Pseudomonas phage phi12

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Vidaverviricetes; Mindivirales; Cystoviridae; Cystovirus; Pseudomonas virus phi12

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q94M08|Q94M08_9VIRU p15 OS=Pseudomonas phage phi12 OX=161736 GN=15 PE=4 SV=1
MM1 pKa = 8.09DD2 pKa = 6.7FITDD6 pKa = 3.29MSKK9 pKa = 9.82NQRR12 pKa = 11.84LEE14 pKa = 3.88LQNRR18 pKa = 11.84LAQYY22 pKa = 7.41EE23 pKa = 4.25TSLMVMSHH31 pKa = 6.24NGDD34 pKa = 3.45VPVITGFNVMRR45 pKa = 11.84VTTMLDD51 pKa = 3.08ALKK54 pKa = 11.0VEE56 pKa = 4.67LPAVAVLGDD65 pKa = 3.82DD66 pKa = 4.26AQDD69 pKa = 3.35LAYY72 pKa = 11.02VFGARR77 pKa = 11.84PLAVGVNIIRR87 pKa = 11.84VVDD90 pKa = 3.75VPGQQPSALVDD101 pKa = 3.51AEE103 pKa = 4.48LGALHH108 pKa = 6.45EE109 pKa = 4.39VSMVRR114 pKa = 11.84VLNDD118 pKa = 2.91IADD121 pKa = 4.02EE122 pKa = 4.17QLVKK126 pKa = 11.36ANMTSAEE133 pKa = 4.34LEE135 pKa = 3.9LSEE138 pKa = 4.92TKK140 pKa = 10.41RR141 pKa = 11.84QEE143 pKa = 3.71AQRR146 pKa = 11.84ARR148 pKa = 11.84KK149 pKa = 9.86DD150 pKa = 3.3GTVRR154 pKa = 11.84PLVDD158 pKa = 4.22FDD160 pKa = 4.68HH161 pKa = 6.98GAIDD165 pKa = 5.05DD166 pKa = 4.05ADD168 pKa = 3.74EE169 pKa = 4.07

Molecular weight: 18.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q94ML4|Q94ML4_9VIRU Membrane protein P10 OS=Pseudomonas phage phi12 OX=161736 GN=10 PE=4 SV=1
MM1 pKa = 7.58GFFKK5 pKa = 10.64SIKK8 pKa = 9.53KK9 pKa = 9.98AIKK12 pKa = 9.58SVVKK16 pKa = 10.58AVVKK20 pKa = 9.79VVKK23 pKa = 10.3AVVKK27 pKa = 10.71AIVSVIKK34 pKa = 10.67AVVKK38 pKa = 10.12LIKK41 pKa = 10.38KK42 pKa = 8.58IVSVILDD49 pKa = 4.37FISKK53 pKa = 9.53IFSNIGVILLLILLVIFAWFLFPAFAAFLQSAWAWIVNIFSTGWAWVVKK102 pKa = 8.92TAGAAWQAITSFASTAWGYY121 pKa = 7.85ITAAWEE127 pKa = 4.3WIAGAVGFIWKK138 pKa = 9.3GLSGAADD145 pKa = 4.03AVWEE149 pKa = 4.04AAKK152 pKa = 10.49EE153 pKa = 3.98LGSAAGGVLSGVWDD167 pKa = 5.19FIKK170 pKa = 10.61EE171 pKa = 4.1NPEE174 pKa = 4.38LIAGGAALAWLGKK187 pKa = 10.28DD188 pKa = 3.32GLILVGLGVAAYY200 pKa = 9.41FLLKK204 pKa = 10.73SSGSKK209 pKa = 10.34GGGRR213 pKa = 11.84GEE215 pKa = 3.93QKK217 pKa = 10.7VVINTTGEE225 pKa = 3.94AYY227 pKa = 10.25APSPYY232 pKa = 8.97RR233 pKa = 11.84TGSGAPRR240 pKa = 11.84IAA242 pKa = 4.32

Molecular weight: 25.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

3788

43

723

252.5

27.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.032 ± 1.0

0.634 ± 0.105

5.913 ± 0.434

5.306 ± 0.536

4.039 ± 0.469

7.92 ± 0.55

1.716 ± 0.233

5.438 ± 0.405

5.121 ± 0.431

8.342 ± 0.456

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.194 ± 0.386

4.171 ± 0.407

3.907 ± 0.519

3.59 ± 0.423

5.253 ± 0.464

6.864 ± 0.463

5.966 ± 0.608

8.448 ± 0.694

1.346 ± 0.314

2.798 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski