Bifidobacterium castoris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2051 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A430F658|A0A430F658_9BIFI Signal peptide protein OS=Bifidobacterium castoris OX=2306972 GN=D2E22_1350 PE=4 SV=1
MM1 pKa = 7.33VSYY4 pKa = 10.82DD5 pKa = 3.58DD6 pKa = 4.52AVAIIQDD13 pKa = 3.81PQADD17 pKa = 4.1PVTLAKK23 pKa = 10.04VAYY26 pKa = 7.25EE27 pKa = 3.96NPEE30 pKa = 3.71FGANVAAHH38 pKa = 5.94PRR40 pKa = 11.84AYY42 pKa = 9.64PGLLRR47 pKa = 11.84WIAQFGDD54 pKa = 3.06EE55 pKa = 4.32RR56 pKa = 11.84ARR58 pKa = 11.84TTVAQLGYY66 pKa = 9.58QAQLGAVEE74 pKa = 4.93DD75 pKa = 4.2RR76 pKa = 11.84QVDD79 pKa = 3.91DD80 pKa = 4.09AAMDD84 pKa = 3.61EE85 pKa = 4.3AAAIATAIDD94 pKa = 3.52AAQANAAARR103 pKa = 11.84PAANNAAAGLVMDD116 pKa = 5.53AASAGANSHH125 pKa = 7.22PIASQTGTVEE135 pKa = 4.1YY136 pKa = 10.43ASAEE140 pKa = 3.98AQPVSFEE147 pKa = 4.21PAQQTGVDD155 pKa = 3.65MLSAHH160 pKa = 6.75FDD162 pKa = 4.47DD163 pKa = 5.22IGMDD167 pKa = 3.8ASQTNMDD174 pKa = 3.97ASQPALSPAVAAPDD188 pKa = 3.57MAQAEE193 pKa = 4.41QPAVDD198 pKa = 3.71QAQPAQAYY206 pKa = 9.57AQIQATNPYY215 pKa = 10.02GFTAEE220 pKa = 4.18IAMTTPDD227 pKa = 3.9TTVMQQIAQYY237 pKa = 10.91APEE240 pKa = 4.46LHH242 pKa = 6.85PALAQNPYY250 pKa = 10.19IYY252 pKa = 10.11PEE254 pKa = 4.11LLNWLGMLGEE264 pKa = 4.45PATNASIAQRR274 pKa = 11.84QQHH277 pKa = 5.17

Molecular weight: 28.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A430F8D3|A0A430F8D3_9BIFI Diacetyl reductase [(S)-acetoin forming] OS=Bifidobacterium castoris OX=2306972 GN=D2E22_1016 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.79MKK15 pKa = 9.36HH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84ALINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.02GRR39 pKa = 11.84KK40 pKa = 8.83ALSAA44 pKa = 3.99

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2051

0

2051

719393

29

3117

350.8

38.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.805 ± 0.077

1.053 ± 0.02

7.226 ± 0.048

5.598 ± 0.047

3.206 ± 0.037

8.103 ± 0.048

2.225 ± 0.023

4.97 ± 0.041

3.281 ± 0.042

8.592 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.736 ± 0.026

2.938 ± 0.033

4.811 ± 0.038

3.309 ± 0.037

6.8 ± 0.069

5.363 ± 0.042

6.089 ± 0.047

7.758 ± 0.051

1.444 ± 0.021

2.693 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski