Pseudoxanthomonas dokdonensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Pseudoxanthomonas

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3001 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0R0CQP3|A0A0R0CQP3_9GAMM Nucleotide-binding protein ABB29_14395 OS=Pseudoxanthomonas dokdonensis OX=344882 GN=ABB29_14395 PE=3 SV=1
MM1 pKa = 7.43KK2 pKa = 10.33SVNRR6 pKa = 11.84RR7 pKa = 11.84AALVASLLLVLPFATLAQDD26 pKa = 3.87AEE28 pKa = 4.44AEE30 pKa = 4.26AEE32 pKa = 4.29AEE34 pKa = 4.06ASSPISWSLGATTDD48 pKa = 3.49YY49 pKa = 11.21VFRR52 pKa = 11.84GLSQTDD58 pKa = 3.36EE59 pKa = 4.62GPAFQAGATYY69 pKa = 7.88TAPFGMYY76 pKa = 10.19AGVWGSNVDD85 pKa = 4.58FGPGDD90 pKa = 3.62PNYY93 pKa = 10.53EE94 pKa = 3.97LDD96 pKa = 3.56TFIGYY101 pKa = 7.68NTDD104 pKa = 3.82FGDD107 pKa = 3.73VVNFDD112 pKa = 3.39IMANRR117 pKa = 11.84YY118 pKa = 8.45NYY120 pKa = 9.88PDD122 pKa = 3.9AGKK125 pKa = 10.25SNYY128 pKa = 8.87WEE130 pKa = 5.42FIAKK134 pKa = 8.98TSFWEE139 pKa = 4.4NYY141 pKa = 8.89SVTVGYY147 pKa = 9.27TDD149 pKa = 5.42DD150 pKa = 4.27VFNSDD155 pKa = 2.88TDD157 pKa = 3.02GWYY160 pKa = 10.04YY161 pKa = 11.3AVGASFPLPADD172 pKa = 3.04ISLDD176 pKa = 3.48LSAGKK181 pKa = 10.14SEE183 pKa = 4.45FEE185 pKa = 4.47DD186 pKa = 4.74GEE188 pKa = 4.3VGRR191 pKa = 11.84DD192 pKa = 3.54YY193 pKa = 10.92MDD195 pKa = 2.95WSVALGKK202 pKa = 10.52SFGPLTVTAAYY213 pKa = 9.65IGTDD217 pKa = 2.99GDD219 pKa = 3.68GRR221 pKa = 11.84DD222 pKa = 3.79AYY224 pKa = 10.9GDD226 pKa = 3.46IADD229 pKa = 4.36DD230 pKa = 4.04RR231 pKa = 11.84FVLSASVGMM240 pKa = 4.72

Molecular weight:
25.9 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0R0CW75|A0A0R0CW75_9GAMM Endoribonuclease OS=Pseudoxanthomonas dokdonensis OX=344882 GN=ABB29_07275 PE=3 SV=1
MM1 pKa = 7.5AMKK4 pKa = 10.25KK5 pKa = 9.72AAKK8 pKa = 9.66KK9 pKa = 10.38KK10 pKa = 8.49PAKK13 pKa = 10.09KK14 pKa = 8.96ATKK17 pKa = 9.71KK18 pKa = 9.79AAKK21 pKa = 9.69KK22 pKa = 10.2AVAKK26 pKa = 9.01KK27 pKa = 7.44TAKK30 pKa = 10.18KK31 pKa = 9.81AAKK34 pKa = 9.54KK35 pKa = 9.06VAKK38 pKa = 10.15KK39 pKa = 10.22KK40 pKa = 8.81VAKK43 pKa = 10.08KK44 pKa = 9.05VAKK47 pKa = 9.98KK48 pKa = 9.19ATRR51 pKa = 11.84KK52 pKa = 8.54VAKK55 pKa = 10.1KK56 pKa = 8.95ATKK59 pKa = 9.74KK60 pKa = 9.18VAKK63 pKa = 10.14KK64 pKa = 9.97RR65 pKa = 11.84VAKK68 pKa = 10.06AAKK71 pKa = 9.54KK72 pKa = 9.99KK73 pKa = 9.06VAKK76 pKa = 10.09KK77 pKa = 9.17VAKK80 pKa = 10.2KK81 pKa = 9.04ATKK84 pKa = 9.74KK85 pKa = 9.18VAKK88 pKa = 10.14KK89 pKa = 9.97RR90 pKa = 11.84VAKK93 pKa = 10.06AAKK96 pKa = 9.54KK97 pKa = 9.99KK98 pKa = 9.06VAKK101 pKa = 10.15KK102 pKa = 9.14VAKK105 pKa = 10.09KK106 pKa = 9.88AAKK109 pKa = 9.54KK110 pKa = 8.91VAKK113 pKa = 10.21KK114 pKa = 10.07RR115 pKa = 11.84VAKK118 pKa = 9.81VAKK121 pKa = 9.91KK122 pKa = 10.11KK123 pKa = 9.19VAKK126 pKa = 10.02KK127 pKa = 9.05VAKK130 pKa = 10.23KK131 pKa = 10.16AVKK134 pKa = 9.85KK135 pKa = 9.88AAKK138 pKa = 9.78KK139 pKa = 10.26AVAKK143 pKa = 10.52KK144 pKa = 10.21AGKK147 pKa = 9.52KK148 pKa = 8.91AAKK151 pKa = 9.53KK152 pKa = 10.03AVRR155 pKa = 11.84KK156 pKa = 8.08VARR159 pKa = 11.84RR160 pKa = 11.84KK161 pKa = 9.42KK162 pKa = 9.26AAPVALPATPAPLII176 pKa = 4.24

Molecular weight:
18.65 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3001

0

3001

1002130

34

3821

333.9

36.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.479 ± 0.063

0.802 ± 0.016

6.051 ± 0.04

5.059 ± 0.042

3.335 ± 0.026

8.401 ± 0.047

2.255 ± 0.023

4.349 ± 0.033

2.779 ± 0.037

10.837 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.264 ± 0.02

2.777 ± 0.037

5.206 ± 0.038

4.58 ± 0.033

7.13 ± 0.053

5.746 ± 0.038

4.732 ± 0.053

7.209 ± 0.036

1.559 ± 0.02

2.451 ± 0.027

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski