Rhodovulum bhavnagarense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodovulum

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3085 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R2RH35|A0A4R2RH35_9RHOB Site-specific DNA-methyltransferase (adenine-specific) OS=Rhodovulum bhavnagarense OX=992286 GN=EV663_12416 PE=3 SV=1
MM1 pKa = 7.71TYY3 pKa = 10.7DD4 pKa = 4.9CDD6 pKa = 5.05DD7 pKa = 3.65STPTEE12 pKa = 4.2TANATADD19 pKa = 3.48TSSFAGNVIGLWEE32 pKa = 4.34FDD34 pKa = 4.52DD35 pKa = 4.57GCSYY39 pKa = 10.56YY40 pKa = 11.12ARR42 pKa = 11.84DD43 pKa = 3.44TGLDD47 pKa = 3.43DD48 pKa = 5.49GVAQNGTYY56 pKa = 10.63EE57 pKa = 4.22NGANDD62 pKa = 3.52YY63 pKa = 11.07GGWLTLDD70 pKa = 4.44GYY72 pKa = 11.07NDD74 pKa = 3.78RR75 pKa = 11.84VDD77 pKa = 3.94VDD79 pKa = 4.36GKK81 pKa = 9.5DD82 pKa = 3.36TPFDD86 pKa = 4.0LSAGTIQTRR95 pKa = 11.84FIADD99 pKa = 3.85GSYY102 pKa = 10.45SKK104 pKa = 11.14CGGTQLEE111 pKa = 4.86GGTLVNRR118 pKa = 11.84GEE120 pKa = 4.04YY121 pKa = 9.96ADD123 pKa = 5.36RR124 pKa = 11.84YY125 pKa = 9.8DD126 pKa = 3.28EE127 pKa = 5.43GYY129 pKa = 10.76FGLSTTASGAVMVEE143 pKa = 4.51HH144 pKa = 6.42YY145 pKa = 10.68AAGASVTLSTQDD157 pKa = 2.56GFYY160 pKa = 11.33AMGDD164 pKa = 3.85EE165 pKa = 4.44VTVTYY170 pKa = 10.55SWDD173 pKa = 3.45ADD175 pKa = 3.46GGATLIVLNATTGQTAMLSDD195 pKa = 3.87DD196 pKa = 3.91TVTGLTLDD204 pKa = 3.76IGDD207 pKa = 4.41NDD209 pKa = 4.54DD210 pKa = 3.73EE211 pKa = 4.84NFTFGARR218 pKa = 11.84EE219 pKa = 3.93VDD221 pKa = 3.26DD222 pKa = 5.13GKK224 pKa = 11.4YY225 pKa = 10.51DD226 pKa = 3.4DD227 pKa = 5.51HH228 pKa = 8.52FDD230 pKa = 3.43GKK232 pKa = 10.17IAFVAVYY239 pKa = 8.41DD240 pKa = 3.91QPVAAGDD247 pKa = 3.9GFVEE251 pKa = 5.0GTAGDD256 pKa = 4.23DD257 pKa = 5.01LIDD260 pKa = 3.66AAYY263 pKa = 9.33TGDD266 pKa = 4.17PEE268 pKa = 5.12EE269 pKa = 5.44DD270 pKa = 3.6MIDD273 pKa = 3.59AGDD276 pKa = 4.73AILPGEE282 pKa = 4.84APDD285 pKa = 5.22DD286 pKa = 4.98DD287 pKa = 4.19IVLAGDD293 pKa = 3.83GNDD296 pKa = 3.19TVLAGEE302 pKa = 4.91GDD304 pKa = 3.94DD305 pKa = 3.87EE306 pKa = 5.2VYY308 pKa = 10.88AGTGIDD314 pKa = 3.66EE315 pKa = 4.62VEE317 pKa = 4.74GGTGDD322 pKa = 3.73DD323 pKa = 4.81LIYY326 pKa = 10.71GGSGADD332 pKa = 3.52ILDD335 pKa = 4.05GQAGNDD341 pKa = 3.88TIFGDD346 pKa = 4.04GGNAPTIATITFEE359 pKa = 4.64SEE361 pKa = 3.52DD362 pKa = 3.53AGYY365 pKa = 10.51LNTMGVYY372 pKa = 10.08TIDD375 pKa = 4.78PDD377 pKa = 3.45TGAITNVEE385 pKa = 3.81IAFEE389 pKa = 4.14NTSAIGSGGNLTQGDD404 pKa = 4.81SYY406 pKa = 11.41TYY408 pKa = 9.23VTGPGAQVGVFLIADD423 pKa = 4.83GYY425 pKa = 11.27DD426 pKa = 3.53LNDD429 pKa = 3.4YY430 pKa = 10.37AALGEE435 pKa = 4.34GSFLFLDD442 pKa = 4.15ASGNPATLDD451 pKa = 3.49TPNPSLVFVAEE462 pKa = 5.18DD463 pKa = 3.68GTQTALSGEE472 pKa = 4.27IFHH475 pKa = 6.51TAAHH479 pKa = 5.77GTNLGLNPDD488 pKa = 4.32GLLHH492 pKa = 5.81TAGYY496 pKa = 9.98SEE498 pKa = 4.85NPDD501 pKa = 3.06GSVTLGFEE509 pKa = 5.15DD510 pKa = 4.2INGLGDD516 pKa = 3.53QDD518 pKa = 3.96YY519 pKa = 10.49DD520 pKa = 4.11DD521 pKa = 4.87PVFTVAIEE529 pKa = 4.22GAGGSLNNAHH539 pKa = 7.14FDD541 pKa = 3.93LEE543 pKa = 4.37NPADD547 pKa = 3.88ILTEE551 pKa = 4.38GDD553 pKa = 3.6DD554 pKa = 3.96SLIGGDD560 pKa = 3.9GADD563 pKa = 3.51VIYY566 pKa = 10.75GGGGNDD572 pKa = 3.86TIHH575 pKa = 6.98AGSGLTSQSPDD586 pKa = 3.01LGYY589 pKa = 10.02PGLFDD594 pKa = 6.27ADD596 pKa = 3.97TDD598 pKa = 4.14PEE600 pKa = 4.39DD601 pKa = 4.72DD602 pKa = 3.73RR603 pKa = 11.84DD604 pKa = 4.08YY605 pKa = 12.0VDD607 pKa = 5.42GGDD610 pKa = 3.93GNDD613 pKa = 5.31LITTGDD619 pKa = 4.0DD620 pKa = 3.21ADD622 pKa = 4.25TIYY625 pKa = 11.19GGTGNDD631 pKa = 3.77TIDD634 pKa = 3.63AGVDD638 pKa = 2.85ADD640 pKa = 4.96EE641 pKa = 4.39IHH643 pKa = 6.92GGAGDD648 pKa = 3.81DD649 pKa = 4.4SIIGGEE655 pKa = 4.24GSDD658 pKa = 4.21TIAGGAGDD666 pKa = 3.73DD667 pKa = 4.17TIYY670 pKa = 11.27GGLDD674 pKa = 3.16PAYY677 pKa = 9.11PDD679 pKa = 4.14SLNIPDD685 pKa = 4.22NTDD688 pKa = 3.39LVPDD692 pKa = 4.07NGRR695 pKa = 11.84DD696 pKa = 3.73LIDD699 pKa = 4.48GGDD702 pKa = 4.07GNDD705 pKa = 4.76LIYY708 pKa = 10.85GQDD711 pKa = 4.11DD712 pKa = 4.32DD713 pKa = 3.99DD714 pKa = 4.68TIYY717 pKa = 11.26GGAGNDD723 pKa = 4.08TIDD726 pKa = 4.47AGIDD730 pKa = 3.32DD731 pKa = 5.46DD732 pKa = 4.86YY733 pKa = 11.98VDD735 pKa = 4.2GGEE738 pKa = 4.28GADD741 pKa = 3.87VIYY744 pKa = 10.85SGAGSDD750 pKa = 3.85TIEE753 pKa = 5.12GGDD756 pKa = 4.12GDD758 pKa = 4.18DD759 pKa = 4.54TIHH762 pKa = 7.2GGGDD766 pKa = 3.44NDD768 pKa = 4.02VLSGGADD775 pKa = 3.18RR776 pKa = 11.84DD777 pKa = 3.19IFYY780 pKa = 10.0IDD782 pKa = 3.56SVGMPGGSTNTTVHH796 pKa = 6.39GGNTGVDD803 pKa = 3.24WDD805 pKa = 4.37TLNLSALISGGWTITHH821 pKa = 6.9LVKK824 pKa = 11.09NPDD827 pKa = 3.64GDD829 pKa = 4.25PDD831 pKa = 3.93GLGWDD836 pKa = 4.14GQITLQDD843 pKa = 3.17GGGQYY848 pKa = 11.66ANINFTNIEE857 pKa = 4.13EE858 pKa = 4.73IIPCFTPGTLIATPKK873 pKa = 10.31GEE875 pKa = 4.09RR876 pKa = 11.84PVEE879 pKa = 3.86EE880 pKa = 4.44LRR882 pKa = 11.84EE883 pKa = 3.95GDD885 pKa = 4.32KK886 pKa = 11.27VITRR890 pKa = 11.84DD891 pKa = 3.49NGIQEE896 pKa = 4.13IRR898 pKa = 11.84WIGTTNVSSEE908 pKa = 3.84RR909 pKa = 11.84LAHH912 pKa = 6.86DD913 pKa = 3.43KK914 pKa = 11.1HH915 pKa = 7.17LMPVLIRR922 pKa = 11.84AGALGHH928 pKa = 6.3GLPEE932 pKa = 5.05RR933 pKa = 11.84DD934 pKa = 3.33MLVSPQHH941 pKa = 6.0RR942 pKa = 11.84VLVANDD948 pKa = 3.21RR949 pKa = 11.84TALYY953 pKa = 10.34FEE955 pKa = 4.27EE956 pKa = 5.98RR957 pKa = 11.84EE958 pKa = 4.11VLVAAKK964 pKa = 10.08HH965 pKa = 5.15LVNNRR970 pKa = 11.84SIQRR974 pKa = 11.84VAPRR978 pKa = 11.84ALSYY982 pKa = 10.79LHH984 pKa = 6.82FMFDD988 pKa = 3.02QHH990 pKa = 6.54EE991 pKa = 4.6VVLSDD996 pKa = 3.99GAWTEE1001 pKa = 4.14SFQPGDD1007 pKa = 3.58YY1008 pKa = 10.27TLGGMGNAQRR1018 pKa = 11.84GEE1020 pKa = 3.95IFEE1023 pKa = 5.23LFPEE1027 pKa = 4.63LQTEE1031 pKa = 4.13EE1032 pKa = 4.27GLRR1035 pKa = 11.84DD1036 pKa = 3.39YY1037 pKa = 11.13VAARR1041 pKa = 11.84RR1042 pKa = 11.84TLRR1045 pKa = 11.84RR1046 pKa = 11.84HH1047 pKa = 5.0EE1048 pKa = 4.08AVLLGSEE1055 pKa = 4.56FF1056 pKa = 3.56

Molecular weight: 110.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R2RCF0|A0A4R2RCF0_9RHOB DNA polymerase IV OS=Rhodovulum bhavnagarense OX=992286 GN=dinB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84AKK41 pKa = 10.7LSAA44 pKa = 4.0

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3085

0

3085

951992

28

2677

308.6

33.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.078 ± 0.07

0.872 ± 0.014

6.035 ± 0.043

5.968 ± 0.046

3.541 ± 0.028

8.866 ± 0.05

2.053 ± 0.021

4.928 ± 0.03

2.792 ± 0.035

10.307 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.718 ± 0.022

2.3 ± 0.021

5.349 ± 0.033

3.008 ± 0.023

7.615 ± 0.046

4.622 ± 0.03

5.219 ± 0.031

7.293 ± 0.038

1.343 ± 0.018

2.094 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski