Sporomusa acidovorans DSM 3132

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Sporomusaceae; Sporomusa; Sporomusa acidovorans

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5767 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A259UWA1|A0A259UWA1_9FIRM Uncharacterized protein OS=Sporomusa acidovorans DSM 3132 OX=1123286 GN=SPACI_14860 PE=4 SV=1
MM1 pKa = 7.74ASTFAGLSIANRR13 pKa = 11.84GLTAAQVGLTVTTNNMSNIDD33 pKa = 3.34TDD35 pKa = 4.26GYY37 pKa = 10.12SRR39 pKa = 11.84QVVSQTSIGPAAVYY53 pKa = 10.45SSSLVGNGVEE63 pKa = 4.26VTSVDD68 pKa = 3.23RR69 pKa = 11.84VNSFRR74 pKa = 11.84LNQKK78 pKa = 8.46YY79 pKa = 7.33WQEE82 pKa = 3.82NSSASEE88 pKa = 4.24LEE90 pKa = 4.14VTSNYY95 pKa = 8.47MEE97 pKa = 5.26KK98 pKa = 9.87IEE100 pKa = 5.1DD101 pKa = 3.66VFGTTDD107 pKa = 2.89SSDD110 pKa = 2.92ISEE113 pKa = 4.56ALDD116 pKa = 3.34TFEE119 pKa = 6.81AEE121 pKa = 4.99LEE123 pKa = 4.27TLADD127 pKa = 4.08DD128 pKa = 4.24PTNEE132 pKa = 4.42SIRR135 pKa = 11.84KK136 pKa = 8.83IVLSYY141 pKa = 11.2AKK143 pKa = 10.18SFCDD147 pKa = 3.6TLNTAAEE154 pKa = 4.29DD155 pKa = 3.85LEE157 pKa = 4.82SIRR160 pKa = 11.84DD161 pKa = 3.71QLNTEE166 pKa = 3.92VNTAVEE172 pKa = 4.88EE173 pKa = 4.31INSYY177 pKa = 10.87ASQIAEE183 pKa = 4.49LNRR186 pKa = 11.84QISTASASGASTNEE200 pKa = 4.04LEE202 pKa = 4.45DD203 pKa = 3.51QRR205 pKa = 11.84DD206 pKa = 3.92VIVDD210 pKa = 3.65KK211 pKa = 11.37LSGLVGISVTEE222 pKa = 4.4ADD224 pKa = 4.09DD225 pKa = 3.47STYY228 pKa = 10.57TITVDD233 pKa = 3.73GVTLVKK239 pKa = 10.57GDD241 pKa = 3.73EE242 pKa = 4.17ANEE245 pKa = 4.11LEE247 pKa = 5.3CYY249 pKa = 9.12TVTDD253 pKa = 4.24SDD255 pKa = 3.87SDD257 pKa = 4.01SYY259 pKa = 11.94GMYY262 pKa = 10.22GIRR265 pKa = 11.84WAEE268 pKa = 3.87SGKK271 pKa = 10.48DD272 pKa = 3.59FDD274 pKa = 5.14SGDD277 pKa = 3.75SGSLTGYY284 pKa = 10.23LQMRR288 pKa = 11.84DD289 pKa = 3.22GSTADD294 pKa = 3.25SKK296 pKa = 11.65GVVYY300 pKa = 10.13YY301 pKa = 9.66MNQLDD306 pKa = 4.5KK307 pKa = 10.73FAQTFAQAFNEE318 pKa = 4.54GVTVTSDD325 pKa = 2.66SGTEE329 pKa = 3.9TTYY332 pKa = 10.85SGHH335 pKa = 5.85VDD337 pKa = 3.35GVGIDD342 pKa = 3.95DD343 pKa = 5.96DD344 pKa = 4.23EE345 pKa = 4.63TTGIRR350 pKa = 11.84FFTYY354 pKa = 10.77DD355 pKa = 4.0DD356 pKa = 3.6VSSADD361 pKa = 3.83FVDD364 pKa = 3.8SGSDD368 pKa = 3.57LEE370 pKa = 4.25EE371 pKa = 4.77AYY373 pKa = 11.41SNITAANISVSKK385 pKa = 10.42DD386 pKa = 3.14IQDD389 pKa = 3.84DD390 pKa = 3.96TTKK393 pKa = 10.42IAASSTAGEE402 pKa = 4.22EE403 pKa = 4.57SNTDD407 pKa = 3.48VLDD410 pKa = 4.31DD411 pKa = 5.74LIDD414 pKa = 3.58ICSDD418 pKa = 3.44VNISGNSTATEE429 pKa = 4.17MYY431 pKa = 9.55NLIVATVAGDD441 pKa = 3.6SASAEE446 pKa = 4.06TAYY449 pKa = 10.74SSKK452 pKa = 10.83SATATYY458 pKa = 10.47INTSRR463 pKa = 11.84TSVSGVSSDD472 pKa = 3.68EE473 pKa = 3.94EE474 pKa = 4.74TVNLTTYY481 pKa = 10.05QSAYY485 pKa = 9.41AASASMVSAWSEE497 pKa = 3.97IYY499 pKa = 8.98DD500 pKa = 3.8TTISMVDD507 pKa = 3.41DD508 pKa = 3.98

Molecular weight: 54.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A259V1T0|A0A259V1T0_9FIRM Amino-acid permease RocC OS=Sporomusa acidovorans DSM 3132 OX=1123286 GN=rocC_1 PE=4 SV=1
MM1 pKa = 7.8AFTRR5 pKa = 11.84SLSLSLVKK13 pKa = 10.58QGIRR17 pKa = 11.84VNGVAPGPIWTPLIPASFSDD37 pKa = 3.23RR38 pKa = 11.84HH39 pKa = 4.42VARR42 pKa = 11.84FGRR45 pKa = 11.84NTPMKK50 pKa = 10.33RR51 pKa = 11.84AGQPAEE57 pKa = 3.92VAPCYY62 pKa = 10.78VFLASQDD69 pKa = 3.57SVYY72 pKa = 10.51MSGQILHH79 pKa = 6.5VNGGTIVNGG88 pKa = 3.67

Molecular weight: 9.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5767

0

5767

1704535

29

4642

295.6

32.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.217 ± 0.04

1.33 ± 0.018

4.854 ± 0.025

6.104 ± 0.035

3.887 ± 0.022

7.436 ± 0.035

1.857 ± 0.014

7.433 ± 0.03

6.012 ± 0.029

9.756 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.776 ± 0.016

4.204 ± 0.029

3.961 ± 0.022

3.878 ± 0.023

4.565 ± 0.028

5.507 ± 0.027

5.54 ± 0.028

7.328 ± 0.029

1.017 ± 0.014

3.338 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski