Haliea sp. SAOS-164

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Halieaceae; Haliea; unclassified Haliea

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4187 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z0M9A0|A0A4Z0M9A0_9GAMM Glycerophosphodiester phosphodiesterase OS=Haliea sp. SAOS-164 OX=2562682 GN=glpQ PE=4 SV=1
MM1 pKa = 7.4SRR3 pKa = 11.84NLRR6 pKa = 11.84LLACTGVVLGLSSVYY21 pKa = 10.71SLPASGALSLSCSLPYY37 pKa = 9.91THH39 pKa = 7.26GKK41 pKa = 8.48PGKK44 pKa = 9.78DD45 pKa = 3.1CTGKK49 pKa = 8.43HH50 pKa = 5.31TIEE53 pKa = 3.91IQVAGGSPQTEE64 pKa = 4.2VQAEE68 pKa = 4.28LLALDD73 pKa = 4.19PNGVILGALPVEE85 pKa = 4.54NHH87 pKa = 6.82DD88 pKa = 4.01PAHH91 pKa = 5.81LRR93 pKa = 11.84SRR95 pKa = 11.84IDD97 pKa = 3.23EE98 pKa = 4.55DD99 pKa = 4.68DD100 pKa = 3.67YY101 pKa = 12.1SLRR104 pKa = 11.84TTNRR108 pKa = 11.84GVHH111 pKa = 5.29VVAAPIPLLSVTAVDD126 pKa = 3.98GDD128 pKa = 4.23EE129 pKa = 4.31TVHH132 pKa = 6.93AYY134 pKa = 10.62CSDD137 pKa = 3.32VPYY140 pKa = 10.97LRR142 pKa = 11.84LYY144 pKa = 10.5QPTGQVATAGDD155 pKa = 3.84TVDD158 pKa = 3.51VVLAATGLEE167 pKa = 4.14PASLQVFVDD176 pKa = 4.4GVEE179 pKa = 3.77IAAALGLTPATDD191 pKa = 4.12FPGGPYY197 pKa = 10.1AGNVSIGGEE206 pKa = 4.01NVEE209 pKa = 4.14ITDD212 pKa = 4.3LVVDD216 pKa = 5.02LGDD219 pKa = 3.6IEE221 pKa = 4.92EE222 pKa = 4.7PSSNTVSFTLEE233 pKa = 3.65GLPAGGHH240 pKa = 5.8VIFADD245 pKa = 4.07GEE247 pKa = 4.23RR248 pKa = 11.84LANRR252 pKa = 11.84PTRR255 pKa = 11.84GPVSDD260 pKa = 4.25ACHH263 pKa = 6.55IDD265 pKa = 3.46DD266 pKa = 5.43MEE268 pKa = 4.9DD269 pKa = 3.0AGQFAVFGVDD279 pKa = 2.72IEE281 pKa = 5.0SPFEE285 pKa = 3.97QQVVNAVPTPVSGVVKK301 pKa = 10.46HH302 pKa = 6.14GLPIAATKK310 pKa = 10.55INGAALDD317 pKa = 3.66VSGQSFVAGDD327 pKa = 3.82GKK329 pKa = 9.69FTADD333 pKa = 2.77QYY335 pKa = 11.53EE336 pKa = 4.06YY337 pKa = 10.66HH338 pKa = 6.9FDD340 pKa = 3.52EE341 pKa = 6.66AIPQTDD347 pKa = 3.41LRR349 pKa = 11.84ADD351 pKa = 4.16LDD353 pKa = 4.08AGSAPLGSFDD363 pKa = 4.43RR364 pKa = 11.84GSNRR368 pKa = 11.84LTVDD372 pKa = 3.57ALDD375 pKa = 3.92TYY377 pKa = 11.06GNRR380 pKa = 11.84AFDD383 pKa = 3.75EE384 pKa = 4.52LFFAVGGVQSPTAPLEE400 pKa = 4.11TLAANALGFNVEE412 pKa = 4.31RR413 pKa = 11.84SVSPLLMVALDD424 pKa = 3.84EE425 pKa = 4.92AEE427 pKa = 4.15EE428 pKa = 4.7AIQNSFVVGLTEE440 pKa = 6.07DD441 pKa = 5.07GIQALMDD448 pKa = 3.9QKK450 pKa = 11.3CPDD453 pKa = 3.36AGQAFKK459 pKa = 11.05EE460 pKa = 4.5SVQSKK465 pKa = 8.99IAEE468 pKa = 3.89IAPISKK474 pKa = 9.93KK475 pKa = 10.36ISGGCSCSPNVEE487 pKa = 3.7IGITGSSIDD496 pKa = 4.31ANAISCPVTFQDD508 pKa = 3.79GKK510 pKa = 10.12IAVSIDD516 pKa = 3.48LPDD519 pKa = 3.37VTVYY523 pKa = 10.61GSAYY527 pKa = 8.94GHH529 pKa = 6.69CKK531 pKa = 9.42DD532 pKa = 4.09TVAGVCVAEE541 pKa = 4.33TTVDD545 pKa = 3.1ITSTTTISDD554 pKa = 3.86LSLSFNITEE563 pKa = 4.35GQFLGDD569 pKa = 3.77PPEE572 pKa = 4.26EE573 pKa = 4.01PVFVTGTSSVSTSGGSDD590 pKa = 3.35VDD592 pKa = 4.93CLAEE596 pKa = 3.94VCNWAIEE603 pKa = 4.56GIVTIFTFGTVDD615 pKa = 4.59LDD617 pKa = 3.49LTPAIDD623 pKa = 3.57VSQVTEE629 pKa = 4.16FNQAIGASEE638 pKa = 4.75PDD640 pKa = 4.87PIDD643 pKa = 4.43LGDD646 pKa = 3.43IKK648 pKa = 10.33IDD650 pKa = 3.57EE651 pKa = 4.54EE652 pKa = 4.22EE653 pKa = 4.28VEE655 pKa = 4.42EE656 pKa = 4.87FGQASLGGALEE667 pKa = 4.34SVEE670 pKa = 3.85ITPQGFLATLSGNFEE685 pKa = 4.27TLAVDD690 pKa = 3.86TDD692 pKa = 3.94VQPTPGAVVDD702 pKa = 4.08VAPAPTMQIPNAGDD716 pKa = 3.44ATVLLSDD723 pKa = 4.01EE724 pKa = 5.08VINQLFSSMAEE735 pKa = 3.87SGGLKK740 pKa = 8.61TACQYY745 pKa = 10.05TDD747 pKa = 3.2KK748 pKa = 11.28SVDD751 pKa = 3.58DD752 pKa = 4.71VLPTDD757 pKa = 4.83CDD759 pKa = 3.94ALDD762 pKa = 3.89GAAASGMCHH771 pKa = 7.58AIRR774 pKa = 11.84GTDD777 pKa = 3.59CEE779 pKa = 4.42TLAGDD784 pKa = 5.36GITGTAIKK792 pKa = 10.46QGVCHH797 pKa = 6.33GASGDD802 pKa = 3.83TCSGIAGSDD811 pKa = 3.67LEE813 pKa = 5.25RR814 pKa = 11.84NTCSGTPPTTLAAYY828 pKa = 9.29NQVLFCSRR836 pKa = 11.84QDD838 pKa = 3.34NPPRR842 pKa = 11.84FLIQDD847 pKa = 3.78DD848 pKa = 4.29AGTPATVEE856 pKa = 3.98TAVRR860 pKa = 11.84LSDD863 pKa = 3.61LSVAIVVDD871 pKa = 3.92RR872 pKa = 11.84DD873 pKa = 3.23NDD875 pKa = 3.69GFAEE879 pKa = 4.55LNDD882 pKa = 3.89TPDD885 pKa = 3.88CFGSDD890 pKa = 2.98ASVSGDD896 pKa = 3.16CALYY900 pKa = 8.39NTCLDD905 pKa = 4.14LNLVTTSSIATEE917 pKa = 4.25NLDD920 pKa = 3.89CPAGVPGFVTDD931 pKa = 3.9VVDD934 pKa = 4.3FQILNRR940 pKa = 11.84QEE942 pKa = 4.6GVVCGAAQEE951 pKa = 4.55TVDD954 pKa = 3.82GQLVGTSNDD963 pKa = 3.8DD964 pKa = 3.54DD965 pKa = 4.1TVDD968 pKa = 5.3LIGEE972 pKa = 4.33NVDD975 pKa = 3.51TYY977 pKa = 11.68APPLCVEE984 pKa = 4.3GLDD987 pKa = 3.59LGGFVTLQNPKK998 pKa = 9.9IIAVEE1003 pKa = 4.02TDD1005 pKa = 2.67GDD1007 pKa = 4.11ADD1009 pKa = 3.64FQEE1012 pKa = 4.97YY1013 pKa = 10.26IGITGEE1019 pKa = 4.03VAAPP1023 pKa = 3.49

Molecular weight: 105.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Z0M0I0|A0A4Z0M0I0_9GAMM Methyltransferase OS=Haliea sp. SAOS-164 OX=2562682 GN=E4634_12215 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.91VLSAA44 pKa = 4.11

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4187

0

4187

1444718

26

3160

345.0

37.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.639 ± 0.056

1.115 ± 0.013

6.034 ± 0.028

6.36 ± 0.034

3.612 ± 0.022

8.242 ± 0.035

2.185 ± 0.018

4.529 ± 0.026

2.616 ± 0.029

10.87 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.018

2.8 ± 0.02

5.032 ± 0.026

3.887 ± 0.02

6.925 ± 0.037

5.607 ± 0.029

4.807 ± 0.027

7.216 ± 0.028

1.433 ± 0.014

2.789 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski