Lake Sarah-associated circular virus-34

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQP1|A0A140AQP1_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-34 OX=1685762 PE=3 SV=1
MM1 pKa = 7.61ARR3 pKa = 11.84KK4 pKa = 9.14CWCFTLNNYY13 pKa = 8.76TDD15 pKa = 4.38DD16 pKa = 4.29EE17 pKa = 4.65YY18 pKa = 11.79SAIIGRR24 pKa = 11.84LPDD27 pKa = 3.61LSRR30 pKa = 11.84YY31 pKa = 8.53YY32 pKa = 10.83VVGVEE37 pKa = 4.31VGDD40 pKa = 3.89SGTKK44 pKa = 9.91HH45 pKa = 5.28LQGYY49 pKa = 10.3ASFRR53 pKa = 11.84VAKK56 pKa = 10.09RR57 pKa = 11.84LNAVKK62 pKa = 10.54DD63 pKa = 3.44ILSNRR68 pKa = 11.84AHH70 pKa = 6.77CEE72 pKa = 3.44AARR75 pKa = 11.84GSAGQNRR82 pKa = 11.84KK83 pKa = 9.51YY84 pKa = 9.32CTKK87 pKa = 10.89DD88 pKa = 2.77GDD90 pKa = 4.03FVEE93 pKa = 5.33GGEE96 pKa = 4.63VPKK99 pKa = 8.88TTDD102 pKa = 2.62KK103 pKa = 10.46VTRR106 pKa = 11.84NDD108 pKa = 3.06LAKK111 pKa = 10.3QFRR114 pKa = 11.84EE115 pKa = 4.07AFAGGMSGVASFADD129 pKa = 4.1EE130 pKa = 4.86QPGTWFFSGHH140 pKa = 5.31NLLRR144 pKa = 11.84NTLTLMAPEE153 pKa = 4.63PRR155 pKa = 11.84PDD157 pKa = 3.09ISVRR161 pKa = 11.84WIYY164 pKa = 10.57GPPGVGKK171 pKa = 9.98SRR173 pKa = 11.84KK174 pKa = 8.77AHH176 pKa = 6.31EE177 pKa = 4.8EE178 pKa = 3.92LPDD181 pKa = 3.89AYY183 pKa = 9.78IKK185 pKa = 10.43EE186 pKa = 4.41PRR188 pKa = 11.84TKK190 pKa = 8.63WWNGYY195 pKa = 9.02YY196 pKa = 10.55GEE198 pKa = 4.61KK199 pKa = 9.61TCILDD204 pKa = 3.55DD205 pKa = 4.72FGPGGIDD212 pKa = 3.15INHH215 pKa = 7.45LLRR218 pKa = 11.84WFDD221 pKa = 3.45RR222 pKa = 11.84YY223 pKa = 10.77KK224 pKa = 11.0CLVEE228 pKa = 4.33NKK230 pKa = 10.39GGMVPLLVSTFIVTSNFHH248 pKa = 6.75PNQIFKK254 pKa = 10.61FGEE257 pKa = 4.09EE258 pKa = 3.97THH260 pKa = 6.51PQLPALEE267 pKa = 3.97RR268 pKa = 11.84RR269 pKa = 11.84ITLEE273 pKa = 3.58EE274 pKa = 4.04MEE276 pKa = 4.38

Molecular weight: 31.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQP1|A0A140AQP1_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-34 OX=1685762 PE=3 SV=1
MM1 pKa = 7.52ARR3 pKa = 11.84THH5 pKa = 4.59QTRR8 pKa = 11.84KK9 pKa = 9.25RR10 pKa = 11.84SRR12 pKa = 11.84PFVKK16 pKa = 9.32SRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84VRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84TFKK27 pKa = 10.24RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84NAIVTTSRR39 pKa = 11.84LLSASNPFGYY49 pKa = 9.82RR50 pKa = 11.84GRR52 pKa = 11.84RR53 pKa = 11.84LSKK56 pKa = 9.97KK57 pKa = 9.73RR58 pKa = 11.84YY59 pKa = 9.31RR60 pKa = 11.84SALWRR65 pKa = 11.84DD66 pKa = 3.65TLFKK70 pKa = 9.52THH72 pKa = 5.77YY73 pKa = 9.87RR74 pKa = 11.84SIRR77 pKa = 11.84SQSIALPIPNSNSGEE92 pKa = 4.25TVTFLQCFDD101 pKa = 3.73TTDD104 pKa = 2.9VTTFWKK110 pKa = 9.35TLGGLEE116 pKa = 3.95QANFSTGVPWDD127 pKa = 3.73AGTTNPEE134 pKa = 4.36SITIRR139 pKa = 11.84GGRR142 pKa = 11.84LWISANNRR150 pKa = 11.84SGQSEE155 pKa = 4.32SPRR158 pKa = 11.84IRR160 pKa = 11.84FQLVFLKK167 pKa = 10.89GALRR171 pKa = 11.84NSPDD175 pKa = 3.29TGQSNSAWDD184 pKa = 3.71YY185 pKa = 8.29LTSVVAGIKK194 pKa = 9.95PIGWDD199 pKa = 3.2VTNAPDD205 pKa = 3.38YY206 pKa = 11.7SMYY209 pKa = 10.45CHH211 pKa = 6.82PPVLSKK217 pKa = 11.22SFDD220 pKa = 3.17FRR222 pKa = 11.84ADD224 pKa = 3.53DD225 pKa = 3.61TCDD228 pKa = 3.17IFWKK232 pKa = 10.11IKK234 pKa = 9.08PVKK237 pKa = 9.68IDD239 pKa = 3.05TAAFTRR245 pKa = 11.84GGGWFPVWFVTMSTHH260 pKa = 6.84FDD262 pKa = 3.23ASTGSDD268 pKa = 3.31DD269 pKa = 5.33FKK271 pKa = 11.17IQLGHH276 pKa = 6.3NLSFAASDD284 pKa = 3.68LSSS287 pKa = 3.01

Molecular weight: 32.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

563

276

287

281.5

31.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.394 ± 0.093

1.599 ± 0.421

5.861 ± 0.047

4.085 ± 2.046

5.861 ± 0.577

7.993 ± 1.043

2.131 ± 0.296

4.618 ± 0.198

5.506 ± 0.477

6.927 ± 0.498

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.421 ± 0.286

4.44 ± 0.197

5.151 ± 0.207

2.487 ± 0.229

8.881 ± 1.46

7.993 ± 2.4

7.638 ± 1.345

5.329 ± 0.342

2.664 ± 0.358

3.02 ± 0.706
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski