Herbinix luporum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Herbinix

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2352 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K8J4C3|A0A0K8J4C3_9FIRM Putative secreted protein OS=Herbinix luporum OX=1679721 GN=SD1D_0643 PE=4 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84KK3 pKa = 9.58FEE5 pKa = 4.39CTACGYY11 pKa = 10.27IYY13 pKa = 10.36DD14 pKa = 4.79EE15 pKa = 4.71EE16 pKa = 6.36LGDD19 pKa = 4.32PDD21 pKa = 4.68NGVAPGTKK29 pKa = 8.96WEE31 pKa = 4.78DD32 pKa = 3.53VPEE35 pKa = 4.38DD36 pKa = 4.09WVCPLCGVGKK46 pKa = 10.55EE47 pKa = 4.39DD48 pKa = 4.4FEE50 pKa = 4.67EE51 pKa = 4.64VNN53 pKa = 3.65

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K8J6E9|A0A0K8J6E9_9FIRM Putative secreted protein OS=Herbinix luporum OX=1679721 GN=SD1D_1423 PE=3 SV=1
MM1 pKa = 7.34SCSGNFGFRR10 pKa = 11.84HH11 pKa = 6.6DD12 pKa = 3.67SCQRR16 pKa = 11.84KK17 pKa = 9.43HH18 pKa = 6.23NDD20 pKa = 2.74RR21 pKa = 11.84SFNRR25 pKa = 11.84FDD27 pKa = 3.58RR28 pKa = 11.84FDD30 pKa = 3.63RR31 pKa = 11.84RR32 pKa = 11.84HH33 pKa = 4.6SQCRR37 pKa = 11.84YY38 pKa = 9.02FNCNNKK44 pKa = 9.54RR45 pKa = 11.84PRR47 pKa = 11.84YY48 pKa = 9.44DD49 pKa = 4.06KK50 pKa = 11.0NDD52 pKa = 3.5CQVCPRR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84SRR62 pKa = 11.84FPIFPNFFF70 pKa = 3.86

Molecular weight: 8.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2352

0

2352

754026

30

1996

320.6

36.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.329 ± 0.05

1.117 ± 0.019

5.851 ± 0.038

7.224 ± 0.053

4.128 ± 0.034

6.393 ± 0.046

1.576 ± 0.018

9.229 ± 0.053

8.014 ± 0.045

9.293 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.708 ± 0.024

5.425 ± 0.033

3.183 ± 0.027

2.764 ± 0.026

3.931 ± 0.036

6.15 ± 0.042

5.143 ± 0.042

6.204 ± 0.035

0.799 ± 0.017

4.538 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski