Moorella thermoacetica (strain ATCC 39073 / JCM 9320)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacteraceae; Moorella group; Moorella; Moorella thermoacetica

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2451 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2RHJ5|Q2RHJ5_MOOTA Uncharacterized protein OS=Moorella thermoacetica (strain ATCC 39073 / JCM 9320) OX=264732 GN=Moth_1793 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 9.98VTVDD6 pKa = 3.21QDD8 pKa = 3.57LCIACGTCIDD18 pKa = 4.66LCPSVFDD25 pKa = 4.33WDD27 pKa = 4.6DD28 pKa = 3.34EE29 pKa = 4.55GLSHH33 pKa = 7.26VIVDD37 pKa = 4.36EE38 pKa = 4.38VPEE41 pKa = 4.31GAEE44 pKa = 3.77DD45 pKa = 3.82CARR48 pKa = 11.84EE49 pKa = 4.3SVNSCPTEE57 pKa = 4.27AIKK60 pKa = 10.47EE61 pKa = 4.26VV62 pKa = 3.36

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2RK37|Q2RK37_MOOTA Uncharacterized protein OS=Moorella thermoacetica (strain ATCC 39073 / JCM 9320) OX=264732 GN=Moth_0885 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84IKK4 pKa = 10.49KK5 pKa = 10.02RR6 pKa = 11.84LFIGLLALSLMFITAVLAGSWYY28 pKa = 10.87LLINHH33 pKa = 6.49SSLFNRR39 pKa = 11.84VLLALGFFTLAVLFLLIALGIISLVLMLWQGRR71 pKa = 11.84SRR73 pKa = 11.84PLFQHH78 pKa = 6.73LGLMAVNILFPVALALGKK96 pKa = 10.48RR97 pKa = 11.84LGVEE101 pKa = 3.49AATIKK106 pKa = 10.91ASFIEE111 pKa = 4.33MNNQLVRR118 pKa = 11.84LQRR121 pKa = 11.84LQVAPRR127 pKa = 11.84EE128 pKa = 4.1ILILAPHH135 pKa = 6.89CLQWSGCPHH144 pKa = 7.73KK145 pKa = 10.35ITIDD149 pKa = 3.4VNNCRR154 pKa = 11.84RR155 pKa = 11.84CGRR158 pKa = 11.84CPIDD162 pKa = 4.06ALHH165 pKa = 6.88ALAARR170 pKa = 11.84YY171 pKa = 8.36GVRR174 pKa = 11.84LAVATGGTLARR185 pKa = 11.84HH186 pKa = 5.71FVKK189 pKa = 10.35QYY191 pKa = 9.97RR192 pKa = 11.84PRR194 pKa = 11.84AVVAIACEE202 pKa = 3.97RR203 pKa = 11.84DD204 pKa = 3.59LTSGIQDD211 pKa = 3.71TQPLPVLGVLNLRR224 pKa = 11.84PHH226 pKa = 6.8GPCLNTQVNLNQVEE240 pKa = 4.16QAVQFFLTGRR250 pKa = 11.84TVPQVQACSEE260 pKa = 4.05GWVEE264 pKa = 4.23VTHH267 pKa = 7.13GSS269 pKa = 3.31

Molecular weight: 29.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2451

0

2451

751866

37

1487

306.8

33.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.415 ± 0.057

1.059 ± 0.024

4.532 ± 0.034

6.764 ± 0.053

3.427 ± 0.031

8.59 ± 0.046

1.739 ± 0.019

6.04 ± 0.043

4.285 ± 0.044

11.234 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.242 ± 0.023

3.04 ± 0.028

5.029 ± 0.027

3.548 ± 0.035

6.867 ± 0.055

4.36 ± 0.034

5.007 ± 0.03

7.666 ± 0.045

1.13 ± 0.021

3.027 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski