Budgerigar fledgling disease virus (BFPyV) (Aves polyomavirus 1)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Gammapolyomavirus

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P13892-2|VP2-2_BFPYV Isoform of P13892 Isoform VP3 of Minor capsid protein VP2 OS=Budgerigar fledgling disease virus OX=1891747 PE=3 SV=3
MM1 pKa = 7.66ASLRR5 pKa = 11.84RR6 pKa = 11.84LTEE9 pKa = 4.0LLCLPVTATAADD21 pKa = 3.13IKK23 pKa = 9.38TAYY26 pKa = 9.93RR27 pKa = 11.84RR28 pKa = 11.84TALKK32 pKa = 9.55YY33 pKa = 10.52HH34 pKa = 7.07PDD36 pKa = 3.36KK37 pKa = 11.66GGDD40 pKa = 3.57EE41 pKa = 4.33EE42 pKa = 5.96KK43 pKa = 10.44MKK45 pKa = 10.57EE46 pKa = 4.23LNTLMEE52 pKa = 4.54EE53 pKa = 4.12FRR55 pKa = 11.84EE56 pKa = 4.48TEE58 pKa = 3.99GLRR61 pKa = 11.84ADD63 pKa = 3.92EE64 pKa = 4.41TLEE67 pKa = 4.74DD68 pKa = 4.05SDD70 pKa = 5.13PEE72 pKa = 4.28PEE74 pKa = 3.94EE75 pKa = 3.91SGYY78 pKa = 9.63ATFEE82 pKa = 4.22NVSVPDD88 pKa = 3.18IDD90 pKa = 4.05GAFFKK95 pKa = 10.88LMKK98 pKa = 10.11LKK100 pKa = 10.67KK101 pKa = 10.3CMQTYY106 pKa = 9.47FSVNEE111 pKa = 3.93RR112 pKa = 11.84RR113 pKa = 11.84KK114 pKa = 9.48QDD116 pKa = 2.93IRR118 pKa = 11.84PEE120 pKa = 3.91YY121 pKa = 10.69FEE123 pKa = 5.08LFKK126 pKa = 11.11AFQDD130 pKa = 4.26VPWKK134 pKa = 10.28VLEE137 pKa = 4.55DD138 pKa = 4.24FFTSEE143 pKa = 4.18MFF145 pKa = 3.93

Molecular weight: 16.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P13894|LT_BFPYV Large T antigen OS=Budgerigar fledgling disease virus OX=1891747 PE=3 SV=2
MM1 pKa = 7.76PNGTPTLNRR10 pKa = 11.84PLARR14 pKa = 11.84LALRR18 pKa = 11.84ITSCAHH24 pKa = 5.48QGLEE28 pKa = 3.75RR29 pKa = 11.84LIRR32 pKa = 11.84LLHH35 pKa = 6.34LLLHH39 pKa = 6.85LGRR42 pKa = 11.84SLLDD46 pKa = 3.37LFQTLRR52 pKa = 11.84TALTQPSTSIFSLTRR67 pKa = 11.84LPLLLPPLRR76 pKa = 11.84LLLLLLLPSPPRR88 pKa = 11.84QPALASRR95 pKa = 11.84LTQPNRR101 pKa = 11.84PNRR104 pKa = 11.84GRR106 pKa = 11.84LAKK109 pKa = 10.46

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

2

7

1839

79

587

262.7

29.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.722 ± 0.932

1.631 ± 0.547

4.948 ± 0.698

6.525 ± 0.894

3.48 ± 0.542

6.417 ± 1.075

2.066 ± 0.441

5.22 ± 0.567

4.894 ± 0.952

11.474 ± 1.983

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.393 ± 0.262

3.698 ± 0.315

5.982 ± 0.868

4.785 ± 0.645

7.123 ± 0.842

4.84 ± 0.43

7.015 ± 0.39

5.655 ± 0.745

1.033 ± 0.402

3.1 ± 0.476
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski