Odonata-associated circular virus-8

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UGS9|A0A0B4UGS9_9VIRU Replication-associated protein OS=Odonata-associated circular virus-8 OX=1592128 PE=3 SV=1
MM1 pKa = 8.06IEE3 pKa = 4.13DD4 pKa = 3.58VEE6 pKa = 4.85KK7 pKa = 11.06FFDD10 pKa = 3.69KK11 pKa = 10.52QDD13 pKa = 3.51KK14 pKa = 10.49KK15 pKa = 11.2KK16 pKa = 10.46EE17 pKa = 4.17GYY19 pKa = 9.61RR20 pKa = 11.84ATGKK24 pKa = 10.51AFFLTYY30 pKa = 9.05PKK32 pKa = 10.56CDD34 pKa = 3.25IKK36 pKa = 10.89RR37 pKa = 11.84DD38 pKa = 3.44EE39 pKa = 5.15AYY41 pKa = 10.07TMLAKK46 pKa = 9.96QWNPSNLIVAQEE58 pKa = 4.01DD59 pKa = 3.96HH60 pKa = 7.39KK61 pKa = 11.52DD62 pKa = 3.8GTPHH66 pKa = 6.29LHH68 pKa = 7.57VYY70 pKa = 8.13MQLPTKK76 pKa = 10.64KK77 pKa = 10.16NFKK80 pKa = 9.26NPRR83 pKa = 11.84WADD86 pKa = 3.12LGKK89 pKa = 10.22YY90 pKa = 9.21HH91 pKa = 7.49GFYY94 pKa = 8.15TTVRR98 pKa = 11.84DD99 pKa = 3.83RR100 pKa = 11.84QAVMAYY106 pKa = 9.71VMKK109 pKa = 10.81DD110 pKa = 3.08GNFKK114 pKa = 10.58MNEE117 pKa = 4.03NLNFTTWHH125 pKa = 6.25NFRR128 pKa = 11.84KK129 pKa = 10.11EE130 pKa = 3.96KK131 pKa = 10.87GDD133 pKa = 3.27FDD135 pKa = 3.3AWIIHH140 pKa = 5.77NEE142 pKa = 4.08SKK144 pKa = 10.58KK145 pKa = 10.85LKK147 pKa = 10.29SPYY150 pKa = 9.51PFKK153 pKa = 11.11LPDD156 pKa = 3.7GTDD159 pKa = 3.25VLEE162 pKa = 4.69PKK164 pKa = 10.56LGEE167 pKa = 4.17KK168 pKa = 9.81KK169 pKa = 9.83CNHH172 pKa = 6.61MIIGIPDD179 pKa = 3.39TGKK182 pKa = 10.46SFWYY186 pKa = 8.32NTTFEE191 pKa = 4.35GKK193 pKa = 9.77RR194 pKa = 11.84VFSRR198 pKa = 11.84VDD200 pKa = 2.96GKK202 pKa = 11.33YY203 pKa = 10.5PFDD206 pKa = 4.72DD207 pKa = 4.15YY208 pKa = 11.58EE209 pKa = 4.39GQEE212 pKa = 3.98VVIYY216 pKa = 10.41NDD218 pKa = 3.72PQTVTLMEE226 pKa = 5.61LINVSDD232 pKa = 4.65CYY234 pKa = 10.79LVKK237 pKa = 10.29TPVFGDD243 pKa = 3.06TRR245 pKa = 11.84YY246 pKa = 8.06TKK248 pKa = 10.26KK249 pKa = 9.6YY250 pKa = 8.63WKK252 pKa = 8.77MKK254 pKa = 7.8QRR256 pKa = 11.84RR257 pKa = 11.84TIIFICNHH265 pKa = 4.01GRR267 pKa = 11.84IPNFVEE273 pKa = 4.99DD274 pKa = 3.5PAFRR278 pKa = 11.84SRR280 pKa = 11.84FNIWDD285 pKa = 3.54MGDD288 pKa = 4.23DD289 pKa = 3.72EE290 pKa = 5.25PEE292 pKa = 4.41PEE294 pKa = 5.26EE295 pKa = 4.2MTDD298 pKa = 3.45DD299 pKa = 4.14PNRR302 pKa = 11.84TEE304 pKa = 4.08MDD306 pKa = 3.22TDD308 pKa = 4.59FDD310 pKa = 3.79ATDD313 pKa = 3.45YY314 pKa = 11.66ARR316 pKa = 4.91

Molecular weight: 37.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UGS9|A0A0B4UGS9_9VIRU Replication-associated protein OS=Odonata-associated circular virus-8 OX=1592128 PE=3 SV=1
MM1 pKa = 7.31SKK3 pKa = 10.62AAGHH7 pKa = 7.22LATTLAGLDD16 pKa = 3.36QIIRR20 pKa = 11.84SGKK23 pKa = 9.07SMYY26 pKa = 10.56NRR28 pKa = 11.84FSNWRR33 pKa = 11.84NPRR36 pKa = 11.84QVTPTRR42 pKa = 11.84SFPKK46 pKa = 9.41FSTRR50 pKa = 11.84MRR52 pKa = 11.84TISRR56 pKa = 11.84RR57 pKa = 11.84SFTKK61 pKa = 10.13SRR63 pKa = 11.84SSNGTRR69 pKa = 11.84KK70 pKa = 9.71RR71 pKa = 11.84SYY73 pKa = 9.16NGKK76 pKa = 9.66RR77 pKa = 11.84KK78 pKa = 9.88RR79 pKa = 11.84NSTKK83 pKa = 8.85RR84 pKa = 11.84TVWRR88 pKa = 11.84MKK90 pKa = 10.58KK91 pKa = 9.8KK92 pKa = 10.23LKK94 pKa = 10.45QIMVPAKK101 pKa = 10.41SLWLDD106 pKa = 3.47ALQGRR111 pKa = 11.84VTTGQGTSTWVSLGNLLTQNAVLTGFTGSVEE142 pKa = 4.12SGTYY146 pKa = 10.62LMSGLANNDD155 pKa = 3.23GTSGNILGRR164 pKa = 11.84AKK166 pKa = 10.61FIVHH170 pKa = 5.32SWEE173 pKa = 4.51QIFRR177 pKa = 11.84WTNQSNCPLTLEE189 pKa = 4.92CYY191 pKa = 7.95TLKK194 pKa = 10.56CVQNVPVDD202 pKa = 3.58GLINSGLEE210 pKa = 3.79VTNTIKK216 pKa = 11.09DD217 pKa = 3.69FMTACYY223 pKa = 9.9NKK225 pKa = 9.88QYY227 pKa = 10.97VGGSTIPSISVDD239 pKa = 3.15HH240 pKa = 6.87PAFKK244 pKa = 10.83LSDD247 pKa = 3.6LARR250 pKa = 11.84FGAYY254 pKa = 9.7FKK256 pKa = 10.13TIRR259 pKa = 11.84HH260 pKa = 5.15KK261 pKa = 9.79TVVVQPGEE269 pKa = 4.23SMSMRR274 pKa = 11.84KK275 pKa = 9.78YY276 pKa = 10.33KK277 pKa = 10.1IRR279 pKa = 11.84PRR281 pKa = 11.84TYY283 pKa = 10.95AEE285 pKa = 4.7AISYY289 pKa = 10.01DD290 pKa = 3.39AVTAVLKK297 pKa = 10.04VHH299 pKa = 5.75AQKK302 pKa = 9.85GTLHH306 pKa = 5.56YY307 pKa = 9.88LWRR310 pKa = 11.84ITGTPQSAAEE320 pKa = 4.41GKK322 pKa = 9.96DD323 pKa = 3.14ATLCDD328 pKa = 3.36VKK330 pKa = 10.53MAFVSSIHH338 pKa = 6.25ARR340 pKa = 11.84TSGYY344 pKa = 10.66SSNNYY349 pKa = 6.8QTFPSTTGLPVGLVIEE365 pKa = 4.75NIYY368 pKa = 10.5PGTSEE373 pKa = 4.75AKK375 pKa = 9.79VAAPVPP381 pKa = 3.93

Molecular weight: 42.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

697

316

381

348.5

39.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.452 ± 1.084

1.291 ± 0.017

5.882 ± 2.573

4.161 ± 1.421

4.878 ± 1.158

6.6 ± 0.799

2.152 ± 0.249

4.735 ± 0.008

8.608 ± 1.202

5.882 ± 0.951

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.156 ± 0.42

5.452 ± 0.16

4.878 ± 0.536

3.156 ± 0.409

6.026 ± 0.631

6.456 ± 2.778

8.895 ± 1.059

6.026 ± 0.631

2.009 ± 0.135

4.304 ± 0.497
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski