Desulfohalobium retbaense (strain ATCC 49708 / DSM 5692 / JCM 16813 / HR100)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfohalobiaceae; Desulfohalobium; Desulfohalobium retbaense

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2500 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C8X4E4|C8X4E4_DESRD Uncharacterized protein OS=Desulfohalobium retbaense (strain ATCC 49708 / DSM 5692 / JCM 16813 / HR100) OX=485915 GN=Dret_2134 PE=3 SV=1
MM1 pKa = 7.17QMSKK5 pKa = 10.52RR6 pKa = 11.84NEE8 pKa = 3.9RR9 pKa = 11.84GFTLIEE15 pKa = 3.96LLIVVAIIGILAAIAIPQFTKK36 pKa = 10.78YY37 pKa = 9.54KK38 pKa = 9.34ASAMEE43 pKa = 4.11SQLEE47 pKa = 4.37GNLSNCISILSAQFADD63 pKa = 4.25NGTASYY69 pKa = 10.86DD70 pKa = 3.59CSLGDD75 pKa = 3.37STVTLEE81 pKa = 4.06IGSDD85 pKa = 3.82GLVDD89 pKa = 3.67EE90 pKa = 5.36ATNSTDD96 pKa = 3.2SLSNIAVEE104 pKa = 4.87SYY106 pKa = 11.69DD107 pKa = 4.99DD108 pKa = 4.3ANCTLTTDD116 pKa = 4.26NDD118 pKa = 3.76VTDD121 pKa = 3.72IDD123 pKa = 4.4CTVEE127 pKa = 3.71

Molecular weight: 13.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C8X4G0|C8X4G0_DESRD Uncharacterized protein OS=Desulfohalobium retbaense (strain ATCC 49708 / DSM 5692 / JCM 16813 / HR100) OX=485915 GN=Dret_1899 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.34QPSTTKK11 pKa = 10.43RR12 pKa = 11.84KK13 pKa = 7.72RR14 pKa = 11.84THH16 pKa = 5.89GFLVRR21 pKa = 11.84SRR23 pKa = 11.84TKK25 pKa = 10.25NGRR28 pKa = 11.84AVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.44GRR39 pKa = 11.84KK40 pKa = 8.55RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2500

0

2500

812020

30

1706

324.8

35.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.526 ± 0.064

1.378 ± 0.024

5.228 ± 0.039

6.63 ± 0.046

3.906 ± 0.03

7.811 ± 0.041

2.39 ± 0.022

4.982 ± 0.046

3.968 ± 0.042

10.879 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.02

2.895 ± 0.031

4.962 ± 0.035

4.64 ± 0.035

6.524 ± 0.05

5.562 ± 0.034

5.2 ± 0.033

7.184 ± 0.042

1.429 ± 0.026

2.637 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski