 8.93STNVTVIPQSWGKK153 pKa = 7.84GTRR156 pKa = 11.84NTYY159 pKa = 9.47VAFRR163 pKa = 11.84TVNANSPANSWIINRR178 pKa = 11.84RR179 pKa = 11.84NVNDD183 pKa = 4.21DD184 pKa = 4.03DD185 pKa = 3.93MSKK188 pKa = 9.59TMLRR192 pKa = 11.84NIDD195 pKa = 4.92RR196 pKa = 11.84LMKK199 pKa = 10.14WKK201 pKa = 10.49LKK203 pKa = 10.69NLGGG207 pKa = 3.72

Molecular weight: 23.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

204

0

204

41249

37

1825

202.2

22.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.503 ± 0.194

0.81 ± 0.088

6.211 ± 0.154

8.58 ± 0.262

3.748 ± 0.098

6.262 ± 0.199

1.522 ± 0.095

6.618 ± 0.172

7.913 ± 0.193

8.543 ± 0.19

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.524 ± 0.075

5.804 ± 0.15

3.009 ± 0.146

3.459 ± 0.179

4.039 ± 0.113

6.25 ± 0.16

6.33 ± 0.19

7.115 ± 0.163

1.086 ± 0.07

4.674 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski