Pseudoalteromonas phage J2-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae; Pseudoalteromonas virus J2-1

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 180 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GN13|A0A2D1GN13_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage J2-1 OX=2041340 PE=4 SV=1
MM1 pKa = 7.53SLTKK5 pKa = 10.07TLEE8 pKa = 3.7VDD10 pKa = 2.77ILTNTTSYY18 pKa = 10.98TSLPEE23 pKa = 4.23ALILDD28 pKa = 4.43DD29 pKa = 4.51EE30 pKa = 4.73NVVTVQATIKK40 pKa = 10.31DD41 pKa = 3.46ASGNMDD47 pKa = 2.7ITGITPSFEE56 pKa = 4.6INGVTQALNVLDD68 pKa = 4.09PTNGLVSYY76 pKa = 10.62DD77 pKa = 3.53VGITKK82 pKa = 10.5DD83 pKa = 3.56NYY85 pKa = 8.95PTTDD89 pKa = 3.33LYY91 pKa = 11.32KK92 pKa = 10.59VNLVLVQGATIANYY106 pKa = 8.91TGYY109 pKa = 8.11TFQVITLDD117 pKa = 3.39

Molecular weight: 12.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GNA5|A0A2D1GNA5_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage J2-1 OX=2041340 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 9.48EE3 pKa = 3.38KK4 pKa = 10.55LYY6 pKa = 10.88AVEE9 pKa = 4.14VTLDD13 pKa = 3.5DD14 pKa = 3.85GRR16 pKa = 11.84VYY18 pKa = 10.64YY19 pKa = 10.83ANPFRR24 pKa = 11.84GTISNRR30 pKa = 11.84NLVPFVVLSSAPRR43 pKa = 11.84VKK45 pKa = 10.61RR46 pKa = 11.84KK47 pKa = 9.88DD48 pKa = 3.34RR49 pKa = 11.84SEE51 pKa = 4.35EE52 pKa = 3.67EE53 pKa = 3.67WKK55 pKa = 10.92FLVKK59 pKa = 10.2RR60 pKa = 11.84RR61 pKa = 11.84NRR63 pKa = 11.84STSLRR68 pKa = 11.84NARR71 pKa = 11.84KK72 pKa = 9.3LRR74 pKa = 11.84NYY76 pKa = 10.87LSDD79 pKa = 3.66CKK81 pKa = 10.61KK82 pKa = 10.51ALEE85 pKa = 4.28KK86 pKa = 10.33RR87 pKa = 11.84HH88 pKa = 5.56PSIVEE93 pKa = 3.69FRR95 pKa = 11.84LVRR98 pKa = 11.84LGTKK102 pKa = 9.7ACPRR106 pKa = 11.84GNFLKK111 pKa = 10.58PNRR114 pKa = 11.84KK115 pKa = 9.46DD116 pKa = 3.13FRR118 pKa = 11.84YY119 pKa = 10.78YY120 pKa = 11.17LDD122 pKa = 3.39NCVNKK127 pKa = 10.39DD128 pKa = 2.78IYY130 pKa = 11.5LNGKK134 pKa = 9.73FSGCHH139 pKa = 6.14LISEE143 pKa = 4.87RR144 pKa = 11.84KK145 pKa = 8.8PLEE148 pKa = 3.98SFFKK152 pKa = 10.66EE153 pKa = 3.68RR154 pKa = 11.84AKK156 pKa = 11.06KK157 pKa = 10.64NIDD160 pKa = 3.29IKK162 pKa = 11.04DD163 pKa = 3.58SKK165 pKa = 11.13LL166 pKa = 3.38

Molecular weight: 19.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

180

0

180

41512

38

1563

230.6

26.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.678 ± 0.212

1.094 ± 0.071

6.656 ± 0.147

7.379 ± 0.239

4.367 ± 0.123

6.304 ± 0.193

1.812 ± 0.083

6.138 ± 0.121

7.092 ± 0.272

8.564 ± 0.172

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.45 ± 0.107

5.283 ± 0.13

3.334 ± 0.117

3.645 ± 0.093

4.107 ± 0.115

6.348 ± 0.151

6.504 ± 0.265

7.02 ± 0.154

1.255 ± 0.083

3.972 ± 0.1
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski