Pantoea stewartii subsp. stewartii DC283

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Pantoea; Pantoea stewartii; Pantoea stewartii subsp. stewartii

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4581 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H3RLU1|H3RLU1_PANSE Uncharacterized protein OS=Pantoea stewartii subsp. stewartii DC283 OX=660596 GN=CKS_5525 PE=4 SV=1
MM1 pKa = 7.54SFQFSDD7 pKa = 3.56EE8 pKa = 4.18QQTLTVYY15 pKa = 10.43HH16 pKa = 6.6YY17 pKa = 10.23DD18 pKa = 3.45ASTGEE23 pKa = 4.24YY24 pKa = 9.98LSSSDD29 pKa = 4.14EE30 pKa = 4.36VIPPHH35 pKa = 6.41TGLPADD41 pKa = 4.28CTEE44 pKa = 4.21TAPPEE49 pKa = 4.32AVTGYY54 pKa = 10.84AWIYY58 pKa = 10.31TSGAWLQVEE67 pKa = 4.47DD68 pKa = 3.93HH69 pKa = 7.05RR70 pKa = 11.84GTTVYY75 pKa = 10.79VKK77 pKa = 10.47ASRR80 pKa = 11.84VGVLVTTLGALLDD93 pKa = 4.27TVTTIAPATEE103 pKa = 3.71YY104 pKa = 10.78DD105 pKa = 3.19AWNATTGAWEE115 pKa = 3.9VDD117 pKa = 3.61TAAKK121 pKa = 9.93LAAEE125 pKa = 4.25SEE127 pKa = 4.43AAVEE131 pKa = 4.19QVNTLMGTANQQVDD145 pKa = 3.78IYY147 pKa = 9.26TDD149 pKa = 3.58AVSLPTATDD158 pKa = 3.25EE159 pKa = 4.14DD160 pKa = 4.67TLMFTAWKK168 pKa = 8.04TYY170 pKa = 9.18RR171 pKa = 11.84VALNKK176 pKa = 9.69VDD178 pKa = 3.6TTDD181 pKa = 3.62PEE183 pKa = 4.46NIVWPTVPTLASVTAAAA200 pKa = 4.8

Molecular weight: 21.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H3RIV6|H3RIV6_PANSE Putative uroporphyrinogen III methylase OS=Pantoea stewartii subsp. stewartii DC283 OX=660596 GN=hemX PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4581

0

4581

1321671

23

2497

288.5

31.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.153 ± 0.042

1.049 ± 0.013

5.326 ± 0.031

5.466 ± 0.038

3.652 ± 0.026

7.289 ± 0.04

2.358 ± 0.02

5.461 ± 0.031

4.101 ± 0.035

10.824 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.725 ± 0.017

3.607 ± 0.027

4.516 ± 0.025

4.787 ± 0.035

6.083 ± 0.033

6.008 ± 0.027

5.419 ± 0.032

6.976 ± 0.031

1.517 ± 0.016

2.68 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski