Firmicutes bacterium CAG:240

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1917 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6I370|R6I370_9FIRM CBS domain protein OS=Firmicutes bacterium CAG:240 OX=1263013 GN=BN556_00233 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.15NLKK5 pKa = 10.13KK6 pKa = 9.45ITALIMALIMVFALCACSDD25 pKa = 3.9DD26 pKa = 5.34DD27 pKa = 4.17DD28 pKa = 6.04AKK30 pKa = 11.46GKK32 pKa = 10.21VEE34 pKa = 4.5PGTVEE39 pKa = 3.75TGAPSAEE46 pKa = 4.01PEE48 pKa = 4.1KK49 pKa = 10.21TLEE52 pKa = 4.45LGTLSGGKK60 pKa = 9.17YY61 pKa = 10.08ANDD64 pKa = 3.37YY65 pKa = 11.16FGFGCEE71 pKa = 6.24LDD73 pKa = 4.64DD74 pKa = 3.28QWTYY78 pKa = 11.95ADD80 pKa = 4.33EE81 pKa = 4.69DD82 pKa = 3.81QLLSMIQATADD93 pKa = 4.41LIDD96 pKa = 4.86DD97 pKa = 4.55EE98 pKa = 4.81NFKK101 pKa = 11.38DD102 pKa = 5.25DD103 pKa = 5.28ILDD106 pKa = 3.61ADD108 pKa = 3.94MFYY111 pKa = 11.71DD112 pKa = 4.02MMAVYY117 pKa = 10.44YY118 pKa = 10.75DD119 pKa = 4.2GVTSMNVVVQNIGAAYY135 pKa = 9.35GALLDD140 pKa = 4.14EE141 pKa = 4.57EE142 pKa = 4.91TMVDD146 pKa = 3.43EE147 pKa = 4.97TIKK150 pKa = 10.84VLPDD154 pKa = 3.09QLAAASMDD162 pKa = 3.68VQSCEE167 pKa = 3.83HH168 pKa = 5.33VTVEE172 pKa = 5.13FAGADD177 pKa = 3.11HH178 pKa = 7.55DD179 pKa = 5.69GILTHH184 pKa = 5.75STVNGGDD191 pKa = 3.44VYY193 pKa = 11.22QLQTYY198 pKa = 8.55VKK200 pKa = 8.17VGKK203 pKa = 9.85YY204 pKa = 7.9VAVVTLSSPLEE215 pKa = 4.58DD216 pKa = 4.57NLDD219 pKa = 3.62PMLGFFYY226 pKa = 10.7AVV228 pKa = 3.09

Molecular weight: 24.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6IG54|R6IG54_9FIRM Guanylate kinase OS=Firmicutes bacterium CAG:240 OX=1263013 GN=gmk PE=3 SV=1
MM1 pKa = 7.68LAQPHH6 pKa = 6.75LSRR9 pKa = 11.84SRR11 pKa = 11.84FPSPGRR17 pKa = 11.84PQRR20 pKa = 11.84TRR22 pKa = 11.84QTRR25 pKa = 11.84RR26 pKa = 11.84STPAAKK32 pKa = 10.17RR33 pKa = 11.84PLRR36 pKa = 11.84WSKK39 pKa = 10.83LSSIPLTRR47 pKa = 11.84WTAILCPFPLSSITQTAPSSLALPLTKK74 pKa = 10.26SAAFPLSFPSGLQRR88 pKa = 11.84PVHH91 pKa = 6.11SATSVHH97 pKa = 6.49SYY99 pKa = 10.36VRR101 pKa = 11.84MLPSVPSLLTRR112 pKa = 11.84KK113 pKa = 9.71RR114 pKa = 11.84PRR116 pKa = 11.84QLPKK120 pKa = 9.47MQTSLTSAPARR131 pKa = 11.84AKK133 pKa = 10.73ACTSTPWPFLRR144 pKa = 11.84STAWAAVSAA153 pKa = 4.41

Molecular weight: 16.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1917

0

1917

554504

33

2347

289.3

31.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.442 ± 0.069

1.955 ± 0.025

5.897 ± 0.048

6.602 ± 0.058

4.017 ± 0.04

7.666 ± 0.053

1.678 ± 0.023

6.804 ± 0.05

5.829 ± 0.047

9.109 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.082 ± 0.031

3.672 ± 0.034

3.625 ± 0.032

2.564 ± 0.027

4.911 ± 0.052

6.171 ± 0.054

5.217 ± 0.044

7.21 ± 0.042

0.913 ± 0.022

3.629 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski